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[1]郭艳丽,张培玉,于德爽,等.一株轻度嗜盐反硝化菌的分离鉴定及特性[J].应用与环境生物学报,2010,16(03):394-398.[doi:10.3724/SP.J.1145.2010.00394]
 GUO Yanli,ZHANG Peiyu,YU Deshuang,et al.Isolation, Identification and Characteristics of a Slightly Halophilic Denitrifying Bacterium[J].Chinese Journal of Applied & Environmental Biology,2010,16(03):394-398.[doi:10.3724/SP.J.1145.2010.00394]
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一株轻度嗜盐反硝化菌的分离鉴定及特性()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
16卷
期数:
2010年03期
页码:
394-398
栏目:
研究论文
出版日期:
2010-06-25

文章信息/Info

Title:
Isolation, Identification and Characteristics of a Slightly Halophilic Denitrifying Bacterium
作者:
郭艳丽张培玉于德爽曲洋郭沙沙成广勇
(1青岛大学环境科学与工程系 青岛 266071)
(2中海油山东化学工程有限责任公司 济南 250013)
Author(s):
GUO Yanli ZHANG Peiyu YU Deshuang QU Yang GUO Shasha CHENG Guangyong
(1Department of Environmental Science and Engineering, Qingdao University, Qingdao 266071, Shandong, China)
(2Shandong Chemical Engineering Co., Ltd., China National Offshore Oil Corporation, Jinan 250013, Shandong, China )
关键词:
嗜盐菌好氧反硝化菌系统发育分析高盐废水生物降解
Keywords:
halophilic bacterium aerobic denitrifying bacterium phylogenetic analysis high-salinity wasterwater biodegradation
分类号:
X172
DOI:
10.3724/SP.J.1145.2010.00394
文献标志码:
A
摘要:
从处理高盐度废水的成熟活性污泥中分离筛选得到1株轻度嗜盐反硝化细菌YL-1. 通过对该菌株的形态观察、生理生化实验以及16S rDNA基因序列分析,确定该菌株为迪茨氏菌(Dietzia sp.). 盐度影响实验表明,该菌株能在盐度为0~10%的培养液中生长,盐度为3%时D600 nm达到最大,为2.002;当初始硝态氮浓度为120 mg/L左右时,DO值对该菌株的反硝化效果几乎没有影响,NOX--N去除率均在90%以上,表明该菌为好氧反硝化菌;适于在碱性环境中生长,最适pH为7.5~8.5;能利用乙酸钠、蔗糖、葡萄糖、柠檬酸钠、丁二酸钠为碳源进行反硝化,以蔗糖为碳源时反硝化效果最好,NOX--N去除率达到99.8%;在25~30 ℃的温度范围内反硝化效果较好,30 ℃时菌株的NOX--N去除率超过90%. 该菌在一定盐度下具有较好的反硝化作用,能为高盐度废水的生物处理提供一定的参考价值. 图7 参15
Abstract:
A slightly halophilic denitrifying bacterium, named as YL-1, was screened from the activated sludge treating high-salinity wastewater. According to its morphological, physiological and biochemical characteristics, and the analysis of 16S rRNA gene sequence, strain YL-1 was identified as Dietzia sp. The study of the effect of salinity on its growth showed that the strain could survive at certain salinity and the suitable salinity ranged from 0 to 10%. The OD value reached the maximum 2.002 when the salinity was 3%. The nitrogen removal rate was not affected by dissolved oxygen value and could exceed 90% when the concentration of nitrate was about 120 mg/L. The nitrogen removal rate exceeded 90% when the rotation speed was 50~200 rounds per minute. The alkaline environment was suitable for its growth and the optimal pH range was from 7.5 to 8.5. Strain YL-1 could use a variety of carbon sources for denitrification, such as acetate, sucrose, glucose, sodium citrate and sodium succinate. However, sucrose was found the most suitable carbon source, and it got the nitrogen removal reaching 99.8%. The strain could perform denitrification when the temperature ranged from 25 ℃ to 30 ℃, and the nitrogen removal rate exceeded 90% at 30 ℃. Fig 7, Ref 15

参考文献/References:

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相似文献/References:

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备注/Memo

备注/Memo:
国家自然科学基金项目(Nos. 50678085, 50878107)和青岛大学优秀研究生学位论文培育项目(YSPY2009014)资助 Supported by the National Natural Science Foundation of China (Nos. 50678085, 50878107) and the Innovation Program of Graduate Education of Qingdao University, China (YSPY2009014)
更新日期/Last Update: 2010-06-23