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[1]邱宗波,张晋豫,孙立,等.增强UV-B辐射下矮牵牛表型变异生理及RAPD多态性变化[J].应用与环境生物学报,2010,16(03):323-327.[doi:10.3724/SP.J.1145.2010.00323]
 QIU Zongbo,ZHANG Jinyu,SUN Li,et al.Physiological and RAPD Variation of Petunia hybrida Exposed to Enhanced Ultraviolet-B Radiation[J].Chinese Journal of Applied & Environmental Biology,2010,16(03):323-327.[doi:10.3724/SP.J.1145.2010.00323]
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增强UV-B辐射下矮牵牛表型变异生理及RAPD多态性变化()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
16卷
期数:
2010年03期
页码:
323-327
栏目:
胁迫抗性生理及分子机制专栏
出版日期:
2010-06-25

文章信息/Info

Title:
Physiological and RAPD Variation of Petunia hybrida Exposed to Enhanced Ultraviolet-B Radiation
作者:
邱宗波张晋豫孙立岳明
(1河南师范大学生命科学学院 新乡 453007)
(2西北大学生命科学学院 西安 710069)
Author(s):
QIU Zongbo ZHANG Jinyu SUN Li YUE Ming
(1College of Life Sciences, Henan Normal University, Xinxiang 453007, Henan, China)
(2College of Life Sciences, Northwest University, Xi’an 710069, China)
关键词:
UV-B辐射矮牵牛表型变异HPLCRAPD
Keywords:
UV-B radiation Petunia hybrida Phenotype variation HPLC RAPD
分类号:
Q682 : Q945.78
DOI:
10.3724/SP.J.1145.2010.00323
文献标志码:
A
摘要:
采用增强UV-B辐射矮牵牛,研究增强UV-B辐射下矮牵牛发生变异的生理生化机制. 结果表明,变异矮牵牛丙二醛(Malondialdehyde,MDA)含量显著低于对照矮牵牛(P<0.01),而黄酮类化合物含量显著高于对照矮牵牛(P<0.01),高效液相色谱图的最高主峰保留时间分别为11.818 min(对照矮牵牛)和13.783 min(变异矮牵牛). 同时应用随机扩增多态性DNA(Random amplified polymorphic DNA,RAPD)技术对矮牵牛进行检测分析,发现引物在对照矮牵牛及变异矮牵牛之间扩增出差异性多态条带,表明增强UV-B辐照引起矮牵牛基因组DNA发生变异. 图5 参28
Abstract:
The physiological and RAPD variations and phenotype change of Petunia hybrida caused by exposure to enhanced UV-B radiation with its seedlings were studied. The results showed that the MDA content in the variant plants of P. hybrida was markedly lower than that in the control plants, while the flavonoids content of the variant plants was markedly higher than that of the control. The retention time of HPLC high peak was 11.818 and 13.783 min for the control and variant plants, respectively. RAPD (Random amplified polymorphic DNA) variation of the control and variant plants induced by the enhanced UV-B radiation suggested that random primers would have been amplified with different DNA polymorphism bands. Consequently, the enhanced UV-B radiation could cause mutation of genome DNA in P. hybrida. Fig 5, Ref 28

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备注/Memo

备注/Memo:
国家自然科学基金项目(No. 30670366)、河南省生物化学与分子生物学重点学科和河南师范大学青年科学基金项目(No. 2008qk15)资助 Supported by the National Natural Sciences Foundation of China (No. 30670366), Key Subject of Biochemistry and Molecular Biology of Henan Province and the Youth Science Foundation of Henan Normal University (No. 2008qk15)
更新日期/Last Update: 2010-06-23