|本期目录/Table of Contents|

[1]陈强,陈文新,张小平,等.四川省部分豆科植物根瘤菌的遗传多样性[J].应用与环境生物学报,2008,14(01):83-089.
 CHEN Qiang,et al..Genetic Diversity of Rhizobia Isolated from Some Legumes in Sichuan, China[J].Chinese Journal of Applied & Environmental Biology,2008,14(01):83-089.
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四川省部分豆科植物根瘤菌的遗传多样性()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
14卷
期数:
2008年01期
页码:
83-089
栏目:
综述
出版日期:
2008-01-15

文章信息/Info

Title:
Genetic Diversity of Rhizobia Isolated from Some Legumes in Sichuan, China
作者:
陈强陈文新张小平李登煜Lindstrom K
(1四川农业大学资源环境学院四川雅安625000) (2中国农业大学生物学院/农业部农业微生物资源及其应用重点开放实验室北京100094) (3赫尔辛基大学应用化学与微生物系芬兰赫尔辛基Fin00014)
Author(s):
CHEN Qiang et al.
(1College of Resources and Environmental Science, Sichuan Agricultural University, Yaan 625000, Sichuan, China) (2Key Laboratory of Agromicrobial Resource and Application, Ministry of Agriculture/College of Biological Sciences,China Agricultural University, Beijing 100094, China) (3Department of Applied Chemistry and Microbiology, University of Helsinki, Helsinki Fin00014, Finland)
关键词:
关键词 豆科植物根瘤菌遗传多样性指纹
Keywords:
Keywords legume rhizobia genetic diversity fingerprint
摘要:
摘要 从四川省豆科植物鸡眼草属、合欢属、豇豆属、葛属、金合欢、杭子梢分离获得了103株根瘤菌,采用AFLP、 BOXAIR-PCR研究了这些菌株的遗传多样性.结果表明,分离自四川省豆科植物6个属的根瘤菌存在明显的遗传多样性, AFLP分析中,全部供试菌株的相似性为21%,全部供试菌株可分为26个AFLP遗传群;BOXAIR-PCR聚类分析结果表明, 103个菌株分在70%相似性水平处,供试菌株被分为18个BOX遗传群.除部分菌株外,多数根瘤菌按宿主类型聚群,同一宿主而地理来源不同的根瘤菌分布于不同的遗传群,表明宿主和地理环境对根瘤菌具有深刻影响. 图3 表1 参9
Abstract:
Abstract The genetic diversity of 103 rhizobial strains from six genera of legumes Kummerowia spp., Vigna spp., Pueraria spp., Albizia spp., Campylotropis spp. and Acacia spp. in different regions of Sichuan Province, China, together with 17 reference strains, was analyzed by AFLP and BOXAIRPCR. The results showed that the genetic diversity was exited among these strains. The analysis of AFLP showed that all the strains formed one group at 21% similarity, and were divided into 26 AFLP genotypes. BOXAIRPCR divided the 103 strains into 18 BOX genotypes at the level of 70% similarity level, respectively. Most of the strains in different places were grouped with their host plants, however, the rhizobial strains isolated from the same host plant in different places were separated into different groups, which demonstrated that both host plants and environmental factors largely affected the genetic aspects of rhizobia. Fig 3, Tab 1, Ref 9

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更新日期/Last Update: 2008-03-05