|本期目录/Table of Contents|

 ZHANG Xiaoxia,QIU Fubin,SUN Lei,et al.Phylogenetic Analysis of Endophytic Ensifer adhaerens Isolated from Rice Roots[J].Chinese Journal of Applied & Environmental Biology,2010,16(06):779-783.[doi:10.3724/SP.J.1145.2010.00779]





Phylogenetic Analysis of Endophytic Ensifer adhaerens Isolated from Rice Roots
(1中国农业科学院资源与区划研究所农业部作物营养与施肥重点实验室 北京 100081)
(2首都师范大学生命科学学院 北京 100048)
(3山西医科大学公共卫生学院 太原 030001)
(4河北大学生命科学学院河北省微生物多样性研究与应用实验室 保定 071002)
ZHANG Xiaoxia QIU Fubin SUN Lei MA Xiaotong JIANG Ruibo SONG Wei
(1Key Laboratory of Crop Nutrition and Fertilization, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, China)
(2College of Life Sciences, Capital Normal University, Beijing 100048, China)
(3College of Public Health, Shanxi Medical University, Taiyuan 030001, China)
endophytic bacterium from rice Ensifer adhaerens phylogenetic analysis nodulation test
通过16S rRNA基因和看家基因recA、atpD、rpoB的序列测定与分析,及(G+C) mol%测定等技术,对分离自河北省滦南县水稻根内的两株细菌进行分子系统发育学研究. 根据系统进化树构建及其同源性分析,可确定所分离的两株水稻内生细菌属于剑菌属(Ensifer)系统发育分支;供试菌株的各保守序列与粘着剑菌(E. adhaerens)模式菌种同源性最高,除recA为97.2%外,其他均为99%~100%,可以确定供试菌属于E. adhaerens;对所分离的菌株与大豆、菜豆、三叶草、苜蓿等4种豆科植物进行结瘤试验,结果显示其中一株J3-N55可与苜蓿结瘤. 野生型的E. adhaerens菌株与豆科植物结瘤的报道尚为鲜见;本文首次报道从水稻植株内分离到E. adhaerens菌株. 图4 表1 参22
Two Ensifer (Sinorhizobium) -like strains isolated from rice (Oryza sativa L.) roots in Luannan County, Hebei, China were investigated with a polyphasic strategy. The 16S rRNA, recA, atpD and rpoB genes were PCR amplified and sequenced, and followed by Neighbour-Joining phylogenetic analysis. The G+C content was determined by thermal denaturation method. According to the study of phylogenetics, the two strains were clustered in the genus Ensifer (Sinorhizobium), with the highest similarity to E. adhaerens ranging from 99% to 100%, except for recA gene (97.2%). To determine the nodulation ability, the nodulation tests were carried out using four different legumes: soybean (Glycine max), kidney bean (Phaseolus vulgaris), clover (Trifolium repens), and lucern (Medicago sativa). It was found that strain J3-N55 could form nodules successfully on M. sativa. To our knowledge, this is the first report about the endophytic E. adhaerens isolated from rice plant. Fig 4, Tab 1, Ref 22


1 Jr Casida LE. Ensifer adhaerens gen. nov., sp. nov.: A bacterial predator of bacteria in soil. Int J Syst Bacteriol, 1982, 32(3): 339~345
2 Willems A, Fernandez-Lopez M, Munoz-Adelantado E, Goris J, De Vos P, Martinez-Romero E, Toro N and Gillis M. Description of new Ensifer strains from nodules and proposal to transfer Ensifer adhaerens Casida 1982 to Sinorhizobium as Sinorhizobium adhaerens comb. nov. Request for an Opinion. Int J Syst Evol Microbiol, 2003, 53:1207~1217
3 Martens M, Dawyndt P, Coopman R, Gillis M, De Vos P, Willems A. Advantages of multilocus sequence analysis for taxonomic studies: A case study using 10 housekeeping genes in the genus Ensifer (including former Sinorhizobium). Int J Syst Evol Microbiol, 2008, 58: 200~214
4 Lapage SP, Sneath PHA, Lessel EF, Skerman VBD, Seeliger HPR, Clark WA. International Code of Nomenclature of Bacteria. American Society of Microbiology, Washington, USA, 1990
5 Young JM, Kuykendall LD, Martinez-Romero E, Kerr A, Sawada H. Classification and nomenclature of Agrobacterium and Rhizobium - A reply to Farrand et al. Int J Syst Evol Microbiol, 2003, 53: 1689~1695
6 Judicial Commission of the International Committee on Systematics of Prokaryotes. The genus name Sinorhizobium Chen et al. 1988 is a later synonym of Ensifer Casida 1982 and is not conserved over the latter genus name, and the species name ‘Sinorhizobium adhaerens’ is not validly published. Opinion 84. Int J Syst Evol Microbiol, 2008, 58: 1973
7 Lindström K, Young JPW. International Committee on Systematics of Prokaryotes Subcommittee on the taxonomy of Agrobacterium and Rhizobium. Int J Syst Evol Microbiol, 2009, 59: 921~922
8 Zhang XX, Sun L, Qiu FB, Jiang RB, Song W. Rheinheimera tangshanensis sp. nov., a novel rice root-associated bacterium. Int J Syst Evol Microbiol, 2008, 58: 2420~2424
9 Lane DJ. 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics. Stackebrandt E, Goodfellow M eds. Chichester, UK: Wiley, 1991
10 Gaunt MW, Turner SL, Rigottier-Gois L, Young JPW. Phylogenies of atpD and recA support the small subunit rRNA-based classification of rhizobia. Int J Syst Evol Microbiol, 2001, 51:2037~2048
11 东秀珠, 蔡妙英. 常见细菌系统鉴定手册. 科学出版社, 2001
12 Chen WX, Yan GH, Li JL. Numerical taxonomic study of fast-growing soybean rhizobia and a proposal that Rhizobium fredii be assigned to Sinorhizobium gen. nov. Int J Syst Bacteriol, 1988, 38:392~397
13 Stackebrandt E, Goebel BM. Taxonomic note: A place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol, 1994, 44: 846~849
14 Fox GE, Wisotzkey JD, Jr Jurtshuk P. How close is close: 16S ribosomal RNA sequence identity may not be sufficient to guarantee species identity. Int J Syst Bacteriol, 1992, 42: 166~170
15 Stackebrandt E, Frederiksen W, Garrity GM, Grimont PD, Kämpfer P, Maiden MCJ, Nesme X, Rosselló-Mora R, Swings J, Trüper HG, Vauterin L, Ward AC, Whitman WB. Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Bacteriol, 2002, 52: 1043~1047
16 Zeigler DR. Gene sequences useful for predicting relatedness of whole genomes in bacteria. Int J Syst Evol Microbiol, 2003, 53: 1893~1900
17 Gevers D, Cohan FM, Lawrence JG, Spratt BG, Coenye T, Feil EJ, Stackebrandt E, Van de Peer Y, Vandamme P, other authors. Re-evaluating prokaryotic species. Nat Rev Microbiol, 2005, 3: 733~739
18 林万明. 细菌分子遗传学分类鉴定法. 上海: 科学技术出版社, 1990
19 Rogel MA, Hernández-Lucas I, Kuykendall LD, Balkwill DL, Martínez-Romero E. Nitrogen-fixing nodules with Ensifer adhaerens harboring Rhizobium tropici symbiotic plasmids. Appl Enviro Microbiol, 2001, 67 (7): 3264~3268
20 Fröhlich J, Koustiane C, Kämpfer P, Rosselló-Mora R, Valens M, Berchtold M, Kuhnigk T, Hertel H, Maheshwari DK, König H. Occurrence of rhizobia in the gut of the higher termite Nasutitermes nigriceps. Syst Appl Microbiol, 2007, 30: 68~74
21 Song KQ (宋柯群), Jiang BH (姜彬慧), Jiang L (姜莉), Wen XQ (温晓倩), Lin Y (林颖). Isolation and biological characterization of phorate-toleration bacterium. J Saf & Environ (安全与环境学报), 2008, 8 (3): 78~81
22 Garbeva P, van Overbeek LS, van Vuurde JWL, Van Elsas JD. Analysis of endophytic bacterial communities of potato by plating and denaturing gradient gel electrophoresis (DGGE) of 16S rDNA based PCR fragments. Microb Ecol, 2001, 41: 369~383


国家自然科学基金项目(Nos. 30900001,30770069)、中国农业科学院农业资源与农业区划研究所中央级公益性科研院所基本科研业务费专项资金(No. 901-10)、公益性行业(农业)科研专项经费(No. 200803029-01)和北京市自然科学基金项目(No. 5092004)资助
更新日期/Last Update: 2010-12-29