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 LUO Xiaoli,NIU Xiangli,HUANG Weizao,et al.Expression Characteristics and Functional Analysis of Rice DDB1 Gene[J].Chinese Journal of Applied & Environmental Biology,2011,17(01):1-6.[doi:10.3724/SP.J.1145.2011.00001]





Expression Characteristics and Functional Analysis of Rice DDB1 Gene
四川大学生命科学学院生物资源与生态环境教育部重点实验室 成都 610064
LUO XiaoliNIU XiangliHUANG WeizaoLIU Yongsheng
(Key Laboratory for Bio-resource and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China)
DDB1 RNA interference fruit-specific promoter Agrobacterium-mediated transformation male semi-sterile rice
S511.03 : Q943.2
利用RNA干涉技术构建水稻DDB1(Damaged DNA binding protein 1)基因不同启动子驱动植物表达载体,DDB1-RNAi和DDB1-glu-RNAi. 通过根癌农杆菌介导转入水稻愈伤,经组织培养成功获得转基因植株. 半定量RT-PCR分析显示,与野生型植株相比,两组转基因植株幼叶内DDB1的表达量均有不同程度的降低. 花粉染色结果显示,转基因植株花粉染色异常,败育率高;植株表现出明显的雄性半不育;秕谷率均有不同程度的提高;并且以上表型在DDB1-RNAi转基因植株中均比在DDB1-glu-RNAi转基因植株中明显. 因此,推断DDB1基因与水稻的生殖发育有着密切的关系. 图6 表1 参30
In this study, two RNA interference (RNAi) vectors of rice DDB1 gene, DDB1-RNAi and DDB1-glu-RNAi, droved by different promoters were constructed. They were introduced into rice cv. Nipponbare by Agrobacterium-mediated transformation. The analysis of semi-quantitative RT-PCR revealed the variation of suppression of DDB1 in both RNAi transgenic lines compared to that of wild type. I2-KI indicated abnormally stained pollens, high level male semi-sterile in two sets of transgenic plants, and DDB1-RNAi transformed lines showed more severe sterility compared with that of DDB1-glu-RNAi ones. Consequently, DDB1 may be involved in reproductive development in rice. Fig 6, Tab 1, Ref 30


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国家转基因生物新品种培育科技重大专项课题(Nos. 2009ZX08009-072B,2009ZX08001-011B)和国家杰出青年科学基金项目(No. 30825030)资助
更新日期/Last Update: 2011-02-28