|本期目录/Table of Contents|

[1]袁敏,张辉,胡国全.一株兼性嗜冷小甲烷粒菌的生物学特性及系统发育分析[J].应用与环境生物学报,2010,16(05):705-709.[doi:10.3724/SP.J.1145.2010.00705]
 YUAN Min,ZHANG Hui,HU Guoquan.Biological Characteristics and Phylogenetic Analysis of a Strain of Methanocorpusculum parvum[J].Chinese Journal of Applied & Environmental Biology,2010,16(05):705-709.[doi:10.3724/SP.J.1145.2010.00705]
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一株兼性嗜冷小甲烷粒菌的生物学特性及系统发育分析()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
16卷
期数:
2010年05期
页码:
705-709
栏目:
研究论文
出版日期:
2010-10-25

文章信息/Info

Title:
Biological Characteristics and Phylogenetic Analysis of a Strain of Methanocorpusculum parvum
文章编号:
200911017
作者:
袁敏张辉胡国全
(1农业部沼气科学研究所 成都 610041)
(2农业部能源微生物与利用重点开放实验室 成都 610041)
Author(s):
YUAN MinZHANG HuiHU Guoquan
(1Biogas Institute of the Ministry of Agriculture, Chengdu 610041, China)
(2Key Laboratory of Energy Microbiology and Application of the Ministry of Agriculture, Chengdu 610041, China)
关键词:
小甲烷粒菌产甲烷菌16S rDNA系统发育分析
Keywords:
Methanocorpusculum parvum methanogen 16S rDNA phylogenetic analysis
分类号:
Q939
DOI:
10.3724/SP.J.1145.2010.00705
文献标志码:
A
摘要:
采用改进的亨盖特(Hungate)厌氧技术,从西藏林芝厌氧消化系统中分离到一株产甲烷菌菌株LZ-6. 该菌株为革兰氏阴性,不运动,球形,直径约0.3~0.6 μm. 该菌株利用H2/CO2,微利用甲酸生长,不利用乙酸、甲醇、甲醇/H2、三甲胺、甲胺,最适生长pH为6.8~7.2,最适生长温度25 ℃,最适Na +浓度0.2 mol/L. 菌株LZ-6 的16S rRNA基因序列与小甲烷粒菌(Methanocorpusculum parvum)相似性为99%. 生理、形态结构特征等生物学特性研究表明此株产甲烷古菌为兼性嗜冷产甲烷古菌. 图3 表2 参17
Abstract:
A strain of methanogen LZ-6 was isolated from an anaerobic digester in Tibet, China by Hungate anaerobic technique. Of the strain, the cells were Gram negative, nonmotile, cocci of 0.3~0.6 μm in diameter. The catabolic substrates used included H2/CO2 and formate, but no acetate, methanol, methanol/H2 or methylated amines. Its growth was observed in a temperature range between 4 ℃ and 35 ℃ with an optimum at 25 ℃. The NaCl optimum for growth was 0.2 mol/L, and the fastest growth occurred within a pH range of 6. 8~7.2. Strain LZ-6 was 99% similar in 16S rRNA gene sequence to Methanocorpusculum parvum. Its physiological, morphological and structural characteristics reveal that the strain is a eurypsychrophile methanogen. Fig 3, Tab 2, Ref 17

参考文献/References:

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备注/Memo

备注/Memo:
国家高技术研究发展计划(863计划,No. 2007AA100705)、四川省重点科技攻关课题(No. 2006Z07-004-02)和科研院所技术开发专项资金(No. 2009EG134272) Supported by the National High Technology Research and Development Program of China (No.2007AA100705), the Key Project of Department of Science and Technology of Sichuan, China (No.2006Z07-004-02) and the Special Funds for Scientific Research and Technological Development of Institutes of China (No.2006Z07-004-02)
更新日期/Last Update: 2010-10-25