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[1]姜玮瑜,代平礼,张永军,等.转Bt-cry1Ac基因棉花对意大利蜜蜂肠道细菌群落的影响[J].应用与环境生物学报,2010,16(02):211-215.[doi:10.3724/SP.J.1145.2010.00211]
　JIANG Weiyu,DAI Pingli,ZHANG Yongjun,et al.Effect of Transgenic Cotton with cry1Ac Gene on Intestinal Bacterial Community of Apis mellifera ligustica[J].Chinese Journal of Applied & Environmental Biology,2010,16(02):211-215.[doi:10.3724/SP.J.1145.2010.00211]
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转Bt-cry1Ac基因棉花对意大利蜜蜂肠道细菌群落的影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
16卷
期数:
2010年02期
页码:
211-215
栏目:
研究论文
出版日期:
2010-04-25

文章信息/Info

Title:
Effect of Transgenic Cotton with cry1Ac Gene on Intestinal Bacterial Community of Apis mellifera ligustica
文章编号:
200904029
作者:
姜玮瑜; 代平礼; 张永军; 周婷; 林毅; 束长龙; 张杰
（1中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室 北京 100193）
（2华侨大学化工学院 厦门 361021）
（3中国农业科学院蜜蜂研究所农业部授粉昆虫生物学重点开放实验室 北京 100093）
Author(s):
JIANG Weiyu; DAI Pingli; ZHANG Yongjun; ZHOU Ting; LIN Yi; SHU Changlong; ZHANG Jie
(1State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China)
(2College of Chemical Engineering, Huaqiao University, Xiamen 361021, Fujian, China)
(3Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture, Institute of Apicultural Research,
Chinese Academy of Agricultural Sciences, Beijing 100093, China)
关键词:
转基因棉花; 蜜蜂; 肠道细菌; 基因水平转移; 变性梯度凝胶电泳
Keywords:
transgenic cotton;  honeybee;  intestinal microorganism;  gene horizontal transfer;  DGGE
分类号:
Q788 : S5035.3
DOI:
10.3724/SP.J.1145.2010.00211
文献标志码:
A
摘要:
采用基于16S rDNA的变性梯度凝胶电泳（DGGE）的方法，研究了转Bt cry1Ac基因棉花SGK321对益虫——意大利蜜蜂（Apis mellifera ligustica）肠道细菌群落的影响. 分别用转基因/非转基因棉花花粉和高浓度外源DNA饲喂蜜蜂7 d，通过DGGE分析经不同处理的蜜蜂肠道细菌群落组成的差异，同时用PCR检测是否发生外源基因的横向转移. 结果显示，各个处理组DGGE图谱中的条带数目在11.3~15.0之间，Shannon’s指数在0.87~1.05之间，相似性系数介于68.3%~84.7%，不同处理组肠道细菌组成差异不明显，用PCR的方法也没有检测到外源基因的横向转移. 因此初步判定转基因棉花SGK321短期内对蜜蜂细菌群落尚无不利影响. 图2 表3 参22
Abstract:
The effect of transgenic cotton SGK321 with cry1Ac gene on bacterial community structures in honeybee (Apis mellifera ligustica) intestines was studied by denaturing gradient gel electrophoresis (DGGE) based on sequencing of 16S rDNA. The honeybees were fed with the transgenic cotton pollens, non-transgenic cotton pollen and foreign DNA of high concentration for seven days, respectively. The intestinal bacterial diversity of each treatment was analyzed by DGGE and the possibility of a horizontal gene transfer was detected by polymerase chain reaction (PCR). The DGGE profile showed that the number of bands of all treatments ranged between 11.3~15.0 and Shannon’s index between 0.87~1.05. The similarity (Dice) coefficient among the treatments ranged from 68.3% to 84.7%. These indices showed that different diets could not change the bacterial diversity in the honeybee intestines. At the same time, gene transfer was not detected. It was concluded that the transgenic cotton had no detrimental effects on the microbial diversity in honeybee intestines in short period of time. Fig 2, Tab 3, Ref 22

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备注/Memo

备注/Memo:
国家重大基础研究计划项目（“973”项目，No. 2007CB109203）资助 Supported by the “973” Program of China (No. 2007CB109203)
更新日期/Last Update: 2010-04-20