|本期目录/Table of Contents|

[1]谢天文,刘晓风,袁月祥,等.真菌产纤维素酶的诱导物及其调控机理研究进展[J].应用与环境生物学报,2010,16(03):440-444.[doi:10.3724/SP.J.1145.2010.00440]
 XIE Tianwen,LIU Xiaofeng,YUAN Yuexiang,et al.Progress in Research of Inducers and Regulation Mechanism of Fungal Cellulase[J].Chinese Journal of Applied & Environmental Biology,2010,16(03):440-444.[doi:10.3724/SP.J.1145.2010.00440]
点击复制

真菌产纤维素酶的诱导物及其调控机理研究进展()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
16卷
期数:
2010年03期
页码:
440-444
栏目:
综述
出版日期:
2010-06-25

文章信息/Info

Title:
Progress in Research of Inducers and Regulation Mechanism of Fungal Cellulase
作者:
谢天文刘晓风袁月祥闫志英贺蓉娜廖银章
(1中国科学院成都生物研究所 成都 610041)
(2中国科学院研究生院 北京 100049)
Author(s):
XIE Tianwen LIU Xiaofeng YUAN Yuexiang YAN Zhiying HE Rongna  LIAO Yinzhang
(1Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China)
(2Graduate University of Chinese Academy of Sciences, Beijing 100049, China)
关键词:
真菌纤维素酶诱导物诱导阻遏表达调控
Keywords:
fungi cellulase inducer induction repression expression regulation
分类号:
Q949.320.6 : Q556
DOI:
10.3724/SP.J.1145.2010.00440
文献标志码:
A
摘要:
木质纤维素原料作为一种储量丰富、价格低廉的可再生资源,在生物燃料以及相关高附加值产品领域的应用已成为一个研究热点. 纤维素酶是木质纤维素原料资源化利用过程中的关键酶,但从自然中筛选的纤维素降解菌株酶活较低,因而制约了木质纤维素原料的资源化利用. 本文综述了真菌产纤维素酶的诱导物及纤维素酶表达调控机理研究的主要进展,着重讨论了纤维素、纤维二糖、槐糖、龙胆二糖等诱导物对真菌产纤维素酶的诱导作用以及葡萄糖等代谢产物的抑制作用,并阐述了真菌纤维素酶诱导表达调控的机理以及纤维素酶基因表达激活子(ACE)、木聚糖酶转录激活因子(Xyr1)、内切葡聚糖苷酶激活元件(CAE)、分解代谢物抑制蛋白(CRE)等调控因子的研究进展. 提出通过对纤维素酶合成代谢进行调控来高效合成纤维素酶,为提高纤维素酶的活性和纤维素酶工业化应用提供参考. 参51
Abstract:
Lignocellulosic material is a kind of abundant and less costly renewable natural resource, which is becoming a focal point of research for the productions of bio-fuels and other relative products with high values. Cellulase is the key enzyme of utilizing the lignocellulosic material. At present, the cellulase activity is very low, which restricts its industrial application. This review presents the discussions on the inductive effects of cellulose, cellobiose, sophorose and gentiobiose, and the repression effect of some metabolites including glucose on fungal cellulase. The regulation mechanism of fungal cellulase expression and the cellulase expression factors as activators of cellulase gene expression (ACE), xylanase regulator Xyr1, cbh2-activating element (CAE) and catabolite repressor protein (CRE) are also introduced. This review indicates that the cellulase can be produced effectively, which would largely satisfy its industrial production by regulating its metabolic synthesis artificially. Ref 51

参考文献/References:

1 陈洪章. 纤维素生物技术. 北京: 化学工业出版社, 2005. 46~50
2 Levinson HS, Reese ET. Enzymatic hydrolysis of soluble cellulose derivatives as measured by changes in viscosity. J GenPhysiol, 1950, 33 (5): 601~628
3 Watanabe H, Takase A, Tokuda G, Yamada A, Lo N. Symbiotic “archaezoa” of the primitive termite Mastotermes darwiniensis still play a role in cellulase production. Eukaryotic Cell, 2006, 5 (9): 1571~1576
4 Mandels M, Reese ET. Induction of cellulase in Trichoderma viride as influenced by carbon sources and metals. J Bacteriol, 1957, 73 (2): 269~278
5 Loginova LG, Tashpulatov Z. The thermophilic fungus Aspergillus fumigatus forming active cellulase. Mikrobiologiia, 1965, 34 (2): 258~64
6 Lindvall S, Eriksson AFV. Studies on a new cellulase preparation from Penicillium.2 Properties and action upon different substrates. J Pharm & Pharmacol, 1959, 11 (12): 756~763
7 Ikeda Y, Hayashi H, Okuda N, Park EY. Efficient cellulase production by the filamentous fungus Acremonium cellulolyticus. Biotechnol Progr, 2007, 23 (2): 333~338
8 Kruszewska J, Palamarczyk G, CP Kubicek. Stimulation of exoprotein secretion by choline and tween-80 in Trichoderma reesei QM-9414 correlates with increased activities of dolichol phosphate mannose synthase. J Gen Microbiol, 1990, 136: 1293~1298
9 Kubicek CP, Messner R, Gruber F, Mach RL, Kubicekpranz EM. The Trichoderma cellulase regulatory puzzle: From the interior life of a secretory fugus. Enzyme & Microb Technol, 1993, 15 (2): 90~99
10 Mandels M, Reese ET. Induction of cellulase in fungal by cellobiose. J Bacteriol, 1960, 79 (6): 816~826
11 Wang XF (王晓芳), Xu XS (徐旭士), Wu M (吴敏), Yang YN (杨亚南). Induction and regulation of cellulase formation in two trains of fungi by different carbon sources. Chin J Appl Environ Biol (应用与环境生物学报), 2002, 8 (6): 653~657
12 Fritscher C, Messner R, Kubicek CP. Cellobiose metabolism and cellobiohydrolase-I biosynthesis by Trichoderma reesei. ExpMycol, 1990, 14 (4): 405~415
13 Iyayi CB, Bruchmann EE, Kubicek CP. Induction of cellulase formation of cellulase formation in Trichoderma reesei by cellobiono-1,5-lacton. Arch Microbiol, 1989, 151 (4): 326~330
14 Szakmary K, Wotawa A, Kubicek CP. Origin of oxidized cellulose degradation products and mechanism of their promotion of cellobiohydrolase- I biosynthesis in Trichoderma reesi. J Gen Microbiol, 1991, 137: 2873~2878
15 Loewenberg JR, Chapman CM. Sophorose metabolism and cellulase inducion in Trichoderma. Arch Microbiol, 1977, 113 (1~2): 61~64
16 Sternberg D, Mandels GR. Induction of cellulolytic enzymes in Trichoderma reesei by sophorose. J Bacteriol, 1979, 139 (3): 761~769
17 Huang ZY (黄振艳). Study on the production of cellulase soluble inducer and its fundamental application in cellulase production: [Marster Degree Disertation]. Hangzhou, China: Zhejiang University (杭州: 浙江大学), 2008
18 Lo CM, Ju LK. Sophorolpids Induced cellulase production in co-cultures of Hypocrea jecorina Rut C30 and Candida bombicola. Enzyme & Microb Technol, 2009, 44 (2): 107~111
19 Chaudhuri BK, Sahai V. Production of cellulase enzyme from lactose in batch and continuous cultures by a partially constitutive strain of Trichoderma-Reesei. Enzyme & Microb Technol, 1993, 15 (6): 513~518
20 Sehnem NT, De Bittencourt LR, Camassola M, Dillon AJP. Cellulase production by Penicillium echinulatum on lactose. Appl Microbiol & Biotechnol, 2006, 72 (1): 163~167
21 Fang X, Yano S, Inoue H, Sawayama S. Lactose enhances cellulase production by the filamentous fungus Acremonium cellulolyticus. J Biosci & Bioeng, 2008, 106 (2): 115~120
22 Ai YC (艾云灿), Meng FM (孟繁梅), Gao PJ (高培基), Kubicek Christian P. Basis of specificity of induction and repression by cellobiose on cellulase biosynthesis in fungi. Acta Sci Naturalium Univ Sunyatseni (中山大学学报自然科学版), 2000, 39 (3): 47, 57, 67, 77
23 Sahoo DK, Mishra S, Bisaria VS. Influence of L-sorbose on growth and enzyme-synthesis of Trichoderma reesei C-5. J GenMicrobiol, 1986, 132: 2761~2766
24 Karaffa L, Fekete E, Gamauf C, Szentirmai A, Kubicek CP, Seiboth B. D-galactose induces cellulase gene expression in Hypocrea jecorina at low growth rates. Microbiology-Sgm, 2006, 152: 1507~1514
25 Nogawa M, Goto M, Okada H, Morikawa Y. L-Sorbose induces cellulase gene transcription in the cellulolytic fungus Trichoderma reesei. CurrGenet, 2001, 38 (6): 329~334
26 Ai BL (艾斌凌), Wang YQ (王义强), Chen JN (陈介南). Enhancement of bran to cellulase production by Trichoderma reesei Rut C-30. Biomass Chem Eng (生物质化学工程), 2009, 43 (2): 27~33
27 Hrmova M, Petrakova E, Biely P. Induction of cellulose- and xylan-degrading enzyme systems in Aspergillus terreus by homo- and heterodisaccharides composed of glucose and xylose. J Gen Microbiol, 1991, 137 (3): 541~547
28 Mandels M, Reese ET, Parrish FW. Sophorose as an inducer of cellulase in Trichoderma viride. J Bacteriol, 1962, 83 (2): 400~408
29 Vaheri MP, Vaheri MEO, Kauppinen VS. Formation and release of cellulolytic enzymes during growth of Trichoderma reesei on cellobiose and glycerol. Eur J Appl Microbiol & Biotechnol, 1979, 8 (1~2): 73~80
30 Elgogary S, Leite A, Crivellaro O, Eveleigh DE, Eldorry H. Mechanism by which cellulose triggers cellobiohydrolase-I gene expression in Trichoderma reesei. Proc Nat Acad Sci USA, 1989, 86 (16): 6138~6141
31 CarleUrioste JC, EscobarVera J, ElGogary S, HenriqueSilva F, Torigoi E, Crivellaro O, HerreraEstrella A, ElDorry H. Cellulase induction in Trichoderma reesei by cellulose requires its own basal expression. J Biol Chem, 1997, 272 (15): 10169~10174
32 Zhu YS (朱雨生). Induction and regulation of cellulase formation in Trichoderma Ⅳ: Changes in regulatory mechanisms of cellulase synthesis of two mutants with high cellulase yields—a preliminary analysis of the mechanism of increase in cellulase production. Acta Photophysiol Sin (植物生理与分子生物学学报), 1978, 18 (2): 402~431
33 Claeyssens M, Vantilbeurgh H. Studies of the cellulolytic system of the filamentous fungus Trichoderma reesei QM-9414 substrate specificity and transfer activity of endoglucanase-I. Biochem J, 1990, 270 (1): 251~256
34 Kubicek CP. Involvement of a conidial endoglucanase and a plasma-membrane-bound beta-glucosidase in the Induction of endogucanase synthesis by cellulose in Trichoderma reesei. J Gen Microbiol, 1987, 133: 1481~1487
35 Mach RL, Seiboth B, Myasnikov A, Gonzalez R, Strauss J, Harkki AM, Kubicek CP. The bgl1 gene of Trichoderma reesei QM-9414 encodes an extra-cellular cellulose inducible beta-glucosidase involved in cellulase induction by Sophorose. Mol Microbiol, 1995, 16 (4): 687~697
36 Nisizawa T, Suzuki H, Nakayama M, Nisizawa K. Inductive formation of cellulase by sophorose in Trichoderma viride. J Biochem, 1971, 70 (3): 375~385
37 Messner R, Kubicek CP. Carbon source control of cellobiohydrolase-I and cellobiohydrolase-II formation by Trichoderma reesei. Appl & Environ Microbiol, 1991, 57 (3): 630~635
38 Huang Y (黄艳), Qin Y (覃拥灵), Ling M (凌敏), Li W (李玮), Liang ZQ (梁智群). Study on cellulases production by Trichoderma Knoningii with the inducement of different carbon sources. China Brew (中国发酵), 2008 (15): 41~44
39 Zhu YS (朱雨生), Tan C (谭常). Induction and regulation of cellulase formation in TrichodermaⅡ: Induction of cellulase by sophorose in washed mycelia of T. pseudokoningii EA_3-867 and its catabolite repression. Acta Photophysiol Sin (植物生理与分子生物学学报), 1978, 18(1): 302~331
40 Nisizawa T, Suzuki H. Catabolite repression of cellulase formation in Trichoderma viride. J Biochem, 1972, 71 (6): 999~1007
41 Sun XY (孙宪昀), Qu YB (曲音波), Liu ZY (刘自勇). Progress in research of lignocellulose-degrading enzymes from Penicillium. Chin J Appl Environ Biol (应用与环境生物学报), 2007, 13 (5): 736~740
42 Saloheimo A, Aro N, Ilmen M, Penttila M. Isolation of the ace1 gene encoding a Cys (2)-His (2) transcription factor involved in regulation of activity of the cellulase promoter cbh1 of Trichoderma reesei. J Biol Chem, 2000, 275 (8): 5817~5825
43 Rauscher R, Wurleitner E, Wacenovsky C, Aro N, Stricker AR, Zeilinger S, Kubicek CP, Penttila M, Mach RL. Transcriptional regulation of xyn1, encoding xylanase I, in Hypocrea jecorina. Eukaryotic Cell, 2006, 5 (3): 447~456
44 Ling M (凌敏), Qin YL (覃拥灵), Li N (李楠), Liang ZQ (梁智群). Cloning and functional analysis of two regulators involved in regulation of cellulase formation in Trichoderma koningii. Chin J Biotech (生物工程学报), 2008, 24 (10): 1808~1812
45 Zeilinger S, Mach RL, Kubicek CP. Two adjacent protein binding motifs in the cbh2 (cellobiohydrolase II-encoding) promoter of the fungus Hypocrea jecorina (Trichoderma reesei) cooperate in the induction by cellulose. J Biol Chem, 1998, 273 (51): 34463~34471
46 Ilmen M, Onnela ML, Klemsdal S, Penttila M. Functional analysis of the cellobiohydrolase I promoter of the filamentous fungus Trichoderma reesei. Mol & Gen Genet, 1996, 253 (3): 303~314
47 Kubicek CP, Harman GE. Trichoderma and Gliocladium. London: Taylor & Francis Ltd, 1998. 75~94
48 Schmoll M, Kubicek CP. Regulation of Trichoderma cellulase formation: Lessons in molecular biology from an industrial fungus: A review. Acta Microbiol et Immunol Hungarica, 2003, 50 (2~3): 125~145
49 Gremel G, Dorrer M, Schmoll M. Sulphur metabolism and cellulase gene expression are connected processes in the filamentous fungus Hypocrea jecorina (anamorph Trichoderma reesei). BMC Microbiol, 2008, 8 (174): 1~18
50 Schmoll M, Schuster A, Silva RD, Kubicek CP. The G-Alpha protein GNA3 of Hypocrea jecorina (anamorph Trichoderma reesei) regulates cellulase gene expression in the presence of light. Eukaryotic Cell, 2009, 8 (3): 410~420
51 Rahman Z, Shida Y, Furukawa T, Suzuki Y, Okada H, Ogasawara W , Morikawa Y. Application of Trichoderma reesei cellulase and xylanase promoters through homologous recombination for enhanced production of extracellular beta-glucosidase I. Biosci Biotechnol & Biochem, 2009, 73 (5): 1083~1089

相似文献/References:

[1]邱栋梁,刘星辉,郭素枝.模拟酸雨对龙眼幼果纤维素酶活性和内源激素含量的影响[J].应用与环境生物学报,2004,10(01):35.
 QIU Dongliang,et al..Effects of simulated acid rain on cellulase activity and contents of endogenous hormone in young fruit of longan[J].Chinese Journal of Applied & Environmental Biology,2004,10(03):35.
[2]杨革,陈洪章,刘艳.添加剂对绿色木霉JQF-04纤维素酶热稳定性的影响[英文][J].应用与环境生物学报,2009,15(01):134.[doi:10.3724/SP.J.1145.2009.00134]
 YANG Ge,CHEN Hongzhang,LIU Yan.Effect of Additives on Thermostability of Trichoderma viride JQF-04 Cellulase[J].Chinese Journal of Applied & Environmental Biology,2009,15(03):134.[doi:10.3724/SP.J.1145.2009.00134]
[3]王慧,郑小伟,王宾香,等.真菌对染料的脱色研究进展[J].应用与环境生物学报,2009,15(01):147.[doi:10.3724/SP.J.1145.2009.00147]
 WANG Hui,ZHENG Xiaowei,WANG Binxiang,et al.Advance in Decolorization of Dye by Fungi[J].Chinese Journal of Applied & Environmental Biology,2009,15(03):147.[doi:10.3724/SP.J.1145.2009.00147]
[4]张亚波,刘连盟,徐荣燕,等.嗜热子囊菌光孢变种内切葡聚糖酶I基因在毕赤酵母中的表达及部分酶学性质[J].应用与环境生物学报,2009,15(03):419.[doi:10.3724/SP.J.1145.2009.00419]
 ZHANG Yabo,LIU Lianmeng,XU Rongyan,et al.Expression of Thermoascus aurantiacus var. levisporus Endo-β-glucanase I Gene in Pichia pastoris and Anylysis of Enzymic Properties[J].Chinese Journal of Applied & Environmental Biology,2009,15(03):419.[doi:10.3724/SP.J.1145.2009.00419]
[5]魏艳红,熊鹰,袁永泽,等.纤维素酶产生菌HS-F9的筛选鉴定和产酶条件优化[J].应用与环境生物学报,2010,16(02):274.[doi:10.3724/SP.J.1145.2010.00274]
 WEI Yanhong,XIONG Ying,YUAN Yongze,et al.Screening and Identification of a Cellulase-producing Fungus and Optimization of Its Fermentation Conditions[J].Chinese Journal of Applied & Environmental Biology,2010,16(03):274.[doi:10.3724/SP.J.1145.2010.00274]
[6]谢天文,袁月祥,闫志英,等.一株嗜酸性产纤维素酶真菌的特性及产酶条件优化[J].应用与环境生物学报,2010,16(06):863.[doi:10.3724/SP.J.1145.2010.00863]
 XIE Tianwen,YUAN Yuexiang,YAN Zhiying,et al.Characteristics of an Acidophilic Cellulase-producing Fungus and Optimization of Its Fermentation Condition[J].Chinese Journal of Applied & Environmental Biology,2010,16(03):863.[doi:10.3724/SP.J.1145.2010.00863]
[7]许利霞,徐荣,赵焕玉,等.黑胸散白蚁纤维素酶的体外酶学特性[J].应用与环境生物学报,2012,18(01):70.[doi:10.3724/SP.J.1145.2012.00070]
 XU Lixia,XU Rong,ZHAO Huanyu,et al.In vitro Characteristics of the Cellulases from Reticulitermes chinensis Snyder[J].Chinese Journal of Applied & Environmental Biology,2012,18(03):70.[doi:10.3724/SP.J.1145.2012.00070]
[8]黄玉红,靳艳玲,赵云,等.鲜甘薯发酵生产燃料乙醇中的降粘工艺[J].应用与环境生物学报,2012,18(04):661.[doi:10.3724/SP.J.1145.2012.00661]
 HUANG Yuhong,JIN Yanling,ZHAO Yun,et al.Viscosity Reduction During Fuel Ethanol Production by Fresh Sweet Potato Fermentation[J].Chinese Journal of Applied & Environmental Biology,2012,18(03):661.[doi:10.3724/SP.J.1145.2012.00661]
[9]胡蓉,张姝,吴建伟,等.家蝇纤维素酶的检测及酶活性与其发育的关系[J].应用与环境生物学报,2013,19(03):444.[doi:10.3724/SP.J.1145.2013.00444]
 HU Rong,ZHANG Shu,WU Jianwei,et al.Detection of Cellulases and the Relationship Between Enzyme Activity and Development of Musca domestica[J].Chinese Journal of Applied & Environmental Biology,2013,19(03):444.[doi:10.3724/SP.J.1145.2013.00444]
[10]龚志婷,赖泳红,周艳梅,等.云南保山温泉产纤维素酶高温菌多样性研究[J].应用与环境生物学报,2013,19(04):699.[doi:10.3724/SP.J.1145.2013.00699]
 GONG Zhiting,LAI Yonghong,ZHOU Yanmei,et al.Diversity of Cellulose-producing Thermophilic Bacteria in the Baoshan Hot Spring[J].Chinese Journal of Applied & Environmental Biology,2013,19(03):699.[doi:10.3724/SP.J.1145.2013.00699]

备注/Memo

备注/Memo:
国家高技术研究发展计划(863计划,No. 2007AA100702)、中国科学院项目(Nos. KSCXZ-XB23-07-02,KSCXZ-YW-9-008)和国家科技支撑项目(Nos. 2006BAD07A02,2006BAJ04B02)资助 Supported by the National High Technology Research and Development Program of China (No. 2007AA100702), the Projects of the Chinese Academy of Sciences (Nos. KSCXZ-XB23-07-02, KSCXZ-YW-9-008), and the Key Projects of the National Science & Technology Pillar Program of China (Nos. 2006BAD07A02, 2006BAJ04B02)
更新日期/Last Update: 2010-06-23