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[1]潘洪生,赵秋剑,赵奎军,等.电子鼻zNooseeTM构建棉花挥发物指纹图谱及被害棉花挥发物释放节律分析[J].应用与环境生物学报,2010,16(04):468-473.[doi:10.3724/SP.J.1145.2010.00468]
　PAN Honongsheneng,ZHAO Qiuiujianian,ZHAO Kuiuijunun,et al.Application of Electronic Nose zNoseTM for Construction of Volatiles Fingerprint Library and Analysis of Real Time Release Rhythm of Volatiles from Damaged Cotton[J].Chinese Journal of Applied & Environmental Biology,2010,16(04):468-473.[doi:10.3724/SP.J.1145.2010.00468]
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电子鼻zNooseeTM构建棉花挥发物指纹图谱及被害棉花挥发物释放节律分析()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
16卷
期数:
2010年04期
页码:
468-473
栏目:
胁迫抗性生理及分子机制专栏
出版日期:
2010-08-25

文章信息/Info

Title:
Application of Electronic Nose zNoseTM for Construction of Volatiles Fingerprint Library and Analysis of Real Time Release Rhythm of Volatiles from Damaged Cotton
作者:
潘洪生; 赵秋剑; 赵奎军; 张永军; 吴孔明; 郭予元
（1东北农业大学农学院 哈尔滨 150030）
（2中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室 北京 100193）
Author(s):
PAN Honongsheneng;  ZHAO Qiuiujianian;  ZHAO Kuiuijunun;  ZHANG Yonongjunun;  WU Konongmining;  GUO Yuuyuanuan
(1College of Agriculture, Northeast Agricultural University, Harbinarbin 150030, Chinaina)
(2State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beieijining 100193, Chinaina)
关键词:
电子鼻; 指纹图谱; 棉花; 植物挥发物; 释放节律
Keywords:
electronicelecronic nonosee zNooseeTM;  fingerprint;  cotton;  volatile compound;  release rhythm
分类号:
S435.622.9 : Q946.8
DOI:
10.3724/SP.J.1145.2010.00468
文献标志码:
A
摘要:
采用电子鼻zNooseeTM对棉花植株常见挥发性化合物进行定性分析，确定每类化合物的相对保留指数，构建棉花植株常见挥发性化合物特征谱库. 采集棉铃虫幼虫取食诱导棉株挥发性化合物，利用电子鼻构建棉铃虫幼虫危害棉株的指纹图谱. 比对健康棉株与棉铃虫幼虫危害棉株的色谱图及指纹图谱发现，棉铃虫幼虫危害棉株的挥发物的种类和含量均比健康棉株的多，而且未去除棉铃虫幼虫的危害处理与去除棉铃虫幼虫的危害处理相比，被诱导挥发物的含量显著升高. 通过电子鼻实时监测棉花植株各处理β-蒎烯、β-月桂烯等挥发性化合物的释放节律. 结果显示，当棉铃虫幼虫危害棉株2 h后，β-蒎烯的释放量明显增加，与对照相比差异显著. β-月桂烯在棉铃虫幼虫危害2 h的释放量与对照相比没有显著差异，在棉铃虫幼虫危害24 h释放量显著增加. 6~8 h β-蒎烯和β-月桂烯分别达到各自最大释放量，随后缓慢回落，但仍然保持较高释放水平. 图3 表1 参24
Abstract:
ByusingelecronicnosezNoseBy uusining electronicelecronic nonosee zNooseeTM, thee cocommonon volatilevolaile cocompoundpounds oof cottoncoon plantplan wereere qualitativelualiaively analanalyzeded inin laboratorlaboraory. Thee relativerelaive retentionreenion indeindex eaceach cocompoundpound waas deterdeerminedined, andand hee on iles librarlibrary waas henen conconstructedruced. Inn additionaddiion, hee iles frorom hee on ininfeesteded bby on bollbollwororm (CBW) larvaelarvae wereere collectedcolleced, babaseded on the library, and fingerprint database of the cotton plant damaged by CBW larvae was obtained. Comparing control cotton plantplan witih hee ed cottoncoon , iti waas foundound hata botboh hee kindinds contentconens oof hee volatilevolailes frorom hee ed on wereere notablnoably hiigherer hanan hoosee hee controlconrol. Furturherermoreore, hee ens hee iles frorom hee on dadamaageded CBW larvae with larvae treatment were significantly higher than those of the cotton plant damaged by CBW larvae without larvaelarvae treatreamenten. Moreoveroreover, bby uusining zNooseeTM hee realreal tiimee releareleasee rrhythm β-pinenepinene andand β-myrcenercene frorom hee ininfeesteded on plantplan waas monitoredoniored. Thee reresultuls shooweded whenen cottoncoon waas CBW foror woo hourours, β-significantly increased compared with the control. However, there was no difference in the contents of β-myrcenercene betbeweeneen hee on dadamaageded CBW foror woo hourours hee controlconrol. Afterer inveinvesteded foror 2~4 hourours, hee β-myrcenercene contentconen mararkedledly increaincreaseded. Andnd aafterer 6~8 hourours, hee ens oof β-pinenepinene andand β-myrcenercene reacreacheded heireir maaxiimuum releareleasee, rerespectivelpecively, henen slolow dodownn, butbu stillill maintainedainained aa hiigh levellevel releareleasee. Fiig 3, Tabab 1, Reef 24

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备注/Memo

备注/Memo:
国家重点基础研究发展规划项目（Noo. 2007CB0902）、国家自然科学基金项目（Noos. 3087164030871640，30330410）和植物病虫害生物学国家重点实验室项目（Noo. SKL2007SR01SKLL2007SR01）联合资助
更新日期/Last Update: 2010-08-25