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[1]刘丽辉,蒋慧敏,区宇程,等.南方野生稻内生细菌的分离鉴定及促生作用[J].应用与环境生物学报,2020,26(05):1051-1058.[doi: 10.19675/j.cnki.1006-687x.2020.01022]
 LIU Lihui,JIANG Huimin,et al.Identification and growth promotion of endophytic bacteria isolated from Oryza meridionalis[J].Chinese Journal of Applied & Environmental Biology,2020,26(05):1051-1058.[doi: 10.19675/j.cnki.1006-687x.2020.01022]
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南方野生稻内生细菌的分离鉴定及促生作用()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
26卷
期数:
2020年05期
页码:
1051-1058
栏目:
土壤与农业微生物应用专栏
出版日期:
2020-10-25

文章信息/Info

Title:
Identification and growth promotion of endophytic bacteria isolated from Oryza meridionalis
作者:
刘丽辉蒋慧敏区宇程谭志远彭桂香
1华南农业大学农学院 广州 510642 2暨南大学生命科学技术学院 广州 510642 3华南农业大学资源环境学院 广州 510642 4广东省植物分子育种重点实验室 广州 510642 5广东省微生物信号与作物病害防空重点实验室 广州 510642
Author(s):
LIU Lihui1 2 JIANG Huimin3 OU Yucheng3 TAN Zhiyuan1 4 & PENG Guixiang3 5?
1 College of Agriculture, South China Agricultural University, Guangzhou 510642, China 2 College of Life Science and Technology, Jinan University, Guangzhou 510642, China 3 College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China 4 Guangdong Provincial Key Laboratory of Plant Molecular Breeding, Guangzhou 510642, China 5 Guangdong Provincial Key Laboratory of Microbial Signals and Disease Control, Guangzhou 510642, China
关键词:
野生稻植物内生菌固氮促进生长
Keywords:
wild rice endophytic bacteria nitrogen fixation growth-promoting
DOI:
10.19675/j.cnki.1006-687x.2020.01022
摘要:
为丰富微生物肥料菌种储备库以及向菌肥菌种的选育提供依据,从南方野生稻(Oryza meridionalis)中分离纯化内生细菌,通过十二烷基硫酸钠-聚丙烯酰胺凝胶(SDS-PAGE)全细胞蛋白电泳、插入序列指纹图谱(IS-PCR)聚类分析,对分离的各类群代表菌株进行16S rRNA基因系统发育分析和鉴定,并测试代表菌株固氮、溶磷、解钾、分泌生长素和1-氨基环丙烷-1-羟酸(ACC)脱氨酶、产铁载体等促生特性. 共分离到58株内生细菌,聚为11个类群. 其中菌株JH40、JH50促生能力强:JH40、JH50的固氮酶活性最高,分别为360.61 nmol mL-1 h-1和494.14 nmol mL-1 h-1;JH40、JH50产吲哚乙酸能力分别为27.84 mg/L和29.97 mg/L,ACC脱氨酶活性分别为24.76 ?mol mg-1 h-1和22.94 ?mol mg-1 h-1,JH40、JH50还具有溶磷和解钾能力. 本研究表明南方野生稻内生菌具有丰富的遗传和功能多样性,包含多种优质促生菌,可以作为微生物肥料储备菌种,并有望在绿色农业发展中发挥促进作用. (图2 表5 参31)
Abstract:
This study was conducted to enrich the microbial fertilizer reserve bank and provide the basis for the selection of bacterial strains using Oryza meridionalis as a separation material. The endophytic bacteria were clustered by SDS-PAGE whole cell protein electrophoresis and IS-PCR fingerprints. Based on the analysis of 16S rRNA gene sequences, the phylogenetic relationships of representative strains were determined. Experiments testing the activity of nitrogenase, phosphorus and potassium dissolution, IAA secretion, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, and siderophore were conducted. A total of 58 endophytic bacteria were isolated. All bacteria were clustered into 11 groups. Strains JH40 and JH50 had strong growth-promoting ability. The nitrogenase activity of JH40 and JH50 were the highest, 360.61 nmol mL-1 h-1 and 494.14 nmol mL-1 h-1, respectively. IAA activity of strains JH40 and JH50 were 27.84 mg/L and 29.97 mg/L; ACC deaminase activities were 24.76 mol mg-1 h-1 and 22.94 mol mg-1 h-1, respectively. In addition, strains JH40 and JH50 could dissolve phosphorus and potassium. There are genetically diverse and functional endophytic bacteria that can be isolated from Oryza meridionalis, including some high-quality growth-promoting bacteria. The endophytic bacteria have a strong potential to promote rice growth, can be used as a microbial fertilizer reserve for bacteria, and are expected to play a catalytic role in the development of green agriculture.

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更新日期/Last Update: 2020-10-25