|本期目录/Table of Contents|

[1]王桂荣,徐广,谭维嘉,等.菊酯类杀虫剂抗性棉铃虫para型钠离子通道基因的部分序列测定与突变[J].应用与环境生物学报,2004,10(03):332-336.
 WANG Guirong,et al..Novel mutation in the parahomologous sodium channel gene associated with nerve insensitivity resistance to pyrethroid in Helicoverpa armigera[J].Chinese Journal of Applied & Environmental Biology,2004,10(03):332-336.
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菊酯类杀虫剂抗性棉铃虫para型钠离子通道基因的部分序列测定与突变()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
10卷
期数:
2004年03期
页码:
332-336
栏目:
综述
出版日期:
2004-06-25

文章信息/Info

Title:
Novel mutation in the parahomologous sodium channel gene associated with nerve insensitivity resistance to pyrethroid in Helicoverpa armigera
作者:
王桂荣徐广谭维嘉吴孔明郭予元
(中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室 北京 100094)
Author(s):
WANG Guironget al.
(State Key Laboratory of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing100094, China)
关键词:
关键词 棉铃虫 钠离子通道基因 点突变 杀虫剂抗性
Keywords:
Keywords Helicoverpa armigera para voltagedependent sodium channel gene point mutation insecticide resistance
摘要:
摘要 昆虫神经系统para型钠离子通道是菊酯类杀虫剂的主要靶标,对10多种昆虫的研究表明,钠离子通道基因发生点突变与昆虫对菊酯类杀虫剂的抗性密切相关.通过RTPCR扩增的方法,我们获得了编码溴氰菊酯抗性和敏感品系棉铃虫钠离子通道域IIIV的cDNA片段.核苷酸序列及推导的氨基酸序列分析结果表明,溴氰菊酯抗性品系棉铃虫钠离子通道基因不存在其它昆虫中报道的kdr和superkdr抗性突变,但是我们发现了一个甲硫氨酸(M)到亮氨酸(L)的新突变,该突变位于域II和III之间.由于昆虫钠离子通道高度保守,因此,该突变很可能与棉铃虫对溴氰菊酯的抗性有关.另外,通过PCR扩增的方法,克隆了棉铃虫钠离子通道域IIIV的基因片段,该片段全长7 334 bp,包含有13个外显子和12个内含子,与果蝇和烟蚜夜蛾相比,内含子在基因中的位置基本一致,进一步表明昆虫钠离子通道基因在进化过程中高度保守. 图5 表1 参22
Abstract:
Abstract Para voltagedependent sodium channel in the nervous system of insects is the primary target site for pyrethroids. Point mutations in para sodium channel gene are tightly pertinent to insects` resistance to pyrethroids in more than a half dozen insect species. In this study, the cDNA fragments encoding domains IIIV of sodium channel gene were cloned from resistant and susceptible strains of Helicoverpa armigera by RTPCR. Sequence comparison between susceptible and nerve insensitive strains showed there were no point mutations of kdr or superkdr associated with knockdown resistance to pyrethroid insecticides. However, a Met to Leu novel point mutation was found and located in the intracellular linker connecting domains II and III. The mutation from Met to Leu is most likely responsible for Helicoverpa armigera resistance to deltamethrin, since sodium channel was highly conservative in insect. In addition, genomic DNA fragment of domains IIIV of sodium channel gene was also cloned by PCR amplification and the fragment was 7 334 bp in length, possessing 13 exons and 12 introns. Compared with that of Drosophila melanogaster and Heliothis virescens, these inserted sites of introns were nearly consistent, which further proved the conservatism of para sodium channel during evolution. Fig 5, Tab 1, Ref 22

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更新日期/Last Update: 2004-06-23