|本期目录/Table of Contents|

[1]郭英,刘栋,赵蕾.生防枯草芽孢杆菌胞外植酸酶对小麦耐盐性的影响[J].应用与环境生物学报,2009,15(01):39-43.[doi:10.3724/SP.J.1145.2009.00039]
 GUO Ying,LIU Dong,ZHAO Lei.Effect of Extracellular Phytase Produced by Bacillus subtilis T2 on Salt Tolerance of Wheat Seedlings[J].Chinese Journal of Applied & Environmental Biology,2009,15(01):39-43.[doi:10.3724/SP.J.1145.2009.00039]
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生防枯草芽孢杆菌胞外植酸酶对小麦耐盐性的影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
15卷
期数:
2009年01期
页码:
39-43
栏目:
研究论文
出版日期:
2009-02-25

文章信息/Info

Title:
Effect of Extracellular Phytase Produced by Bacillus subtilis T2 on Salt Tolerance of Wheat Seedlings
作者:
郭英刘栋赵蕾
山东师范大学生命科学学院 济南 250014
Author(s):
GUO Ying LIU Dong ZHAO Lei
College of Life Sciences, Shandong Normal University, Jinan 250014, China
关键词:
枯草芽孢杆菌胞外植酸酶诱变小麦盐胁迫
Keywords:
Bacillus subtilis extracellular phytase mutation wheat seedlings salt stress
分类号:
S482.8 : Q948.113
DOI:
10.3724/SP.J.1145.2009.00039
文献标志码:
A
摘要:
以产植酸酶的广谱拮抗生防枯草芽孢杆菌(Bacillus subtilis) T2为出发菌株,经硫酸二乙酯(DES)诱变,得到遗
传特性稳定的一株植酸酶负突变株M3. 采用有效磷限量、含NaCl 150 mmol L-1的植物生长水培液对小麦进行盐胁迫,
在植酸存在的条件下,水培液中施加T2发酵液或植酸酶均可促进盐胁迫下小麦幼苗的生长,小麦株高、鲜重、叶片叶
绿素含量和根系活力均得以增高,而植株丙二醛(MDA)含量降低. 失去植酸酶活力的负突变株M3发酵液则不具有提
高小麦株高鲜重和叶绿素含量的作用,对根系活力的提高和对MDA含量的降低程度也均低于T2发酵液处理. 上述结
果表明,生防枯草芽孢杆菌胞外植酸酶在增强小麦耐盐性方面起了重要作用. 图6 表2 参13
Abstract:
Strain Bacillus subtilis T2 is a potential biocontrol agent against a broad-spectrum plant pathogenic fungi and
can produce extracellular phytase. To evaluate whether the phytase secreted by T2 can enhance the saline tolerance of plant, mutagenetic program was undertaken for the construction of extracellular phytase derivates. A phytase minus mutant M3 with genetic stability was obtained by means of DES treatment. The results from biochemical experiments revealed that the culture filtrates of wild-type strain T2 with phytase activity and purified phytase stimulated growth of wheat seedlings under phosphate limitation in the presence of 150 mmol L-1 NaCl and phytate. Significant increases in plant height, fresh weight, total leaf chlorophyll content and TTC reducing power and decrease in content of MDA were observed in response to the addition of phytase and culture filtrates of T2 to culture liquids. However, the culture filtrates obtained from phytase-negative mutant strain M3 did not stimulate the growth of wheat seedlings under salt stress. These may provide strong evidence that phytase activity of B. subtilis is important for the increasing resistance of wheat to salt stress under phosphate limitation. Fig 6, Tab 2, Ref 13

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备注/Memo

备注/Memo:
国家“十一五”科技支撑计划重点项目(No. 2006BAD17B07)和山东省自然
科学基金项目(No. Y2005D06)共同资助
更新日期/Last Update: 2009-03-05