|本期目录/Table of Contents|

[1]孟珍,尤晓颜,姜成英,等.系列硫氧化还原酶(SOR)的同源建模[J].应用与环境生物学报,2010,16(03):424-428.[doi:10.3724/SP.J.1145.2010.00424]
 MENG Zhen,YOU Xiaoyan,JIANG Chengying,et al.Homology Modeling for Sulfur Oxygenase/Reductase[J].Chinese Journal of Applied & Environmental Biology,2010,16(03):424-428.[doi:10.3724/SP.J.1145.2010.00424]
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系列硫氧化还原酶(SOR)的同源建模()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
16卷
期数:
2010年03期
页码:
424-428
栏目:
技术与方法
出版日期:
2010-06-25

文章信息/Info

Title:
Homology Modeling for Sulfur Oxygenase/Reductase
作者:
孟珍尤晓颜姜成英马俊才
(1中国科学院微生物研究所微生物资源前期开发国家重点实验室 北京 100101)
(2中国科学院计算机网络信息中心科学数据中心 北京 100190)
Author(s):
MENG Zhen YOU Xiaoyan JIANG Chengying MA Juncai
(1State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China)
(2Scientific Data Center, Computer Network Information Center, Chinese Academy of Sciences, Beijing 100190, China)
关键词:
同源建模模型合理性评估硫氧化还原酶
Keywords:
homology modeling evaluation of models sulfur oxygenase/reductase
分类号:
Q554
DOI:
10.3724/SP.J.1145.2010.00424
文献标志码:
A
摘要:
硫氧化还原酶(Sulfur oxygenase/reductase,SOR)在嗜热酸古菌硫氧化代谢中具有关键性作用,其催化机理和分子工程等都得到广泛关注. 对SOR所涉及的亚基进行分子同源建模研究,并评估其可用性,通过 1) 序列相似性搜索,2) 序列和结构比对,3) 初始建模,4) 分析和评估,5) 精修和优化获得未知三维结构SOR的三维模型. 同时讨论了此类蛋白质结构建模、评估策略、特征数据提取等. 图3 表4 参19
Abstract:
Sulfur oxygenase/reductase (SOR) plays a key role in oxidative metabolism of sulfur acid by thermophilic archaea. Its catalytic mechanism and molecular engineering are concerned widely. In this paper, the modeling and evaluation of the subunits of SORs were processed. The steps of homology modeling are as follows: 1) Search of protein sequence similarity; 2) alignment of sequence and structure ; 3) initial modeling; 4) simulation of refinement and molecular dynamics (MD); and 5) evaluation of the optimized model. This paper also discusses the modeling, evaluation and characterized data for SORs.
Fig 3, Tab 4, Ref 19

参考文献/References:

1 Emmel T, Sand W, Konig WA, Bock E. Evidence for the existence of a sulfur oxygenase in Sulfolobus brierleyi. J Gen Microbiol, 1986, 132: 3415~3420
2 He ZG, Li YQ, Zhou PJ, Liu SJ. Cloning and heterologous expression of a sulfur oxygenase/reductase gene from the thermoacidophilic archaeon Acidianus sp. S5 in Escherichia coli. FEMS Microbiol Lett, 2000, 193 (2): 217~221
3 He ZG, Zhong HF, Li YQ. Acidianus tengchongensis sp. nov., a new species of acidothermophilic archaeon isolated from an acidothermal spring. Curr Microbiol, 2004, 48 (2): 159~163
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6 Kletzin A. Molecular characterization of the sor gene, which encodes the sulfur oxygenase/reductase of the thermoacidophilic Archaeum Desulfurolobus ambivalens. J Bacteriol, 1992, 174 (18): 5854~5859
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19 Zheng ZL, Zou ZY, Liu ZG, Tsai KC, Liu AF, Zou GL. Construction of a 3D model of nattokinase, a novel fibrinolytic enzyme from Bacillus natto: A novel nucleophilic catalytic mechanism for nattokinase. J Mol Graphics Modell, 2005, 23 (4): 373~380

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备注/Memo

备注/Memo:
中国科学院知识创新工程信息化建设专项科学数据库及其应用系统(No. INF105-SDB-1-13)和工业生物技术战略研究与知识环境建设项目(No. KSCX2-YW-G-028)资助 Supported by the Knowledge Innovation Programs of Chinese Academy of Sciences for the Scientific Database and Its Application System Construction (No. INF105-SDB-1-13), and the Industrial Biotechnology Strategic Research and Knowledge Environment Construction (No. KSCX2-YW-G-028)
更新日期/Last Update: 2010-06-23