|本期目录/Table of Contents|

[1]付雯,张晓勇,周金燕,等.固定化枯草芽孢杆菌发酵生产捷安肽素[J].应用与环境生物学报,2009,15(02):230-234.[doi:10.3724/SP.J.1145.2009.00230]
 FU Wen,ZHANG Xiaoyong,et al.Jiean-peptide Prodution by Immobilized Cell Fermentation of Bacillus subtilis[J].Chinese Journal of Applied & Environmental Biology,2009,15(02):230-234.[doi:10.3724/SP.J.1145.2009.00230]
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固定化枯草芽孢杆菌发酵生产捷安肽素()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
15卷
期数:
2009年02期
页码:
230-234
栏目:
研究论文
出版日期:
2009-03-15

文章信息/Info

Title:
Jiean-peptide Prodution by Immobilized Cell Fermentation of Bacillus subtilis
作者:
付雯张晓勇周金燕钟娟谭红
(1中国科学院成都生物研究所 成都 610041; 2中国科学院研究生院 北京 100049)
Author(s):
FU Wen1 2 ZHANG Xiaoyong1 ZHOU Jinyan1 ZHONG Juan1 & TAN Hong1
(1Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China)
(2Graduate University of Chinese Academy of Sciences, Beijing 100049, China)
关键词:
固定化细胞捷安肽素枯草芽孢杆菌木屑粘稠剂响应面方法
Keywords:
immobilized cell Jiean-peptide Bacillus subtilis wood chip adhesive agent response surface methodology
分类号:
TQ920.1 : S476.8
DOI:
10.3724/SP.J.1145.2009.00230
文献标志码:
A
摘要:
采用吸附固定化细胞技术发酵生产捷安肽素. 首先对7种不同的吸附性固体载体进行筛选,发现木屑作为载体效果最好;然后通过正交优化实验和单因素实验确定载体木屑的最佳条件为:粒度0.5~0.6 mm,添加浓度10 g L-1发酵液. 为避免吸附法固定的菌体细胞从载体上脱落,尝试在木屑固定化细胞体系中加入粘稠剂以增强吸附性能,运用单因素实验确定了粘稠剂的种类和浓度,即为15 g L-1海藻酸钠. 最后应用响应面方法优化固定化细胞发酵培养基及培养条件,结果表明:接种量为10%、黄豆粉水解液总氮浓度为1.7 g L-1发酵液、葡萄糖浓度为33.7 g L-1发酵液时捷安肽素的生物效价最大,达到7 282.08 U,比非固定化细胞培养提高了53.9%. 图3 表7 参17
Abstract:
The paper described a novel method for adsorption and application of immobilized cells for Jiean-peptide production. Seven absorptive solids were chosen as the candidate carriers for immobilization of Bacillus subtilis cells and among them wood chips were identified as the best. The optimal conditions of wood chips as B. subtilis cells’ carriers were 0.5~0.6 mm in diameter and 10 g L-1 in fermentation broth, which were determined through single factor and orthogonal experiments. In order to avoid the detachment of the immobilized B. subtilis cells from the carriers, different adhesive agents were added into the wood chips to increase its absorption, and 15 g L-1 sodium alginate gel was found to be the best by single factor experiment. Then the optimal fermentation media and conditions were obtained by response surface methodology: 10% inocula, 1.7 g L-1 total nitrogen of soymeal hydrolysate and 33.7 g L-1 glucose in fermentation broth. Under these conditions, the biological potency of Jiean-peptide peaked as high as 7 282.08 U, which was 53.9% higher than that in carrier-free liquid cultivation. Fig 3, Tab 7, Ref 17

参考文献/References:

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14 Zhong J (钟娟). Study and improvement of a strain producing Jiean-peptide: [Master’s Degree Dissertation]. Chengdu, China (成都): Chengdu Institute of Biology, Chinese Academy of Sciences (中国科学院成都生物研究所), 2003
15 Xu GY (徐广宇), Luo JM (骆健美), Yang DS (杨德山), Wang M (王敏). Optimization of Natamycin fermentation culture media and conditions. J Microbiol (微生物学杂志), 2007, 27 (4): 73~78
16 Jiang YX (江元翔), Gao SH (高淑红), Chen CH (陈长华). Optimization of medium com ponents for adenosine production by Bacillus subtilis using response surface design. J East China Univ Sci & Technol (华东理工大学学报), 2005, 31 (3): 309~313
17 Yang WQ (杨文清), Hu J (胡晋), Wu B (吴斌), Zhang S (张胜), Song WJ (宋文坚). Combining response surface analysis and principal component analysis to optimize the combination of GA3 concentration in film coating and storage time of coated seed in cucumber. J Biomathmatics (生物数学学报), 2007, 22 (2): 323~328

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备注/Memo

备注/Memo:
中国科学院知识创新工程重要方向项目(No. KSCX2-YW-G-039-2)资助 Supported by the Knowledge Innovation Project of the Chinese Academy of Sciences (No. KSCX2-YW-G-039-2)
更新日期/Last Update: 2009-05-05