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[1]张晓霞,马晓彤,曹卫东,等.紫云英根瘤菌的系统发育多样性[J].应用与环境生物学报,2010,16(03):380-384.[doi:10.3724/SP.J.1145.2010.00380]
 ZHANG Xiaoxia,MA Xiaotong,CAO Weidong,et al.Phylogenetic Diversity of Rhizobial Bacteria Isolated from Astragalus sinicus[J].Chinese Journal of Applied & Environmental Biology,2010,16(03):380-384.[doi:10.3724/SP.J.1145.2010.00380]
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紫云英根瘤菌的系统发育多样性()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
16卷
期数:
2010年03期
页码:
380-384
栏目:
研究论文
出版日期:
2010-06-25

文章信息/Info

Title:
Phylogenetic Diversity of Rhizobial Bacteria Isolated from Astragalus sinicus
作者:
张晓霞马晓彤曹卫东韦善君蔡建辉姜瑞波
(1中国农业科学院农业资源与农业区划研究所中国农业微生物菌种保藏中心 北京 100081)
(2中央民族大学生命与环境科学学院 北京 100081)
Author(s):
ZHANG Xiaoxia MA Xiaotong CAO Weidong WEI Shanjun CAI Jianhui JIANG Ruibo
(1Agricultural Culture Collection of China, Institute of Agricultural, Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China)
(2College of Life and Environment Sciences, Central University for Nationalities, Beijing 100081, China)
关键词:
紫云英根瘤菌16S rDNA系统发育多样性γ-变形菌纲
Keywords:
Astragalus sinicus rhizobia 16S rRNA gene phylogenetics diversity γ-proteobacteria
分类号:
Q939.114.03
DOI:
10.3724/SP.J.1145.2010.00380
文献标志码:
A
摘要:
在筛选根瘤菌与不同紫云英品种高效结瘤的基础上,通过16S rRNA基因序列分析,对13株共生结瘤效果好的菌种进行了系统发育研究. 结果表明,13株菌属于3个主要聚类群,分别与Mesorhizobium、Agrobacterium和Stenotrophomonas亲缘关系最为接近,种类多样性较为丰富;其中编号为ACCC13065株的菌株与S. rhizophila 16S rDNA序列相似性为100%,位于γ-变形菌纲,这是除α-变形亚纲、β-变形亚纲外所发现的一类新的根瘤菌类型. 图4 表3 参28
Abstract:
Taxonomic and phylogenetic characteristics of 13 rhizobial strains were studied by 16S rRNA gene sequence comparisons based on during a study of screening high symbiotic matching combinations of rhizobia and Astragalus sinicus varieties. The results showed that the 13 strains could be divided into 3 clusters, belonging to three genera Mesorhizobium, Agrobacterium and Stenotrophomona, respectively. The results also indicated that rhizobia species diversity and phylogenetic diversity were abundant in the A. sinicus nodules.The 16S rRNA gene sequence of strain ACCC13065 was 100% similar to type strain of S. rhizophila, within γ-proteobacteria. It was the first time to report the legume-nodulating γ-proteobacteria besides α- and β-rhizobia. Fig 4, Tab 3, ref 28

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备注/Memo

备注/Memo:
公益性行业(农业)科研专项经费(No.200803029-01)、中央级公益性科研院所基本科研业务费专项资金(No. 2009-10)和国家自然科学基金项目(No. 30900001)资助 Supported by the Special Scientific Research Fund for Commonweal Agricultural Industry of China (No. 200803029-01), the Special Foundation for Central Academies of China (No. 2009-10) and the National Natural Science Foundation of China (No. 30900001)
更新日期/Last Update: 2010-06-23