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[1]李岩,王海燕,张义正.甘薯蔗糖转运蛋白IbSUT1x在酵母细胞中的定位[J].应用与环境生物学报,2010,16(06):798-802.[doi:10.3724/SP.J.1145.2010.00798]
 LI Yan,WANG Haiyan,ZHANG Yizheng.Localization of IbSUT1x Protein from Ipomoea batatas (L.) Lam in Yeast Cells[J].Chinese Journal of Applied & Environmental Biology,2010,16(06):798-802.[doi:10.3724/SP.J.1145.2010.00798]
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甘薯蔗糖转运蛋白IbSUT1x在酵母细胞中的定位()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
16卷
期数:
2010年06期
页码:
798-802
栏目:
研究论文
出版日期:
2010-12-25

文章信息/Info

Title:
Localization of IbSUT1x Protein from Ipomoea batatas (L.) Lam in Yeast Cells
作者:
李岩王海燕 张义正
(1四川大学生命科学学院,生物资源与生态环境教育部重点实验室,四川省分子生物学与生物技术重点实验室 成都 610064)
(2山东农业大学农学院,作物生物学国家重点实验室,山东省作物生物学重点实验室 泰安 271018)
Author(s):
LI Yan WANG Haiyan ZHANG Yizheng
(1Key Laboratory of Biology Resource & Eco-environment, Ministry of Education, Sichuan Key Laboratory of Molecular Biology & Biotechnology,
College of Life Sciences, Sichuan University, Chengdu 610064, China)
(2State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Taian 271018, China)
关键词:
甘薯蔗糖转运蛋白基因表达定位绿色荧光蛋白酿酒酵母
Keywords:
Ipomoea batatas sucrose transporter gene expression localization GFP Saccharomyces cerevisiae
分类号:
Q786
DOI:
10.3724/SP.J.1145.2010.00798
文献标志码:
A
摘要:
蔗糖转运蛋白是植物特有的一类载体蛋白,酿酒酵母突变株SUSY7/ura3常被利用来研究蔗糖转运蛋白的功能,但在研究中发现,有的蔗糖转运蛋白在酵母中未表现出蔗糖转运的功能. 但这或许并不是其实际功能的体现,而是由于这类蛋白无法准确定位到酵母的细胞膜上造成的. IbSUT1x是本实验室从甘薯中分离到的一个蔗糖转运蛋白基因,但是在SUSY7/ura3中未表现出明显的蔗糖转运功能. 本研究通过用激光共聚焦显微镜对IbSUT1x与绿色荧光蛋白(Green fluorescent protein,GFP)的融合蛋白的表达和定位的观察,发现IbSUT1x不仅在酵母细胞中有表达,而且表达的蛋白质定位在酵母的细胞膜上. 结果表明,IbSUT1x在酵母细胞中不能转运蔗糖,该蛋白很可能不具有蔗糖转运功能,或者其与蔗糖的亲和力很低. 图4 表1 参21
Abstract:
Sucrose transporters (SUTs), or termed as sucrose carriers (SUCs), only exist in plants and play important roles in long-distance transport of photosynthate through phloem from source organs to sink ones. Its function was always studied with yeast (Saccharomyces cerevisiae) strain SUSY7/ura3 which is usually used as a model for sucrose uptake activity research. Researchers found that some sucrose transporters could not transport sucrose, which might be on account of the failed localization (not target to plasma membrane) in yeast cells. IbSUT1x is a sucrose transporter gene isolated from sweet potato (Ipomoea batatas), and it does not display apparent sucrose uptake activity. In the present study, it was revealed that IbSUT1x could be successfully targeted to plasma membrane of yeast cells by the localization of IbSUT1x-green fluorescent protein (GFP) fusion protein using laser scanning confocal microscopy. The result suggestes that IbSUT1x can not load sucrose into yeast cells, or just has low sucrose affinity. Fig 4, Tab 1, Ref 21

参考文献/References:

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备注/Memo

备注/Memo:
国家科技支撑计划项目(No. 2007BAD78B03)和四川省“十一五”重点攻关项目(No. 07SG111-003-1)资助
更新日期/Last Update: 2010-12-29