|本期目录/Table of Contents|

[1]王俪鲆,张良,刘来雁,等.泸州古酿酒窖池中两株产甲烷杆菌比较研究[J].应用与环境生物学报,2010,16(06):840-844.[doi:10.3724/SP.J.1145.2010.00840]
 WANG Liping,ZHANG Liang,LIU Laiyan,et al.Comparative Study of Two Methanobacterium Strains in the Ancient Fermentation Pits of Luzhou[J].Chinese Journal of Applied & Environmental Biology,2010,16(06):840-844.[doi:10.3724/SP.J.1145.2010.00840]
点击复制

泸州古酿酒窖池中两株产甲烷杆菌比较研究()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
16卷
期数:
2010年06期
页码:
840-844
栏目:
研究论文
出版日期:
2010-12-25

文章信息/Info

Title:
Comparative Study of Two Methanobacterium Strains in the Ancient Fermentation Pits of Luzhou
作者:
王俪鲆张良刘来雁张宿义许德富刘光烨
(1中国科学院成都生物研究所 成都 610041)
(2泸州老窖股份有限公司 泸州 646000)
(3农业部沼气科学研究所,农业部能源微生物与利用重点开放实验室 成都 610041)
(4中国科学院研究生院 北京 100049)
Author(s):
WANG Liping ZHANG Liang LIU Laiyan ZHANG Suyi XU Defu LIU Guangye
(1Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China)
(2Luzhoulaojiao Co., Ltd., Luzhou 646000, Sichuan, China)
(3Biogas Institute of Ministry of Agricultural, Key Laboratory of Energy Microbiology and Application, Ministry of Agriculture, Chengdu 610041, China)
(4Graduate University of Chinese Academy of Sciences, Beijing 100049, China)
关键词:
产甲烷菌比较研究新种分离窖泥16S rDNA序列系统发育分析泸州老窖
Keywords:
methanogen Methanobacterium comparative study new strain isolation pit mud 16S rDNA sequence phylogenetic analysis Luzhoulaojiao
分类号:
Q939.97 : TQ92
DOI:
10.3724/SP.J.1145.2010.00840
文献标志码:
A
摘要:
采用厌氧操作技术,从泸州老窖古酿酒窖池窖泥中分离到两株产甲烷杆菌0372-D1和0072-D2. 0372-D1菌体形态为长杆状,略弯,两端整齐,不运动,可由多个菌体形成长链;在固体培养基中难以长出菌落,只利用H2+CO2产生甲烷. 0072-D2菌体形态为弯曲杆状,淡黄色圆形菌落,利用H2+CO2或甲酸盐作为唯一碳源生长. 两株菌最适生长温度均为35 ℃、菌株0372-D1最适生长pH为6.5~7.0,生长pH范围5.0~8.0;菌株0072-D2最适生长pH则为7.5. 在各自最适条件下培养,两株菌的最短增代时间分别为19 h和8 h. 通过形态、生理生化特征和16S rDNA序列的同源性分析,表明菌株0372-D1为产甲烷杆菌属的一个新种. 0072-D2则为甲酸甲烷杆菌(Methanobacterium formicicum)的新菌株,相似性为99%. 图6 表1 参16
Abstract:
Two strains of Methanobacterium, named as 0372-D1 and 0072-D2, were isolated from the ancient fermentation pits of Luzhoulaojiao by using the Hungate anaerobic techniques. The cells of 0372-D1 are long rod, slightly curved, both ends neatly and nonmotile, which may form long chains by some cells. However, they are difficult to form colony in the solid medium. This strain utilizes only H2+CO2 to produce methane. Strain 0072-D2 is curved in rod-shape, light yellow and forms round colonies, and it utilizes formate or H2+CO2 as its sole carbon source for growth. The optimal growth temperature of two strains was found at 35 ℃. The optimal growth pH of strain 0372-D1 was 6.5~7.0 and its growth pH ranged from 5.0 to 8.0, and the optimal growth pH of strain 0072-D2 occurred at pH 7.5. The double time was 19 h for 0372-D1 and 8 h for 0072-D2 when cultured under the optimal conditions. The result from the analysis of their morphological and physicobiochemical characteristics and homology of the 16S rDNA gene sequences demonstrated that strain 0372-D1 would be a novel species of Methanobacterium, and strain 0072-D2 belonged to M. formicicum with the highest similarity of 99% in 16S rDNA gene sequence. Fig 6, Tab 1, Ref 16

参考文献/References:

1 北原觉雄. 细菌利用工业. 平安译. 北京: 中国轻工出版社, 1956. 147~156
2 Liu GY (刘光烨), Zhao YZ (赵一章), Wu YY (吴衍庸). Isolation and characterization of Methanobacterium bryantii of Luzhou-Liqure pit mud. Microbiology (微生物学通报), 1987, 14 (3): 156~159
3 Wu YY (吴衍庸), Lu SH (卢世珩), Liu GY (刘光烨). A study of enhancing the quality of Luzhou type Qujiu by using simulataneous fermentation of caproic acid bacteria and Methane bacteria. Food & Ferment Ind (食品与发酵工业), 1990, 6: 2~6
4 Romesser JA, Wolfe RS, Mayer F. Methanogenium, a new genus of marine methanogenic bacteria, and characterization of Methanogenium cariaci sp. nov. and Methanogenium marisnigri sp. nov. Arch Microbiol, 1979, 121 (2): 147~153
5 Hungate RE. A roll-tube method for cultivation of strict anaerobes. Mthods Microbiol, 1969, 3B: 117~132
6 Balch WE, Wolfe RS. New approach to the cultivation of methanogenic bacteria: 2- mercaptoethanesulfonic acid (HS-CoM)-dependent growth of Methanobacterium ruminantium in a preesureized atmosphere. Appl Environ Microbiol, 1976, 32 (6): 781~791
7 Cuzin N, Ouattara AS, Labat M, Garcia JL. Methanobacterium congolense sp. nov., from a methanogenic fermentation of cassava peel. Intern J Syst & Evol Microbiol, 2001, 51 (2): 489~493
8 Qiu TL (仇天雷), Cheng L (承磊), Luo H (罗辉), Zhang H (张辉), Wu XL (吴晓磊), Deng Y (邓宇). Isolation and characterization of Methanogens from sedinents in Jiaozhou Bay. China Biogas (中国沼气), 2006, 25 (2): 3~10
9 Skillman LC, Evans PN, Strömpl C, Joblin KN. 16SrDNA directed PCR primers and detection of methanogens in the bovine rumen. Lett Appl Microbiol, 2006, 42: 222~228
10 Wright A-DG, Williams AJ, Winder B, Christophersen CT, Rodgers SL, Smith KD. Molecular diversity of rumen methanogens from sheep in Western Australia. Appl Environ Microbiol, 2004, 70 (3): 1263~1270
11 农业部厌氧微生物重点开放实验室. 产甲烷菌及其研究方法. 成都: 成都电子科技大学出版社, 1997. 14~15
12 Sun Z (孙征), Zhou YG (周宇光), Dong XZ (东秀珠). Characterization and phylogenetics of a new species of genus methanobacterium. Acta Microbiol Sin (微生物学报), 2001, 41 (3): 265~269
13 Boone, DR, Whitman, WB. Proposal of minimal standards for describing new taxa of methanogenic bacteria. Int Syst Bacteriol, 1988, 38: 212~219
14 Patel GB, Sprott GD, Fein JE. Isolation and characterization of Methanobacterium espanolae sp. Nov., a mesophilic, moderately acidiphilic methanogen. Int J Syst Bacteriol, 1990, 40: 12~18
15 Bräuer SL, Cadillo-Quiroz H, Yashiro E, Yavitt JB, Zinder SH. Isolation of a novel acidiphilic methanogen from an acidic peat bog. Nature, 2006, 442: 192~194
16 Bräuer SL, Yashiro E, Ueno NG, Yavitt JB, Zinder SH. Characterization of acid-tolerant H2/CO2-utilizing methanogenic enrichment cultures from and acidic peat bog in New York State. FEMS Microbiol Ecol, 2006, 57: 206~216

相似文献/References:

[1]傅霖,辛明秀.产甲烷菌的生态多样性及工业应用[J].应用与环境生物学报,2009,15(04):574.[doi:10.3724/SP.J.1145.2009.00574]
 FU Lin,XIN Mingxiu.Ecological Diversity and Industrial Application of Methanogens[J].Chinese Journal of Applied & Environmental Biology,2009,15(06):574.[doi:10.3724/SP.J.1145.2009.00574]
[2]胡亚东,袁月祥,闫志英,等.一株生长pH较宽的产甲烷菌分离与系统发育分析[J].应用与环境生物学报,2009,15(04):554.[doi:10.3724/SP.J.1145.2009.00554]
 HU Yadong,YUAN Yuexiang,YAN Zhiying,et al.Identification and Phylogenetic Analysis of a strain Methanogen with Wide Range of Growing pH[J].Chinese Journal of Applied & Environmental Biology,2009,15(06):554.[doi:10.3724/SP.J.1145.2009.00554]
[3]袁敏,张辉,胡国全.一株兼性嗜冷小甲烷粒菌的生物学特性及系统发育分析[J].应用与环境生物学报,2010,16(05):705.[doi:10.3724/SP.J.1145.2010.00705]
 YUAN Min,ZHANG Hui,HU Guoquan.Biological Characteristics and Phylogenetic Analysis of a Strain of Methanocorpusculum parvum[J].Chinese Journal of Applied & Environmental Biology,2010,16(06):705.[doi:10.3724/SP.J.1145.2010.00705]
[4]王芳,刘晓飞,刘晓风,等.产氢菌对沼气发酵的生物强化作用[J].应用与环境生物学报,2013,19(02):351.[doi:10.3724/SP.J.1145.2013.00351]
 WANG Fang,LIU Xiaofei,LIU Xiaofeng,et al.Biotechnological Intensification of Biogas Fermentation by Hydrogen Producing Bacteria[J].Chinese Journal of Applied & Environmental Biology,2013,19(06):351.[doi:10.3724/SP.J.1145.2013.00351]
[5]王珊珊,韩娅新,何环,等.煤层水中一株产甲烷菌的分离与系统发育分析[J].应用与环境生物学报,2014,20(01):123.[doi:10.3724/SP.J.1145.2014.00123]
 WANG Shanshan,HAN Yaxin,HE Huan,et al.Isolation and phylogenic analysis of a methanogen from coal bed water[J].Chinese Journal of Applied & Environmental Biology,2014,20(06):123.[doi:10.3724/SP.J.1145.2014.00123]
[6]叶飞,吴胜军,黄培,等.微氧折流反应器启动过程产甲烷菌群落结构变化特征[J].应用与环境生物学报,2014,20(03):407.[doi:10.3724/SP.J.1145.2014.08026]
 YE Fei,WU Shengjun,HUANG Pei,et al.Structure characteristics of methanogenic community produced during the startup of microaerobic baffled reactor[J].Chinese Journal of Applied & Environmental Biology,2014,20(06):407.[doi:10.3724/SP.J.1145.2014.08026]
[7]方晓瑜,李家宝,芮俊鹏,等.产甲烷生化代谢途径研究进展[J].应用与环境生物学报,2015,21(01):1.[doi:10.3724/SP.J.1145.2014.08019]
 FANG Xiaoyu,LI Jiabao,RUI Junpeng,et al.Research progress in biochemical pathways of methanogenesis[J].Chinese Journal of Applied & Environmental Biology,2015,21(06):1.[doi:10.3724/SP.J.1145.2014.08019]
[8]张小元,李香真,李家宝.微生物互营产甲烷研究进展[J].应用与环境生物学报,2016,22(01):156.[doi:10.3724/SP.J.1145.2015.07033]
 ZHANG Xiaoyuan,,et al.Microbial syntrophic methanogenesis: a review[J].Chinese Journal of Applied & Environmental Biology,2016,22(06):156.[doi:10.3724/SP.J.1145.2015.07033]
[9]王艳发,魏士平,崔鸿鹏,等.祁连山冻土区土壤活动层与冻土层中甲烷代谢微生物群落结构特征[J].应用与环境生物学报,2016,22(22卷04):592.[doi:10.3724/SP.J.1145.2016.01051]
 WANG Yanfa,WEI Shiping**,et al.Methane metabolic microbial community structure in the active layer and the permafrost layer of the Qilian permafrost, China*[J].Chinese Journal of Applied & Environmental Biology,2016,22(06):592.[doi:10.3724/SP.J.1145.2016.01051]
[10]陈颖,邱凯瑞,吴麒,等.桂林会仙岩溶湿地产甲烷菌的数量、群落组成和活性[J].应用与环境生物学报,2017,23(06):959.[doi:10.3724/SP.J.1145.2016.11043]
 CHEN Ying,**,QIU Kairui,et al.Methanogenic community structure, abundance, and activity in Huixian karst wetland, Guilin, China[J].Chinese Journal of Applied & Environmental Biology,2017,23(06):959.[doi:10.3724/SP.J.1145.2016.11043]

更新日期/Last Update: 2010-12-29