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[1]张晓霞,邱服斌,孙磊,等.水稻内生粘着剑菌的分子系统发育分析[J].应用与环境生物学报,2010,16(06):779-783.[doi:10.3724/SP.J.1145.2010.00779]
 ZHANG Xiaoxia,QIU Fubin,SUN Lei,et al.Phylogenetic Analysis of Endophytic Ensifer adhaerens Isolated from Rice Roots[J].Chinese Journal of Applied & Environmental Biology,2010,16(06):779-783.[doi:10.3724/SP.J.1145.2010.00779]
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水稻内生粘着剑菌的分子系统发育分析()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
16卷
期数:
2010年06期
页码:
779-783
栏目:
研究论文
出版日期:
2010-12-25

文章信息/Info

Title:
Phylogenetic Analysis of Endophytic Ensifer adhaerens Isolated from Rice Roots
作者:
张晓霞邱服斌孙磊马晓彤姜瑞波宋未
(1中国农业科学院资源与区划研究所农业部作物营养与施肥重点实验室 北京 100081)
(2首都师范大学生命科学学院 北京 100048)
(3山西医科大学公共卫生学院 太原 030001)
(4河北大学生命科学学院河北省微生物多样性研究与应用实验室 保定 071002)
Author(s):
ZHANG Xiaoxia QIU Fubin SUN Lei MA Xiaotong JIANG Ruibo SONG Wei
(1Key Laboratory of Crop Nutrition and Fertilization, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, China)
(2College of Life Sciences, Capital Normal University, Beijing 100048, China)
(3College of Public Health, Shanxi Medical University, Taiyuan 030001, China)
关键词:
水稻内生菌粘着剑菌系统发育分析结瘤试验
Keywords:
endophytic bacterium from rice Ensifer adhaerens phylogenetic analysis nodulation test
分类号:
S182
DOI:
10.3724/SP.J.1145.2010.00779
文献标志码:
A
摘要:
通过16S rRNA基因和看家基因recA、atpD、rpoB的序列测定与分析,及(G+C) mol%测定等技术,对分离自河北省滦南县水稻根内的两株细菌进行分子系统发育学研究. 根据系统进化树构建及其同源性分析,可确定所分离的两株水稻内生细菌属于剑菌属(Ensifer)系统发育分支;供试菌株的各保守序列与粘着剑菌(E. adhaerens)模式菌种同源性最高,除recA为97.2%外,其他均为99%~100%,可以确定供试菌属于E. adhaerens;对所分离的菌株与大豆、菜豆、三叶草、苜蓿等4种豆科植物进行结瘤试验,结果显示其中一株J3-N55可与苜蓿结瘤. 野生型的E. adhaerens菌株与豆科植物结瘤的报道尚为鲜见;本文首次报道从水稻植株内分离到E. adhaerens菌株. 图4 表1 参22
Abstract:
Two Ensifer (Sinorhizobium) -like strains isolated from rice (Oryza sativa L.) roots in Luannan County, Hebei, China were investigated with a polyphasic strategy. The 16S rRNA, recA, atpD and rpoB genes were PCR amplified and sequenced, and followed by Neighbour-Joining phylogenetic analysis. The G+C content was determined by thermal denaturation method. According to the study of phylogenetics, the two strains were clustered in the genus Ensifer (Sinorhizobium), with the highest similarity to E. adhaerens ranging from 99% to 100%, except for recA gene (97.2%). To determine the nodulation ability, the nodulation tests were carried out using four different legumes: soybean (Glycine max), kidney bean (Phaseolus vulgaris), clover (Trifolium repens), and lucern (Medicago sativa). It was found that strain J3-N55 could form nodules successfully on M. sativa. To our knowledge, this is the first report about the endophytic E. adhaerens isolated from rice plant. Fig 4, Tab 1, Ref 22

参考文献/References:

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备注/Memo

备注/Memo:
国家自然科学基金项目(Nos. 30900001,30770069)、中国农业科学院农业资源与农业区划研究所中央级公益性科研院所基本科研业务费专项资金(No. 901-10)、公益性行业(农业)科研专项经费(No. 200803029-01)和北京市自然科学基金项目(No. 5092004)资助
更新日期/Last Update: 2010-12-29