|本期目录/Table of Contents|

[1]耿瑞双,缪树华,何立明.抗赖氨酸加苏氨酸的玉米种子蛋白突变体[J].应用与环境生物学报,1995,1(01):1-6.
 Geng Ruishuang,Miao Shuhua,He Liming.MAIZE SEED PROTEIN MUTANTS RESISTANT TO LYSINE PLUS THREONINE[J].Chinese Journal of Applied & Environmental Biology,1995,1(01):1-6.
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抗赖氨酸加苏氨酸的玉米种子蛋白突变体()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
1卷
期数:
1995年01期
页码:
1-6
栏目:
研究论文
出版日期:
1995-02-25

文章信息/Info

Title:
MAIZE SEED PROTEIN MUTANTS RESISTANT TO LYSINE PLUS THREONINE
作者:
耿瑞双 缪树华 何立明
中国科学院成都生物研究所, 成都 610041
Author(s):
Geng Ruishuang Miao Shuhua He Liming
Chengdu Institute of Biology, Academia Sinica, Chengdu 610041
关键词:
玉米胚乳蛋白突变体高赖氨酸高蛋氨酸组织培养
Keywords:
maizeendosperm protein mutanttissue culturehigh methioninehigh lysine
摘要:
通过离体选择抗赖氨酸加苏氨酸玉米突变体,不仅获得了积累游离苏氨酸和赖氨酸等氨基酸的突变类型,也选择到了种子蛋白组份发生改变的高蛋氨酸突变体和高赖氨酸体细胞无性系变异体.高蛋氨酸种子中总蛋氨酸的含量比对照增高22.6%,这是由于醇溶蛋白Zein-2部分及其蛋氨酸含量增高所致.高赖氨酸变异体种子游离必需氨基酸增高显著(2-10倍),总赖氨酸比对照亲本增高28.1%,含量约占种子干重的0.40%,种子蛋白清蛋白、球蛋白和谷蛋白组份含量增高而醇溶蛋白减少.高赖氨酸和高蛋氨酸特性遗传稳定,植株育性正常.
Abstract:
Through in vitro selection of lysine plus threonine resistant mutant,a high methionine mutant and high lysine variant with changed seed protein composition were obtained. Seed protein methionine was 22.6% higher in the mutant than that of the control.This was caused by an increase of Zein-2 fraction and its methionine level. There was also an over production of seed free threonine. In the seed of high lysine variant,all the free amino acids were increased significantly and the total lysine was increased by 28.1%. The traits of high methionine and high lysine were stably inherited. Both the mutant and variant showed normal fertility.

参考文献/References:

[1] Schaeffer G W,Sharpe F T. Lysine in seed protein from S-aminoethyl-L-cysteine resistant anther-derived tissue cultures of rice. In vitro. 1981,17:345-352
[2] Schaeffer G W.Sharpe F T. Increased lysine and seed storage protein in rice plants recovered from calli selected with inhibitory levels of lysine plus threonine and S-(2-amino-ethyl) cysteine. Plant Physiol.1987,84;509-515
[3] Hibbered K A,Green C E. Inheritance and expression of lysine plus threonine resistance selected in maize tissue culture. Proc Natl Acad Sci USA. 1982,79,559-563
[4] Miao S, Duncan D R. Widholm J M. Selection of regenerable maize callus cultures resistant to 5-methyl-DL-tryptophan,S-2-aminoethyl-L-cysteine and high levels of L-lysine plus L-threonine. Plnrt Cell Tr-cc Org Cult. 1988, 14:3-14
[5] Diedrick T J,Frisch D A,Gengenbach B G. Tissue culture isolation of a second mutation for increased Threonine accumulation in maize.Theor Appl Genet. 1990,79:209-215
[6] Lowry O H,Roserbroughu N J,Farr A L et al. Protein measurement with the Folin phenol reagent. J Bwl Chem.1951.193:265-275
[7] Dotson S B,Frisch D A,Somers D A et al. Lysine-insensitive aspartate kinase in two threonine-overpraJucing mutants of maize.planta.1990.182:546-552

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备注/Memo

备注/Memo:
收稿日期:1994-12-15;接受日期:1995-2-25。
基金项目:国家重点科技攻关,中国科学院重大应用研究,国家自然科学基金项目。
更新日期/Last Update: 1900-01-01