|本期目录/Table of Contents|

[1]朱永静,路保顺,张舒婷,等.龙眼纤维素合成酶基因家族成员鉴定及表达模式[J].应用与环境生物学报,2020,26(05):1235-1243.[doi: 10.19675/j.cnki.1006-687x.2019.10009]
 ZHU Yongjing,LU Baoshun,ZHANG Shuting,et al.Identification and expression patterns of the cellulose synthase gene family in Dimocarpus longan Lour[J].Chinese Journal of Applied & Environmental Biology,2020,26(05):1235-1243.[doi: 10.19675/j.cnki.1006-687x.2019.10009]
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龙眼纤维素合成酶基因家族成员鉴定及表达模式()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
26卷
期数:
2020年05期
页码:
1235-1243
栏目:
研究论文
出版日期:
2020-10-25

文章信息/Info

Title:
Identification and expression patterns of the cellulose synthase gene family in Dimocarpus longan Lour
作者:
朱永静路保顺张舒婷刘蒲东李小斐赖钟雄林玉玲
福建农林大学园艺植物生物工程研究所 福州 350002
Author(s):
ZHU Yongjing LU Baoshun ZHANG Shuting LIU Pudong LI Xiaofei LAI Zhongxiong & LIN Yuling?
Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
关键词:
龙眼纤维素合成酶生物信息学分析表达分析
Keywords:
Dimocarpus longan L. cellulose synthase bioinformatics analysis expression analysis
DOI:
10.19675/j.cnki.1006-687x.2019.10009
摘要:
为探究龙眼纤维素合成酶(cellulose synthase,CESA)家族的生物学功能,采用生物信息学分析方法进行龙眼CESA(DlCESA)家族成员鉴定、蛋白特性和保守基序分析、分子进化树分析、FPKM值分析,并利用qRT-PCR技术分析DlCESA基因的表达模式. 结果表明,DlCESA家族包含9个基因成员,家族成员内含子数量8-13个不等,且蛋白理化性质相近,均包含15个保守的motif;系统进化树分析表明DlCESA分布于6个分支上;顺式作用元件分析表明DlCESA家族启动子序列含有众多植物激素响应、厌氧响应等元件;FPKM值分析表明DlCESA家族在NEC阶段高表达,大部分成员在体胚发生过程中呈现下调表达趋势;除DlCESA7-2外其余DlCESA基因在龙眼不同组织部位都有所表达. qRT-PCR分析表明,4个DlCESA基因在非胚性阶段表达量较高;DlCESA成员可被ABA和MeJA下调表达. 本研究表明DlCESA可能参与龙眼生殖生长和体胚发生进程,并响应逆境胁迫. (图9 表2 参47)
Abstract:
To clarify the biological function of the cellulose synthase (DlCESA) gene family in longan (Dimocarpus longan Lour.), the gene structure, protein characteristics, conserved motifs, cis-acting elements and promoter, phylogenetic tree, and the FPKM values of DlCESA gene family were analyzed by the bioinformatics methods, and qRT-PCR was further used to detect the expression patterns of DlCESA. The results showed that the DlCESA gene family contained 9 members, and their introns ranged from 8 to 13. The physicochemical properties of the different DlCESA proteins were similar, and 15 conserved motifs were found. Phylogenetic analysis showed that the DlCESA were clustered into six groups; cis-acting elements of promoter sequences analysis showed DlCESA contained many plant hormone cis-acting elements and anaerobic elements. The FPKM values showed that DlCESA genes were highly expressed in non-embryogenic cultures (NEC) and down-regulated in the process of early somatic embryogenesis, and DlCESA genes were expressed in a range of tissues except DlCESA7-2. Meanwhile, qRT-PCR analysis showed that four DlCESA genes showed higher expression levels in the NEC stage, and ABA and MeJA down-regulated the expression of DlCESA genes. This study showed that DlCESA genes might be involved in reproductive growth, the somatic embryogenesis of longan, and abiotic stress response.

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更新日期/Last Update: 2020-10-25