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[1]堵国成,陈坚,高海军,等.真养产碱杆菌ATCC17699生产聚β-羟基丁酸摇瓶发酵条件的研究[J].应用与环境生物学报,1996,2(03):308-314.
 Du Guocheng,Chen Jian,Gao Haijun,et al.FERMENTATION CONDITIONS OF POLY HYDROXYBUTYRIC ACID PRODUCTION WITH ALCALIGENES EUTROPHUS ATCC 17699[J].Chinese Journal of Applied & Environmental Biology,1996,2(03):308-314.
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真养产碱杆菌ATCC17699生产聚β-羟基丁酸摇瓶发酵条件的研究()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
2卷
期数:
1996年03期
页码:
308-314
栏目:
论文
出版日期:
1996-08-25

文章信息/Info

Title:
FERMENTATION CONDITIONS OF POLY HYDROXYBUTYRIC ACID PRODUCTION WITH ALCALIGENES EUTROPHUS ATCC 17699
作者:
堵国成 陈坚 高海军 华兆哲 伦世仪
无锡轻工大学生物工程学院环境生物技术研究室 无锡 214036
Author(s):
Du Guocheng Chen Jian Gao Haijun Hua Zhaozhe Lun Shiyi
Environ Biotechnol Lab, School Bioengin, Wuxi Univ Light Industry, Wuxi 214036
关键词:
真养产碱杆菌聚β-羟基丁酸发酵
Keywords:
Alcaligenes eutrophuspoly hydroxybutyric acidfermentation
摘要:
在摇瓶条件下,首先对A.eutrophus生产PHB的一步法和二步法进行了比较研究,然后探讨了A.eutrophus对不同碳源的利用情况,提出了较佳的PHB发酵条件,并分析了PHB的发酵过程曲线。研究结果表明,本研究采用的A.eutrophus以一步法生产PHB较为适宜,其对3种不同碳源的利用能力为:果糖>丁酸>葡萄糖;培养基中的丁酸浓度和pH值对PHB发酵的生产指标和动力学参数有较大影响,其最佳值为1.8%φ和pH为0.8;最终发酵过程达到细胞于重ρ=7.2g·L-1,胞内PHB含量ρ=5.0g·L-1,ω(PHB)占细胞干重72.2%.
Abstract:
In shake flasks,one-step and two-step fermentation methods were compared for PHB produchon with A.eutrophus and the utilization of carbon sources by A.eutrophus was investigated.Then,relatively optimal PHB fermentation conditions were put forward and time-coures of PHB fermentation was analyzed.The results indicated that one-step fermentation method was more suitable for PHB production with A.eutrophus and its carbon source utilhation ability was: fructose>butyric acid>glucose.The concentration of butyric acid and pH in the medium greatly affected the kinetic parameters and production criteria of PHB formation,and their optimal values were 1.8% (φ) and pH 8.0 respectively.The maximal cell dry weight of ρ=7.2 g·L-1,intracellular ρ(PHB) = 5.0 g·L-1 and ω(PHB) = 72.2% in the cells (based on cell dry weight)could be obtained.

参考文献/References:

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相似文献/References:

[1]堵国成,陈坚,尹洪波,等.真养产碱杆菌生产聚β-羟基丁酸的流加发酵条件研究[J].应用与环境生物学报,1997,3(04):371.
 Du Guocheng,Chen Jian,Yin Hongbo,et al.PRODUCTION OF POLY-β-HYDROXYBUTYRIC ACID BY FED-BATCH CULTURE WITH ALCALIGENESI EUTROPHUS[J].Chinese Journal of Applied & Environmental Biology,1997,3(03):371.

备注/Memo

备注/Memo:
收稿日期:1996-1-15;接受日期:1996-5-17。
更新日期/Last Update: 1900-01-01