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[1]魏艳红,熊鹰,袁永泽,等.纤维素酶产生菌HS-F9的筛选鉴定和产酶条件优化[J].应用与环境生物学报,2010,16(02):274-278.[doi:10.3724/SP.J.1145.2010.00274]
　WEI Yanhong,XIONG Ying,YUAN Yongze,et al.Screening and Identification of a Cellulase-producing Fungus and Optimization of Its Fermentation Conditions[J].Chinese Journal of Applied & Environmental Biology,2010,16(02):274-278.[doi:10.3724/SP.J.1145.2010.00274]
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纤维素酶产生菌HS-F9的筛选鉴定和产酶条件优化()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
16卷
期数:
2010年02期
页码:
274-278
栏目:
研究论文
出版日期:
2010-04-25

文章信息/Info

Title:
Screening and Identification of a Cellulase-producing Fungus and Optimization of Its Fermentation Conditions
文章编号:
200904033
作者:
魏艳红; 熊鹰; 袁永泽; 郑扬忠; 胡柳如; 张华; 李风; 杨江科; 闫云君; 刘德立
（1华中师范大学生命科学学院遗传调控与整合生物学重点实验室 武汉 430079）
（2华中科技大学生命科学与技术学院 武汉 430074）
Author(s):
WEI Yanhong; XIONG Ying; YUAN Yongze; ZHENG Yangzhong; HU Liuru; ZHANG Hua; LI Feng; YANG Jangke; YAN Yunjun; LIU Deli
(1Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Huazhong Normal University, Wuhan 430079, China)
(2College of Life Sciences and Technology, Huazhong University of Science and Technology, Wuhan 430049, China)
关键词:
纤维素酶; 菌株筛选; 产酶条件优化; 酶活性
Keywords:
cellulase;  strain screening;  optimized condition for cellulase production;  enzyme activity
分类号:
TQ925 : Q936
DOI:
10.3724/SP.J.1145.2010.00274
文献标志码:
A
摘要:
为了选育高产纤维素酶菌株，通过刚果红鉴别培养基以及滤纸条崩解实验测定，从牛粪堆肥中筛选到一株产纤维素酶的真菌HS-F9，根据菌株形态特性和18S rRNA基因序列分析，初步鉴定该菌为绿色木霉（Trichoderma viride）. 利用液体发酵培养产生纤维素酶，研究了碳源、氮源、培养时间、培养温度、培养基起始pH、接种量对菌株HS-F9产酶的影响. 结果表明：产EG、CBH和FPA的最适碳源均为CMC-Na；EG和CBH在以蛋白胨为唯一氮源时酶活最高，FPase则在以黄豆粉为唯一氮源时酶活最高；产生EG、CBH和FPA的最适温度分别为30 ℃、30 ℃、33 ℃；最适起始pH为3.0、3.0和4.0；EG和FPase的最适接种量为2%，CBH最适接种量达到了8%；培养时间均以5~6 d为宜. 在最适条件下培养，该菌株EG、CBH和FPase的酶活分别达到了5 275.3 U/mL、8 502.1 U/mL和3 619.1 U/mL，是未优化前的1.42、1.35和1.32倍. 图6 表2 参23
Abstract:
In order to breed high-yield strains for cellulase, a fungus (HS-F9) with high cellulase activity was isolated from cow dung through congo red identification medium and the collapse of filter, and was identified as Trichoderma viride by analyzing its morphology and 18S rRNA gene sequences. Different conditions including carbon source, nitrogen source, incubating time, growth temperature, initial pH and inoculation quantity for the production of cellulase were studied with liquid-state fermentation. The optimum cultural conditions of strain HS-F9 for the cellulase production were as follows: The optimum carbon source for producing EG (endo-β-1,4-glucanases), CBH (exo-β-1,4-glucanases or cellobiohy-drolase) and FPase (filter paper enzyme) was CMC-Na; the optimum nitrogen source was peptone for EG and CBH, but soy flour for FPase; the optimum temperatures for EG, CBH and FPase were 30 ℃, 30 ℃ and 33 ℃, and the optimum initial pH values were 3.0, 3.0 and 4.0, respectively; the optimum inoculation quantity for EG and FPA was 2%, while CBH was 8%; and the appropriate culture time ranged from 5 to 6 days. Under the optimum conditions, the enzyme activities of EG, CBH and FPA were up to 5 275.3 U/mL, 8 502.1 U/mL and 3 619.1U/mL, which were 1.42,1.35 and 1.32 times higher than those under un-optimized conditions, respectively. Fig 6, Tab 2, Ref 23

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备注/Memo

备注/Memo:
科技部“863”项目（No. 2007AA05Z417）、教育部博士点基金项目（No. 20060511002）和湖北省科技攻关项目（Nos. 2007AA301C26，2007AA201C50）资助 Supported by the High-tech R & D Program of China (863 Program, No. 2007AA05Z417), the Ph.D. Program Foundation of Ministry of Education of China (No. 20060511002) and the Key Sci & Tech Program of Hubei, China (Nos. 2007AA301C26, 2007AA201C50)
更新日期/Last Update: 2010-04-20