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[1]谢天文,袁月祥,闫志英,等.一株嗜酸性产纤维素酶真菌的特性及产酶条件优化[J].应用与环境生物学报,2010,16(06):863-869.[doi:10.3724/SP.J.1145.2010.00863]
 XIE Tianwen,YUAN Yuexiang,YAN Zhiying,et al.Characteristics of an Acidophilic Cellulase-producing Fungus and Optimization of Its Fermentation Condition[J].Chinese Journal of Applied & Environmental Biology,2010,16(06):863-869.[doi:10.3724/SP.J.1145.2010.00863]
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一株嗜酸性产纤维素酶真菌的特性及产酶条件优化()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
16卷
期数:
2010年06期
页码:
863-869
栏目:
研究论文
出版日期:
2010-12-25

文章信息/Info

Title:
Characteristics of an Acidophilic Cellulase-producing Fungus and Optimization of Its Fermentation Condition
作者:
谢天文袁月祥闫志英刘晓风贺蓉娜廖银章
(1中国科学院成都生物研究所 成都 610041)
(2中国科学院研究生院 北京 100049)
Author(s):
XIE Tianwen YUAN Yuexiang YAN Zhiying LIU Xiaofeng HE Rongna LIAO Yinzhang
(1Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China)
(2Graduate University of Chinese Academy of Sciences, Beijing 100049, China)
关键词:
嗜酸纤维素酶土曲霉系统发育分析响应面法最优培养条件
Keywords:
acidophilic cellulase Aspergillus terreus phylogenetic methodology RSM optimum fermentation condition
分类号:
TQ925 : Q936
DOI:
10.3724/SP.J.1145.2010.00863
文献标志码:
A
摘要:
从四川海螺沟原始森林腐土中分离到一株嗜酸性产纤维素酶的真菌X-13,其主要特点是产纤维素酶的最适pH及其纤维素酶最适反应pH均为2.0. 在PDA培养基上培养时菌落呈浅黄色至肉桂色,反面呈黄色至棕褐色,产黄色色素;菌丝体透明有隔膜,分生孢子呈球形或近球形. 根据菌株的形态特征以及ITS序列同源性和系统发育分析结果,鉴定该菌株为土曲霉(Aspergillus terreus Thom). 该菌最佳产酶培养时间为8~10 d;最适产酶温度为30 ℃,纤维素酶最适反应温度为50 ℃;最佳碳源、氮源分别为纤维素粉和硫酸铵. 通过响应面法对菌株产纤维素酶条件进行优化,使菌株X-13纤维素酶活从1.39 IU/mL提高到2.94 IU/mL,提高了111.5%. 图10 表7 参15
Abstract:
A novel acidophilic cellulase-producing fungus was isolated from the primary forest soil in Hailuogou, Sichuan, China. It was identified and named as Aspergillus terreus Thom X-13 by its morphological characters and ITS sequence phylogenetic analysis. The optimum growth pH of strain X-13 and optimum reaction pH of its cellulase were both 2.0, and its optimum growth temperature and optimum reaction temperature of its cellulase were 30 ℃ and 50 ℃, respectively. The best carbon and nitrogen sources were cellulose and ammonium sulphate, respectively. Response surface methodology (RSM) was used to optimize the submerged fermentation condition of cellulase production, and the cellulase activity of X-13 reached 2.94 IU/mL under the optimum fermentation condition, and it was increased by 111.5% compared with the initial fermentation condition. Fig 10,
Tab 7, Ref 15

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备注/Memo

备注/Memo:
国家高技术研究发展计划(863计划)项目(No. 2007AA100702)、国家科技支撑项目(Nos. 2006BAD07A02,2006BAJ04B02)、中国科学院项目(Nos. KSCXZ-XB23-07-02,KSCXZ-YW-9-008)
更新日期/Last Update: 2010-12-29