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 XIE Tianwen,LIU Xiaofeng,YUAN Yuexiang,et al.Progress in Research of Inducers and Regulation Mechanism of Fungal Cellulase[J].Chinese Journal of Applied & Environmental Biology,2010,16(03):440-444.[doi:10.3724/SP.J.1145.2010.00440]





Progress in Research of Inducers and Regulation Mechanism of Fungal Cellulase
(1中国科学院成都生物研究所 成都 610041)
(2中国科学院研究生院 北京 100049)
XIE Tianwen LIU Xiaofeng YUAN Yuexiang YAN Zhiying HE Rongna  LIAO Yinzhang
(1Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China)
(2Graduate University of Chinese Academy of Sciences, Beijing 100049, China)
fungi cellulase inducer induction repression expression regulation
Q949.320.6 : Q556
木质纤维素原料作为一种储量丰富、价格低廉的可再生资源,在生物燃料以及相关高附加值产品领域的应用已成为一个研究热点. 纤维素酶是木质纤维素原料资源化利用过程中的关键酶,但从自然中筛选的纤维素降解菌株酶活较低,因而制约了木质纤维素原料的资源化利用. 本文综述了真菌产纤维素酶的诱导物及纤维素酶表达调控机理研究的主要进展,着重讨论了纤维素、纤维二糖、槐糖、龙胆二糖等诱导物对真菌产纤维素酶的诱导作用以及葡萄糖等代谢产物的抑制作用,并阐述了真菌纤维素酶诱导表达调控的机理以及纤维素酶基因表达激活子(ACE)、木聚糖酶转录激活因子(Xyr1)、内切葡聚糖苷酶激活元件(CAE)、分解代谢物抑制蛋白(CRE)等调控因子的研究进展. 提出通过对纤维素酶合成代谢进行调控来高效合成纤维素酶,为提高纤维素酶的活性和纤维素酶工业化应用提供参考. 参51
Lignocellulosic material is a kind of abundant and less costly renewable natural resource, which is becoming a focal point of research for the productions of bio-fuels and other relative products with high values. Cellulase is the key enzyme of utilizing the lignocellulosic material. At present, the cellulase activity is very low, which restricts its industrial application. This review presents the discussions on the inductive effects of cellulose, cellobiose, sophorose and gentiobiose, and the repression effect of some metabolites including glucose on fungal cellulase. The regulation mechanism of fungal cellulase expression and the cellulase expression factors as activators of cellulase gene expression (ACE), xylanase regulator Xyr1, cbh2-activating element (CAE) and catabolite repressor protein (CRE) are also introduced. This review indicates that the cellulase can be produced effectively, which would largely satisfy its industrial production by regulating its metabolic synthesis artificially. Ref 51


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国家高技术研究发展计划(863计划,No. 2007AA100702)、中国科学院项目(Nos. KSCXZ-XB23-07-02,KSCXZ-YW-9-008)和国家科技支撑项目(Nos. 2006BAD07A02,2006BAJ04B02)资助 Supported by the National High Technology Research and Development Program of China (No. 2007AA100702), the Projects of the Chinese Academy of Sciences (Nos. KSCXZ-XB23-07-02, KSCXZ-YW-9-008), and the Key Projects of the National Science & Technology Pillar Program of China (Nos. 2006BAD07A02, 2006BAJ04B02)
更新日期/Last Update: 2010-06-23