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[1]王丽,雷娟,向文良,等.一株产紫色素放线菌的鉴定及其色素特性研究[J].应用与环境生物学报,2009,15(01):139-142.[doi:10.3724/SP.J.1145.2009.00139]
　WANG Li,LEI Juan,XIANG Wenliang,et al.Identification and Characterization of Purple Pigment-producing Actinomycete Strain[J].Chinese Journal of Applied & Environmental Biology,2009,15(01):139-142.[doi:10.3724/SP.J.1145.2009.00139]
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一株产紫色素放线菌的鉴定及其色素特性研究()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
15卷
期数:
2009年01期
页码:
139-142
栏目:
研究论文
出版日期:
2009-02-25

文章信息/Info

Title:
Identification and Characterization of Purple Pigment-producing Actinomycete Strain
作者:
王丽; 雷娟; 向文良; 蒋思远; 孙建瑞; 杨志荣
四川大学生命科学学院 成都 610064
Author(s):
WANG Li;  LEI Juan;  XIANG Wenliang;  JIANG Siyuan;  SUN Jianrui;  YANG Zhirong
College of Life Sciences, Sichuan University, Chengdu 610064, China
关键词:
比基尼链霉菌; 鉴定; 色价; 16S rRNA; 紫色素
Keywords:
Streptomyces bikiniensis;  identification;  color value;  16S rRNA sequence;  purple pigment
分类号:
Q939.9
DOI:
10.3724/SP.J.1145.2009.00139
文献标志码:
A
摘要:
从河南碱性土壤样品中筛选到一株命名为LK-178的放线菌菌株，在高氏合成培养基上其气生菌丝灰白，产
可溶性紫色素. 镜检观察孢子链长直、孢子圆柱形，通过形态、生理生化特征初步确定为链霉菌. 16S rRNA序列分析
显示，该菌基因序列与比基尼链霉菌(Streptomyces bikiniensis) AB208713基因序列有99.0%的最高相似性. 用Neighborjoining (NJ)等构建系统发育树，并用Bootstraping法对其评价，将菌株LK-178归属于比基尼链霉菌(S. bikiniensis). 抑菌试验表明，该菌具有抗革兰氏阳性、革兰氏阴性细菌和真菌活性. 用紫外可见分光光度计和化学分析确定该色素性质
稳定，产量较高，总色价相对单位较高，达到147 U/mL，产量达到13.4 g/L. 图3 表3 参18
Abstract:
An actinomycete, named strain LK-178, was isolated from an alkaline soil sample in Henan, China. It produced
grayish-white aerial mycelium and purple diffusible pigment on Gause’s synthetic agar. Microscopic observation
revealed that it produced straight chain of cylindrical spores. It was primarily identified as genus Streptomyces according
to its morphological, physiological and biochemical characteristics. The 16S rRNA gene sequence of strain LK-178
shared the highest similarity (99.0%) with that of S. bikiniensis AB208713. The phylogenetic tree of strain LK-178 was
derived with NJ and analyzed with bootstraping. The result demonstrated that it belonged to S. bikiniensis. The test on
antimicrobial activities showed that it had ability against the Gram-positive, Gram-negative bacteria and fungi. The UVVIS
spectrometry and chemical analysis revealed that the pigment producted by this strain was stable, total relative
color value of the pigment was as high as 147 U/mL and total pigment yield reached 13.4 g/L. Fig 3, Tab 3, Ref 18

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备注/Memo

备注/Memo:
四川省微生物资源共享平台项目资助
更新日期/Last Update: 2009-03-05