[1]缪元颖,刘成君,杨顺楷,等.粘红酵母(Rhodotorula glutinis CIBAS A 1401)苯丙氨酸解氨酶cDNA核心序列的克隆与分析[J].应用与环境生物学报,2005,11(06):694-698.
　MIAO Yuanying,et al..Core cDNA cloning and structural analysis of phenylalanine ammonia-lyase of Rhodotorula glutinis CIBAS A 1401[J].Chinese Journal of Applied & Environmental Biology,2005,11(06):694-698.
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粘红酵母(Rhodotorula glutinis CIBAS A 1401)苯丙氨酸解氨酶cDNA核心序列的克隆与分析()

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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

Title:: Core cDNA cloning and structural analysis of phenylalanine ammonia-lyase of Rhodotorula glutinis CIBAS A 1401

Author(s):: MIAO Yuanying; et al.; (Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China) (1College of Life Sciences, Sichuan University, Chengdu 610064, China)

Keywords:: Keywords Rhodotorula glutinis CIBAS A 1401; phenylalanine ammonia lyase; gene cloning; cDNA

摘要:: 摘要 L苯丙氨酸因其在工业、医药上的重要作用而日益受到人们的重视.苯丙氨酸解氨酶(PAL)生物转化肉桂酸生产L苯丙氨酸成了该领域研究与开发的热点.本文利用简并寡核苷酸PCR法结合cDNA末端快速扩增PCR法，从粘红酵母中克隆了苯丙氨酸解氨酶cDNA核心序列，为克隆其cDNA全长序列及体外表达其活性蛋白奠定了基础. 图5 参22

Abstract:: Abstract Lphenylalanine is now an important amino acid for food and medicine industries. The biotransformation of transcinnamic acid to Lphenylalanine by using phenylalanine ammonialyase (PAL) has, therefore, become very important in the research and development of this field. In this paper the gene encoding PAL was cloned by degenerating oligo primed polymerase chain reaction (PCR) and random amplification of cDNA end (RACE) PCR procedure, and a core PAL cDNA sequence cloned was analyzed from Rhodotorula glutinis CIBAS A 1401. Fig 5, Ref 22

[1]缪元颖,李化,杨亚力,等.苯丙氨酸解氨酶高活性红酵母（Rhodotorula sp.）CIBAS A-1401菌株的鉴定[J].应用与环境生物学报,2004,10(02):242.
　MIAO Yuanying,et al..Taxonomy of Rhodotorula sp. CIBAS A 1401 strain with high activety of phenylalanine ammonialyase[J].Chinese Journal of Applied & Environmental Biology,2004,10(06):242.

更新日期/Last Update: 2005-12-28