|本期目录/Table of Contents|

[1]蒋心茹,姬高升,刘杨,等.市政污泥胞外聚合物（EPS）的形成过程与解聚方法研究进展[J].应用与环境生物学报,2020,26(05):1282-1289.[doi: 10.19675/j.cnki.1006-687x.2019.10014]
　JIANG Xinru,,et al.Overview of extracellular polymeric substance (EPS) generation and disaggregation in municipal sewage sludge[J].Chinese Journal of Applied & Environmental Biology,2020,26(05):1282-1289.[doi: 10.19675/j.cnki.1006-687x.2019.10014]
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市政污泥胞外聚合物（EPS）的形成过程与解聚方法研究进展()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
26卷
期数:
2020年05期
页码:
1282-1289
栏目:
综述
出版日期:
2020-10-25

文章信息/Info

Title:
Overview of extracellular polymeric substance (EPS) generation and disaggregation in municipal sewage sludge
作者:
蒋心茹; 姬高升; 刘杨; 房俊楠; 许力山; 谢炎东; 闫志英
1中国科学院成都生物研究所，中国科学院环境与应用微生物重点实验室 成都 610041 2中国科学院大学 北京 100049 3环境微生物四川省重点实验室 成都 610041 4广西师范大学 桂林 541004
Author(s):
JIANG Xinru1;  2;  3;  JI Gaosheng1;  2;  3;  LIU Yang1;  2;  3;  FANG Junnan1;  2;  3;  XU Lishan1;  3;  XIE Yandong4 & YAN Zhiying1;  3?
1 Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Science, Chengdu 610041, China 2 University of Chinese Academy of Science, Beijing 100049, China 3 Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu 610041, China 4 Guangxi Normal University, Guilin 541004, China
关键词:
城市污泥; 厌氧消化; 细胞外聚合物; 预处理
Keywords:
municipal sludge;  anaerobic digestion;  extracellular polymeric substance (EPS);  pretreatment
DOI:
10.19675/j.cnki.1006-687x.2019.10014
摘要:
市政污泥厌氧发酵能将污泥所含有机质转化为能源物质甲烷，使污泥实现“减量化”、“无害化”、“资源化”，但发酵效率与污泥胞外聚合物（extracellular polymeric substance，EPS）的降解程度密切相关. 综述了EPS的形成机理、解聚方法及其组分检测技术. 主要结论为EPS由多聚糖类、脂质、蛋白质、DNA以及某些小分子类物质相互结合、聚集而成. 根据物质之间结合的紧密程度，EPS可分为4个层次，因此EPS解聚方法通过对其结构破坏程度不同而表现出不同的EPS溶解率. 解聚方法大致可分为物理、化学和生物3种，其中生物法效果显著、反应条件温和、反应过程能耗低且对环境无二次污染，具有巨大的应用前景. EPS组分检测技术通过监测EPS中金属离子、腐殖质、水分等的迁移以及污泥结构变化，评价EPS降解程度，为理解污泥厌氧发酵过程、提高污泥厌氧发酵效率提供新的视角和观点，同时为实现污泥厌氧发酵沼渣农林化利用提供理论支撑. 最后提出EPS作为有机聚合体，生物预处理法优势明显，能在提高EPS溶解率的同时减少厌氧反应抑制物生成，实现污泥生物质资源的高效安全利用. （图4 表2 参67）
Abstract:
Municipal sewage sludge anaerobic digestion can turn sewage sludge organics into methane, which makes it reduction, harmless, and recyclable. This previous studies demonstrated that the reaction efficiency is strongly linked with extracellular polymeric substance (EPS) degradation. The paper introduces the formation mechanism, disaggregation methods, and detection technologies of EPS. The main ideas include the following: EPS consists of polysaccharides, lipids, proteins, DNA, and smaller molecules bound together. According to the strength of the binding substances, EPS can be classified into four layers from the outside to the center. Therefore, the dissolution rate of EPS is determined by the different destroying degrees for the structure. Disaggregation methods can be classified into three types: physical, chemical, and biological. Biological methods have features that are advantageous. These include significant degradation, mild reaction conditions, low energy consumption, and no secondary pollution. Developing detection technologies evaluate the EPS degradation level by monitoring the migration of metal ions, humics, water, and EPS components, and changes in the sludge structure. These technologies offer new perspectives on the process of sewage sludge anaerobic digestion and how to improve the efficiency of anaerobic digestion. At the same time, they provide theoretical support for the agricultural and forestry utilization of biogas residues. Finally, we propose that, as EPS consists of organic polymers, it can be used more effectively after biological pretreatments because of enhanced dissolution and a reduction in the generation of inhibitors for anaerobic digestion. Only in this way can we utilize sewage sludge biomass appropriately and safely.

参考文献/References:

1 Li DH, Ganczarczyk JJ. Structure of activated sludge floes [J]. Biotechnol Bioeng, 1990, 35 (1): 57-65
2 伟关, 肖莆, 周晓铁, 春丁. 污泥中胞外聚合物EPS的研究进展[J]. 化学工程师, 2009, 6: 35-40 [Wei G, Pu X, Zhou XT, Ding C. Research advances on sludge extracellular polymeric substance (EPS) [J]. Chem Eng, 2009, 6: 35-40]
3 袁冬琴, 王毅力. 活性污泥胞外聚合物EPS的分层组分及其理化性质的变化特征研究 [J]. 环境科学, 2012, 33 (10): 3522-3529 [Yuan DQ, Wang YL. Study on the stratification components of extracellular polymeric substance (EPS) in activated sludge and their variation characteristic in physicochemical properties [J]. Environ Sci, 2012, 33 (10): 3522-3529]
4 陈悦佳, 赵庆良, 柳成才. 冷冻温度对冻融污泥有机物变化的影响[J]. 哈尔滨工业大学学报, 2015, (342): 1-8 [Chen YJ, Zhao QL, Liu CC. Effect of freezing temperature on freeze/thaw sludge organic matter transformation [J]. J Harbin Inst Technol, 2015 (342): 1-8]
5 杭世珺, 傅涛, 戴晓虎, 王凯军, 杨向平, 肖琼. 技术路线没有走通,产业没有融通, 政策缺乏贯通污泥出路困境如何破?[J]. 环境经济, 2019 (342): 34-39 [Hang SJ, Fu T, Dai XH, Wang KJ, Yang XP, Xiao Q. The technical route has not been passed, the industry has not been fused, and the policy has not been connected. How can the sludge exit dilemma be broken? [J]. Environ Econ, 2019 (342): 34-39]
6 严迎燕. 浅谈我国城镇污水处理厂污泥处理处置现状[J]. 广东化工, 2016, 43 (11): 204-205 [Yan YY. Analysis the status quo of domestic sludge treatment and disposal in China [J]. Guangdong Chem Ind, 2016, 43 (11): 204-205]
7 Kor-Bicakci G, Eskicioglu C. Recent developments on thermal municipal sludge pretreatment technologies for enhanced anaerobic digestion [J]. Renew Sust Energy Rev, 2019 (110): 423-443
8 Hanum F, Yuan LC, Kamahara H, Aziz H A, Atsuta Y, Yamada T, Daimon H. Treatment of sewage sludge using anaerobic digestion in Malaysia: current state and challenges [J]. Front Energy Res, 2019, 7: 1-7
9 Liu X, Han Z, Jie Y, Ye T, Fang Y, Nan W, Bao Z. Review of enhanced processes for anaerobic digestion treatment of sewage sludge [C]//Iop Conf Ser: Earth Environ Sci, England: IOPscience, 2018: 1-7
10 王静, 卢宗文, 田顺, 陶俊杰. 国内外污泥研究现状及进展[J]. 市政技术, 2006, 24 (3): 140-144 [Wang J, Lu ZW, Tian S, Tao JJ. Existing state and development of sludgy researches in domestic and foreign [J]. Mumicip Eng Technol, 2006, 24 (3): 140-144]
11 Yang G, Zhang G, Wang H. Current state of sludge production, management, treatment and disposal in China [J]. Water Res, 2015, 78: 60-73
12 郝晓地, 蔡正清, 甘一萍. 剩余污泥预处理技术概览[J]. 环境科学学报, 2011, 31 (1): 1-12 [Hao XD, Cai ZQ, Gan YP. Review of pretreatment technology for excedd sludge [J]. Actasci Circum, 2011, 31 (1): 1-12]
13 Lehne G, Muller A, Schwedes J. Mechanical disintegration of sewage sludge [J]. Water Sci Technol, 2001, 43 (1): 19-26
14 Lu F, Wang J, Shao L, He P. Enzyme disintegration with spatial resolution reveals different distributions of sludge extracellular polymer substances [J]. Biotechnol Biofuels, 2016, 9 (29): 2-14
15 Sheng GP, Yu HQ, Li XY. Extracellular polymeric substances (EPS) of microbial aggregates in biological wastewater treatment systems: a review [J]. Biotechnol Adv, 2010, 28 (6): 882-894
16 Christensen BE. The role of extracellular polysaccharides in biofilmschristensen [J]. J Biotechnol, 1989, 10 (3): 181-202
17 Nielsen PH, Jahn A, Palmgren R. Conceptual model for production and composition of exopolymers in biofilms [J]. Water Sci Technol, 1997, 36 (1): 11-19
18 Wingender J, Neu TR, Flemming HC. Microbial Extracellular Polymeric Substances [M]. Berlin: Springer-Verlag, 1999: 52
19 Liu Y, Xu HL, Yang SF, Tay JH. Mechanisms and models for anaerobic granulation in upflow anaerobic sludge blanket reactor [J]. Water Res, 2003, 37 (3): 661-673
20 Ding Z, Bourven I, Guibaud G, Van Hullebusch ED, Panico A, Pirozzi F, Esposito G. Role of extracellular polymeric substances (EPS) production in bioaggregation: application to wastewater treatment [J]. Appl Microbiol Biotechnol, 2015, 99 (23): 9883-9905
21 Sheng GP, Yu HQ. Characterization of extracellular polymeric substances of aerobic and anaerobic sludge using three-dimensional excitation and emission matrix fluorescence spectroscopy [J]. Water Res, 2006, 40 (6): 1233-1239
22 Ramesh A, Lee DJ, Hong SG. Soluble microbial products (SMP) and soluble extracellular polymeric substances (EPS) from wastewater sludge [J]. Appl Microbiol Biotechnol, 2006, 73 (1): 219-225
23 Li XY, Yang SF. Influence of loosely bound extracellular polymeric substances (EPS) on the flocculation, sedimentation and dewaterability of activated sludge [J]. Water Res, 2007, 41 (5): 1022-1030
24 Yu GH, He PJ, Shao LM, He PP. Stratification structure of sludge flocs with implications to dewaterability [J]. Environ Sci Technol, 2008, 42 (21): 7944-7949
25 Baird BH, Thistle D. Uptake of bacterial extracellular polymer by a deposit-feeding holothurian (Isostichopus badionotus) [J]. Marin Biol, 1986, 92 (2): 183-187
26 Flemming HC, Neu TR, Wozniak DJ. The EPS matrix: the “house of biofilm cells” [J]. J Bacteriol, 2007, 189 (22): 7945-7947
27 Yu GH, Tang Z, Xu YC, Shen QR. Multiple fluorescence labeling and two dimensional FTIR-13C NMR heterospectral correlation spectroscopy to characterize extracellular polymeric substances in biofilms produced during composting [J]. Environ Sci Technol, 2011, 45 (21): 9224-9231
28 王治军, 王伟, 夏州, 吴舒旭. 热水解污泥的厌氧消化试验研究[J]. 中国给水排水, 2003, 19 (9): 1-4 [Wang ZJ, Wang W, Xia Z, Wu SX. Experimental study on thermal hydrolysis and anaerobic digestion of sewage sludge [J]. China Water Wastewater, 2003, 19 (9): 1-4]
29 Kor-Bicakci G, Eskicioglu C. Recent developments on thermal municipal sludge pretreatment technologies for enhanced anaerobic digestion [J]. Renewable Sustainable Energy Rev, 2019, 110: 423-443
30 Jorand F, Zartarian F, Thomas F, Block JC, Bottero JY, Villemin G, Urbain V, Manem J. Chemical and structural (2D) linkage between bacteria within activated sludge flocs [J]. Water Res, 1995, 29 (7): 1639-1647
31 Bhaskar PV, Bhosle NB. Bacterial extracellular polymeric substance (EPS): a carrier of heavy metals in the marine food-chain [J]. Environ Int, 2006, 32 (2): 191-198
32 李丹阳, 陈刚, 张光明. 超声波预处理污泥研究进展[J]. 环境污染治理技术与设备, 2003, 4 (8): 70-72 [Li DY, Chen G, Zhang GM. the research progress in ultrasonic pretreatment of sludge [J]. Techniq Equip Environ Pollut Con, 2003, 4 (8): 70-72]
33 Xu X, Cao D, Wang Z, Liu J, Gao J, Sanchuan M, Wang Z. Study on ultrasonic treatment for municipal sludge [J]. Ultrason Sonochem, 2019, 57: 29-37
34 朱玲. 缺氧-厌氧污泥减量过程中EPS与SMP特征变化规律[D]. 西安: 西安理工大学, 2018 [Zhu L. Study on characteristics of SMP characteristics variation and mechanism of freeze-thaw dewatering anaerobic reduction [D]. Xi’an: Xi’an University of Technology, 2018]
35 简东升, 朱敬平, 李笃中, 彭晓峰. 以天然气候进行污泥的冻融处理[J]. 应用基础与工程科学学报, 2000, 8 (4): 370-375 [Jian DS, Zhu JP, Lin DZ, Peng XF. Freeze-thaw treatment of sludge in natural climate [J]. J Basic Sci Eng, 2000, 8 (4): 370-375]
36 张月. 超声预处理对餐厨垃圾与污泥两级厌氧消化的影响[D]. 苏州: 苏州科技大学, 2016 [Zhang Y. Effect of ultrasonic pretreatment on two-phase anaerobic digestion of food waste and sewage sludge [D]. Suzhou: Suzhou University of Science and Technology, 2016]
37 Zhen G, Lu X, Kato H, Zhao Y, Li YY. Overview of pretreatment strategies for enhancing sewage sludge disintegration and subsequent anaerobic digestion: current advances, full-scale application and future perspectives [J]. Renew Sustain Energy Rev, 2017 (69): 559-577
38 Tyagi VK, Lo SL. Application of physico-chemical pretreatment methods to enhance the sludge disintegration and subsequent anaerobic digestion: an up to date review [J]. Rev Environ Sci Biotechnol, 2011, 10 (3): 215-242
39 Zhang D, Chen Y, Zhao Y, Zhu X. New sludge pretreatment method to improve methane production in waste activated sludge digestion [J]. Environ Sci Technol, 2010, 44 (12): 4802-4808
40 张闻多, 余雷, 刘和, 符波, 郑志永, 刘宏波. 工程规模下碱类型对污泥预处理效果及发酵产酸的影响[J]. 环境工程学报, 2018, 12 (5): 1517-1527 [Zhang WD, Yu L, Liu H, Fu B, Zheng ZY, Liu HB. Effect of alkali type on sludge pretreatment effect and fermentation acid production under project scale [J]. Chin J Environ Eng, 2018, 12 (5): 1517-1527]
41 袁光环, 周兴求, 伍健东. 酸-碱预处理促进剩余污泥厌氧消化的研究[J]. 环境科学, 2012, 33 (6): 1918-1922 [Yuan GH, Zhou XQ, Wu JD. Enhancement of anaerobic digestion of excess sludge by acid-alkali pretreatment [J]. Environ Sci, 2012, 33 (6): 1918-1922
42 Song X, Shi Z, Li X, Wang X, Ren Y. Fate of proteins of waste activated sludge during thermal alkali pretreatment in terms of sludge protein recovery [J]. Front Environ Sci Eng, 2019, 13 (2): 1-9
43 Morgan-Sagastume F, Allen DG. Physicochemical properties and stability of activated sludge flocs under temperature upshifts from 30 to 45 degrees C [J]. J Colloid Interface Sci, 2005, 281 (1): 136-145
44 卢怡清, 许颖, 董滨, 戴晓虎. 去除城市生活污泥中有机络合态金属强化其厌氧生物制气[J]. 环境科学, 2018, 39 (1): 284-291 [Lu YQ, Xu Y, Dong B, Dai XH. Enhance of anaerobic methane production by removal of organic-bonding mentals from sewage sludge [J]. Environ Sci, 2018, 39 (1): 284-291]
45 Wawrzynczyk J, Szewczyk E, Norrlow O, Dey ES. Application of enzymes, sodium tripolyphosphate and cation exchange resin for the release of extracellular polymeric substances from sewage sludge. Characterization of the extracted polysaccharides/glycoconjugates by a panel of lectins [J]. J Biotechnol, 2007, 130 (3): 274-281
46 Sesay ML, Ozcengiz G, Dilek Sanin F. Enzymatic extraction of activated sludge extracellular polymers and implications on bioflocculation [J]. Water Res, 2006, 40 (7): 1359-1366
47 白润英, 陈湛, 张伟军, 王东升. 复合酶处理对活性污泥溶解的动力学机制研究[J]. 环境工程学报, 2016, 10 (10): 5840-5846 [Bai RY, Chen K, Zhang WJ, Wang DS. Kinetic mechanisms study of activated sludge solubilization under composite enzyme treatment [J]. Chin J Environ Eng, 2016, 10 (10): 5840-5846]
48 张宇, 李明智, 梅荣武, 许青兰, 王慧荣, 韦彦斐. 应用柠檬酸和酶制剂协同减量处理活性污泥[J]. 环境工程学报, 2017, 11 (3): 1947-1952 [Zhang Y, Li MZ, Mei RW, Xu QL, Wang HR, Wei YF. Reduction of excess activated sludge production by combine citric acid and enzyme treatment [J] Chin J Environ Eng, 2017, 11 (3): 1947-1952]
49 Yang Q, Luo K, Li XM, Wang DB, Zheng W, Zeng GM, Liu JJ. Enhanced efficiency of biological excess sludge hydrolysis under anaerobic digestion by additional enzymes [J]. Bioresour Technol, 2010, 101 (9): 2924-2930
50 辛晓东. 酶溶剩余污泥发酵产酸效能与机制研究[D]. 哈尔滨: 哈尔滨工业大学, 2018 [Xin XD. Volatile fatty acid production performance and mechanism from waste activated sludge frementation enhanced by enzymolysis [D]. Harbin: Harbin Institute of Technology, 2018]
51 Guo L, Lu M, Li Q, Zhang J, Zong Y, She Z. Three-dimensional fluorescence excitation-emission matrix (EEM) spectroscopy with regional integration analysis for assessing waste sludge hydrolysis treated with multi-enzyme and thermophilic bacteria [J]. Bioresour Technol, 2014, 171: 22-28
52 Sato H, Kuribayashi K, Fujii K. Possible practical utility of an enzyme cocktail produced by sludge-degrading microbes for methane and hydrogen production from digested sludge [J]. New Biotechnol, 2016, 33 (1): 1-6
53 Diak J, Ormeci B, Kennedy KJ. Effect of enzymes on anaerobic digestion of primary sludge and septic tank performance [J]. Bioprocess Biosyst Eng, 2012, 35 (9): 1577-1589
54 曹艾清, 王毅力. 生物酶-CTMAB/CPAM联合调理对活性污泥脱水性能的影响[J]. 环境工程学报, 2017, 11 (8): 4467-4475 [Cao AQ, Wang YL. Effect of combined enzymes-CTMAB/CPAM conditioning on activated sludge dewaterability [J]. Chin J Environ Eng, 2017, 11 (8): 4467-4475]
55 Wen C, Paul W, Leenheer JA, Karl B. Fluorescence excitation-emission matrix regional integration to quantify spectra for dissolved organic matter [J]. Envir Sci Technol, 2015, 37 (24): 5701-5710
56 Monique R, Elisabeth GN, Etienne P, Dominique L. A high yield multi-method extraction protocol for protein quantification in activated sludge [J]. Bioresour Technol, 2008, 99 (16): 7464-7471
57 卢明敏. 基于三维荧光光谱技术的剩余污泥厌氧发酵产氢研究[D]. 青岛: 中国海洋大学, 2014 [Lu MM. Investigation of excess sludge anaerobic fermentation hydrogen based on three-dimensional fluorescence spectrocopy [D]. Qidao: Ocean University of China, 2014]
58 Zhang Z, Guo L, Wang Y, Li F, Zhao Y, Gao M, She Z. Degradation and transformation of extracellular polymeric substances (EPS) and dissolved organic matters (DOM) during two-stage anaerobic digestion with waste sludge [J]. Int J Hydrogen Energy, 2017, 42 (15): 9619-9629
59 Gao P, Guo L, Sun J, Wang Y, She Z, Gao M, Zhao Y. Enhancing the hydrolysis of saline waste sludge with thermophilic bacteria pretreatment: new insights through the evolution of extracellular polymeric substances and dissolved organic matters transformation [J]. Sci Total Environ, 2019, 670: 31-40
60 Lattner D, Flemming H-C, Mayer C. 13C-NMR study of the interaction of bacterial alginate with bivalent cations [J]. Intern J Biol Macromol, 2003, 33 (1-3): 81-88
61 Zhang W, Xu Y, Dong B, Dai X. Characterizing the sludge moisture distribution during anaerobic digestion process through various approaches [J]. Sci Total Environ, 2019, 675: 184-191
62 姚炜婷, 孙水裕, 郑莉, 李楚华, 蔡明山, 许燕滨, 童文锦. 超声波-缺氧/好氧消化过程污泥胞外聚合物和溶出物的变化研究[J]. 环境科学, 2011, 32 (6): 1665-1672 [Yao WT, Sun SY, Zheng L, Li CH, Xu YB, Tong WJ. Variation of extracellular polymeric substance and dissolved substances in sewage sludge anaerobic/aerobic digedtion by ultrasonic treatment [J]. Environ Sci, 2011, 32 (6): 1665-1672]
63 Song WJ, Pan X, Zhang D. Lead complexation of soluble and bound extracellular polymeric substances from activated sludge: characterized with fluorescence spectroscopy and ftir spectroscopy [J]. Biotechnol Biotechnol Equip, 2014, 26 (6): 3371-3377
64 Xie S, Wu Y, Wang W, Wang J, Luo Z, Li S. Effects of acid/alkaline pretreatment and gamma-ray irradiation on extracellular polymeric substances from sewage sludge [J]. Radiation Phys Chem, 2014, 97: 349-353
65 陈思思, 杨殿海, 庞维海, 董滨, 戴晓虎. 我国剩余污泥厌氧转化的主要影响因素及影响机制研究进展[J]. 化工进展, 2019: 1-14 [Chen SS, Yang DH, Pang WH, Dong B, Dai XH. Main influence factors and influencing mechanisms of anaerobic transformation of excess sludge in China [J]. Chem Ind Eng Progr, 2019: 1-14]
66 Romero-Güiza MS, Vila J, Mata-Alvarez J, Chimenos JM, Astals S. The role of additives on anaerobic digestion: a review [J]. Renew Sustain Energy Rev, 2016, 58: 1486-1499
67 Damasceno FRC, Freire DMG, Cammarota MC. Impact of the addition of an enzyme pool on an activated sludge system treating dairy wastewater under fat shock loads [J]. J Chem Technol Biotechnol, 2010, 83 (5): 730-738

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　QIAO Ziru,WEI Huawei,MA Jiaying,et al.Review of the generation of volatile organic compounds during the bio-treatment process of food waste[J].Chinese Journal of Applied & Environmental Biology,2020,26(05):210.[doi:10.19675/j.cnki.1006-687x.2019.04018]
[6]马佳莹,汪冰寒,乔子茹,等.碳基材料对餐厨垃圾厌氧消化效率和微生物群落的影响研究进展[J].应用与环境生物学报,2020,26(03):730.[doi:10.19675/j.cnki.1006-687x.2019.07040]
　MA Jiaying,WANG Binghan,QIAO Ziru & XIE Bing.Effects of carbon-based materials on anaerobic digestion efficiency and microbial community of food waste[J].Chinese Journal of Applied & Environmental Biology,2020,26(05):730.[doi:10.19675/j.cnki.1006-687x.2019.07040]
[7]安彤,吴宗林,庞悦,等.导电材料强化挥发性脂肪酸互营氧化产甲烷菌群的种间直接电子传递研究进展[J].应用与环境生物学报,2021,27(03):800.[doi:10.19675/j.cnki.1006-687x.2020.06009]
　AN Tong,WU Zonglin,PANG Yue,et al.Direct interspecies electron transfer strengthened by conductive materials between syntrophic methanogenic communities of volatile fatty acids[J].Chinese Journal of Applied & Environmental Biology,2021,27(05):800.[doi:10.19675/j.cnki.1006-687x.2020.06009]
[8]聂宇,陈娅婷,孙照勇,等.污水/城市污泥中抗生素对厌氧消化的影响研究进展[J].应用与环境生物学报,2020,26(02):479.[doi:10.19675/j.cnki.1006-687x.2019.05001]
　NIE Yu,CHEN Yating,SUN Zhaoyong,et al.Research progress on the effects of antibiotics on anaerobic digestion in sewage and municipal sludge[J].Chinese Journal of Applied & Environmental Biology,2020,26(05):479.[doi:10.19675/j.cnki.1006-687x.2019.05001]

更新日期/Last Update: 2020-10-25