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[1]于敏,王文国,王胜华,等.外源柠檬酸对水稻铜毒害的缓解效应[J].应用与环境生物学报,2010,16(05):617-621.[doi:10.3724/SP.J.1145.2010.00617]
 YU Min,WANG Wenguo,WANG Shenghua,et al.Alleviative Effect of Exogenous Citric Acid on Rice under Copper Toxicity[J].Chinese Journal of Applied & Environmental Biology,2010,16(05):617-621.[doi:10.3724/SP.J.1145.2010.00617]
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外源柠檬酸对水稻铜毒害的缓解效应()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
16卷
期数:
2010年05期
页码:
617-621
栏目:
胁迫抗性生理及分子机制专栏
出版日期:
2010-10-25

文章信息/Info

Title:
Alleviative Effect of Exogenous Citric Acid on Rice under Copper Toxicity
文章编号:
201001015
作者:
于敏王文国王胜华陈放
(四川大学生命科学学院 成都 610064)
Author(s):
YU MinWANG WenguoWANG ShenghuaCHEN Fang
(College of Life Sciences, Sichuan University, Chengdu 610064, China)
关键词:
水稻铜毒害柠檬酸缓解效应生理特性
Keywords:
rice copper toxicity citric acid alleviative effect physiological property
分类号:
S511.01 : X17
DOI:
10.3724/SP.J.1145.2010.00617
文献标志码:
A
摘要:
以水稻(宜香4106,Oryza sativa L. cv. Yixiang 4106)种子为实验材料,研究铜(Cu)对水稻种子萌发和生长的影响,以及外施柠檬酸对铜毒害的缓解效应. 结果表明,在不同浓度Cu2+(20、50、80 μmol/L)胁迫下,水稻幼苗的芽长、胚根长、须根数和活力指数均明显下降. 当Cu2+浓度达到80 μmol/L时,与未胁迫处理相比,幼苗芽长和胚根长度抑制率分别高达23.6%和96.7%. 2 mmol/L的外源柠檬酸可缓解Cu2+的抑制作用,水稻种子萌发的活力指数提高,根系长度增长近7倍,幼苗平均每株根数增加约8.8倍,水稻幼苗叶片中丙二醛(MDA)含量降低,叶绿素含量提高41.9%,可溶性蛋白质的含量为铜胁迫处理的2.9倍,超氧化物歧化酶(SOD)、过氧化物酶(POD)的活性均显著提高. 图3 参20
Abstract:
Using rice seed (Oryza sativa L. cv. Yixiang 4106) as material to study the toxicity of Cu2+ to the seed germination and the alleviative effect of exogenous citric acid. The result showed that different Cu2+ concentrations could restrain seedling length, radicle length, amount of hair roots and vigor index. Under 80 μmol/L of Cu2+, seedling length and radicle length were inhibited by 23.6% and 96.7%, respectively. The toxicity of Cu2+ was relieved to some extent after adding 2.0 mmol/L citric acid, increasing the vigor index. The radicle length and hair roots were increased by 7 times and 8.8 times, respectively. Physiological and biochemical changes occurred, and chlorophyll and soluble protein contents increased by 41.9% and 2.9 times, superoxide dismutase (SOD) and peroxidase (POD) activities increase by 12.3% and 88.6%, malondialdehyde (MDA) content declined by 47.3%. Fig 3, Ref 20

参考文献/References:

1 Lin YZ (林义章), Xu L (徐磊). Physiological toxicity of copper pollution to higher plant. Chin J Eco-Agric (中国生态农业学报), 2007, 15 (1): 201~204
2 Cao ZH, Hu ZY. Copper contamination in paddy soils irrigated with wastewater. Chemosphere, 2000, 41: 3~ 6
3 Chen YX, He YF, Luo YM, Yu YL, Lin Q, Wong MH. Physiological mechanism of plant roots exposed to cadmium. Chemosphere, 2003, 50: 789~ 793
4 Murphy AS, Eisinger WR, Shaff JE, Kochian LV, Taiz L. Early copper-induced leakage of K+ from Arabidopsis seedlings is mediated by ion channels and coupled to citrate efflux. Plant Physiol, 1999, 121 (4): 1375~1382
5 Yang H, Yang ZM, Zhou LX, Wong JW. Ability of Agrogyron elongatum to accumulate the single metal of cadmium, copper, nickel and lead and root exudation of organic acids. J Environ Sci China, 2001,13 (3): 368~375
6 Jin TT (金婷婷), Liu P (刘鹏), Sun T (孙婷), Liu CS (刘春生), Jin HH (金海华). Localized accumulation of aluminum in soybean (Glycine max Merrill) root tips under exogenous citric acid. Soybean Sci (大豆科学), 2008, 27 (4): 581~587
7 Juan M, Verenice RR, José L, Liu HE. Aluminum tolerance in transgenic plants by alteration of citrate synthesis. Science, 1997, 276 (5318): 1566~1568
8 Han F, Shan XQ, Zhang SZ, Bei W, Owens G. Enhanced cadmium accumulation in maize roots-the impact of organic acids. Plant & Soil, 2006, 289 (1~2): 355~368
9 廖宗永, 林纲, 李云武, 张杰, 杨从金, 江青山. 优质香型杂交稻宜香4106的选育及栽培制种技术. 中国稻米, 2008 (1): 36~37
10 Li HS (李合生), Sun Q (孙群), Zhao SJ (赵世杰), Zhang WH (章文华). Plant Physiological Biochemical Experiment. Beijing, China : Higher Education Press (北京: 高等教育出版社), 2006. 260~261, 167~169, 184~185
11 Zhang ZL (张志良), Zhai WJ (翟伟菁). Plant Physiological Experiment Instruction. Beijing, China: Higher Education Press (北京: 高等教育出版社), 2004: 274~277
12 Shao LX (邵邻相), Huang BZ (黄伯钟), Ding SJ (丁淑静). Effects of zinc manganese ferrous and copper on growth and superoxide dismutase activity of rice seedlings. Seed (种子), 2001 (6): 16~19
13 Lu HL (卢豪良), Yan ZL (严重玲). Exudation of low-molecular-weight-organic acids by Kandelia candel (L) Druce roots and implication on heavy metal bioavailability in mangrove sediments. Acta Ecol Sin (生态学报), 2007, 27 (10): 4173~4181
14 Feng XY (冯效毅), Zhang YF (张宇峰), Liu CY (刘春阳). Effect of citric acid on adsorption of Cu by wheat. Poll Control Technol, 2007, 20 (4): 28~30
15 Fan XT (范雪涛), Ma DW (马丹炜), Xiang S (向莎), Yang XJ (杨晓娇), Zhong LW (钟良文), Chen L (陈兰), He W (何微). Change in antioxidant enzymes of Galinsoga parviflora Cav. under environmental stresses. Chin J Appl Environ Biol (应用与环境生物学报), 2008, 14 (5): 616~619
16 Torres MA, Dangl JL. Functions of the respiratory burst oxidase inbiotic interactions, abiotic stress and development. Curr Opin Plant Biol, 2005, 8: 397 ~403
17 Zhang CM (张春梅), Zou ZR (邹志荣), Zhang ZX (张志新), Huang Z (黄志). Effects of exogenous spermidine on reactive oxygen levels and antioxidative system of tomato seedling under polyethlene glycol stress. Chin J Appl Environ Biol (应用与环境生物学报), 2009, 15 ( 3 ): 301~307
18 Patsikka E, Kairavuo M. Sersen F, Aro E, Tyystjarvi E. Excess copper predisposes photosystemⅡ to photoinhibition in vivo by out competing iron and causing decrease in leaf chlorophyⅡ. Plant Physiol, 2002, 129: 1359 ~1367
19 Zhang LJ (张露洁), Lan LQ (兰利琼), Qing RW (卿人韦), Ye HX (叶华勋), Fu HL (傅华龙). Study on the impact of a several heavy metals ion to the chlorophyll content of tissue cultured Dioscorea zingiberensis. J Sichuan Univ Nat Sci Ed (四川大学学报自然科学版), 2006, 43 (1): 200~205
20 Xing SZ ( 邢素芝), Wang JF (汪建飞), Cai D (蔡丹). Effects of citrate and malate on the seed germination and memberane permeability of pakchoi. Chin Agric Sci Bull (中国农学通报), 2007, 23 (9): 312~316

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备注/Memo

备注/Memo:
农业部转基因重大专项计划(No. 2009ZX08009-072B)资助 Supported by the Key Transgene Technology Project of Ministry of Agriculture of China (No. 2009ZX08009-072B)
更新日期/Last Update: 2010-10-25