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[1]吕早生,关海燕,李凌凌,等.一株高浸磷嗜酸氧化硫硫杆菌的分离与鉴定[J].应用与环境生物学报,2011,17(03):326-329.[doi:10.3724/SP.J.1145.2011.00326]
　LÜ,Zaosheng,GUAN Haiyan,et al.Isolation and Identifaction of Acidithiobacillus thiooxidans with Strong Phosphorous Ore Bioleaching Ability[J].Chinese Journal of Applied & Environmental Biology,2011,17(03):326-329.[doi:10.3724/SP.J.1145.2011.00326]
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一株高浸磷嗜酸氧化硫硫杆菌的分离与鉴定()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
17卷
期数:
2011年03期
页码:
326-329
栏目:
研究论文
出版日期:
2011-06-24

文章信息/Info

Title:
Isolation and Identifaction of Acidithiobacillus thiooxidans with Strong Phosphorous Ore Bioleaching Ability
作者:
吕早生; 关海燕; 李凌凌; 贾伟伟
（武汉科技大学化学工程与技术学院 武汉 430081）
Author(s):
LÜ Zaosheng;  GUAN Haiyan;  LI Lingling;   JIA Weiwei
(College of Chemical Engineering and Technology, Wuhan University of Science and Technology, Wuhan 430081, China)
关键词:
嗜酸氧化硫硫杆菌; 菌种筛选; 16S rDNA; 生物浸磷; 黄铁矿
Keywords:
Acidithiobacillus thiooxidans;  strain screening;  16S rDNA;  bioleaching;  pyrite
分类号:
TD853.37
DOI:
10.3724/SP.J.1145.2011.00326
文献标志码:
A
摘要:
从矿山土样中分离到一株嗜酸的浸磷矿细菌HY-01，观察其形态并研究其培养特征. 结果显示，该菌株为革兰氏阴性、短杆状运动细菌，菌体长1~2 μm，宽0.5~0.7 μm，能在pH 1.5~8.0的范围内生长，最适生长温度为37 ℃，最适pH为1.5~3.0. 该菌株可以KNO3、NH4NO3、(NH4)2CO3、NH4H2PO4、(NH4)2SO4以及蛋白胨为氮源，以元素硫和黄铁矿中的硫为能源，不能利用有机物质以及硫代硫酸钠、硫酸亚铁等无机物为能源生长. 形态、生理生化特性研究以及16S rDNA序列分析表明，该菌株为嗜酸氧化硫硫杆菌. 浸矿实验结果表明其具有很高的磷矿浸出能力. 图6 表1 参18
Abstract:
Acidophilic sulfur-oxidizing bacterium HY-01 was isolated from mine soil. Strain HY-01 was motile, gram-negative, rod-shaped and 0.5 to 0.7 μm × 1 to 2 μm in size. The strain grew optimally with initial pH 2.0~2.5 and temperature 37 ℃. It could grow with potassium nitrate, ammonium nitrate, ammonirm carbonate, ammonium dihydrogen phosphate, ammonium sulfate and tryptone as nitrogen sources. It could use elemental sulfur and pyrite as energy sources, but not organic minerals and inorganic minerals, including sodium thiosulfate and ferrous sulfate. The morphological, biochemical and physiological characteristics of the strain and the analysis based on 16S rDNA gene sequence indicated that strain HY-01 was most closely related to Acidithiobacillus thiooxidans. The sulfur-oxidizing bacteria could positively increase the dissolution rate and percentage of phosphorus recovery from phosphate ore. Fig 6, Tab 1, Ref 18

参考文献/References:

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备注/Memo

备注/Memo:
国家“973”基础研究项目（No. 2008CB617611）和湖北省科技厅基金项目（No. 2009CAD006）资助
更新日期/Last Update: 2011-06-23