|本期目录/Table of Contents|

[1]朱永静,路保顺,张舒婷,等.龙眼纤维素合成酶基因家族成员鉴定及表达模式[J].应用与环境生物学报,2020,26(05):1235-1243.[doi: 10.19675/j.cnki.1006-687x.2019.10009]
 ZHU Yongjing,LU Baoshun,ZHANG Shuting,et al.Identification and expression patterns of the cellulose synthase gene family in Dimocarpus longan Lour[J].Chinese Journal of Applied & Environmental Biology,2020,26(05):1235-1243.[doi: 10.19675/j.cnki.1006-687x.2019.10009]





Identification and expression patterns of the cellulose synthase gene family in Dimocarpus longan Lour
福建农林大学园艺植物生物工程研究所 福州 350002
ZHU Yongjing LU Baoshun ZHANG Shuting LIU Pudong LI Xiaofei LAI Zhongxiong & LIN Yuling?
Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Dimocarpus longan L. cellulose synthase bioinformatics analysis expression analysis
为探究龙眼纤维素合成酶(cellulose synthase,CESA)家族的生物学功能,采用生物信息学分析方法进行龙眼CESA(DlCESA)家族成员鉴定、蛋白特性和保守基序分析、分子进化树分析、FPKM值分析,并利用qRT-PCR技术分析DlCESA基因的表达模式. 结果表明,DlCESA家族包含9个基因成员,家族成员内含子数量8-13个不等,且蛋白理化性质相近,均包含15个保守的motif;系统进化树分析表明DlCESA分布于6个分支上;顺式作用元件分析表明DlCESA家族启动子序列含有众多植物激素响应、厌氧响应等元件;FPKM值分析表明DlCESA家族在NEC阶段高表达,大部分成员在体胚发生过程中呈现下调表达趋势;除DlCESA7-2外其余DlCESA基因在龙眼不同组织部位都有所表达. qRT-PCR分析表明,4个DlCESA基因在非胚性阶段表达量较高;DlCESA成员可被ABA和MeJA下调表达. 本研究表明DlCESA可能参与龙眼生殖生长和体胚发生进程,并响应逆境胁迫. (图9 表2 参47)
To clarify the biological function of the cellulose synthase (DlCESA) gene family in longan (Dimocarpus longan Lour.), the gene structure, protein characteristics, conserved motifs, cis-acting elements and promoter, phylogenetic tree, and the FPKM values of DlCESA gene family were analyzed by the bioinformatics methods, and qRT-PCR was further used to detect the expression patterns of DlCESA. The results showed that the DlCESA gene family contained 9 members, and their introns ranged from 8 to 13. The physicochemical properties of the different DlCESA proteins were similar, and 15 conserved motifs were found. Phylogenetic analysis showed that the DlCESA were clustered into six groups; cis-acting elements of promoter sequences analysis showed DlCESA contained many plant hormone cis-acting elements and anaerobic elements. The FPKM values showed that DlCESA genes were highly expressed in non-embryogenic cultures (NEC) and down-regulated in the process of early somatic embryogenesis, and DlCESA genes were expressed in a range of tissues except DlCESA7-2. Meanwhile, qRT-PCR analysis showed that four DlCESA genes showed higher expression levels in the NEC stage, and ABA and MeJA down-regulated the expression of DlCESA genes. This study showed that DlCESA genes might be involved in reproductive growth, the somatic embryogenesis of longan, and abiotic stress response.


1 Song L, Yu J, Tian P, Hu X, Wang Q, Pan Y. Genome-wide characterization of the cellulose synthase gene superfamily in Solanum lycopersicum [J]. Gene, 2019, 688: 71-83
2 Kimura S, Laosinchai W, Itoh T, Cui XJ, Linder CR, Brown RM Jr. Immunogold labeling of rosette terminal cellulose-synthesizing complexes in the vascular plant Vigna angularis [J]. Plant Cell, 1999, 11 (11): 2075-2086
3 Bashline L, Li SD, Gu Y. The trafficking of the cellulose synthase complex in higher plants [J]. J Bot, 2014, 114: 1059-1067
4 Manoj K, Simon T. Plant cellulose synthesis: CESA proteins crossing kingdoms [J]. Phytochemistry, 2015, 112: 91-99
5 Rayon C, Olek AT, Carpita NC. Towards edesigning cellulose biosynthesis for improved bioenergy feedstocks [J]. Plants Bioenerg, 2014: 183-193
6 Kaur S, Dhugga KS, Gill K, Singh J. Structural and functional motifs in cellulose synthase (CESA) genes of bread wheat (Triticum aestivum L. ) [J]. PLoS ONE, 2016, 11 (1): 1-18
7 Maleki SS, Mohammadi K, Ji K. Characterization of cellulose synthesis in plant cells [J]. Sci World J, 2016: 8641373
8 Taylor NG, Laurie S, Turner SR. Multiple cellulose synthase catalytic subunits are required for cellulose synthesis in Arabidopsis [J]. Plant Cell, 2000, 12 (12): 2529-2539
9 侯金祥. 甘蔗纤维素合成酶A家族三个关键基因的功能分析[D]. 福州: 福建农林大学, 2018 [Hou JX. Function analysis of three key CESAS in sugarcane [D]. Fuzhou: Fujian Agriculture and Forestry University, 2018]
10 Morello L, Pydiura N, Galinousky D, Blume Y, Breviario D. Flax tubulin and CesA superfamilies represent attractive and challenging targets for a variety of genome- and base-editing applications [J]. Funct Integr Genomics, 2019: 1-14
11 Hee JK, Barbara AT, Zhang HB, Lee MK, Doug JH, Li P, David D. Fang. Cloning and characterization of homeologous cellulose synthase catalytic subunit 2 genes from allotetraploid cotton (Gossypium hirsutum L. ) [J]. Gene, 2012, 494 (2): 181-189
12 Ruprecht C, Proost S, Hernandez M, Ortiz C, Lang D, Rensing S, Becker J, Vandepoele K, Mutwil M. Phylogenomic analysis of gene co-expression networks reveals the evolution of functional modules [J]. Plant J, 2017, 90 (3): 447-465
13 Creux, NM, Ranik M, Berger DK, Myburg AA. Comparative analysis of orthologous cellulose synthase promoters from Arabidopsis, Populus and Eucalyptus: evidence of conserved regulatory elements in angiosperms [J] New Phytol, 2008, 179 (3): 722-737
14 Wang LQ, Guo K, Li Y, Tu YY, Hu HZ, Wang BR, Cui XC, Peng LC. Expression profiling and integrative analysis of the CESA/CSL superfamily in rice [J]. BMC Plant Biol, 2010, 10 (1): 282
15 Zhang B, Deng L, Qian Q. A missense mutation in the transmembrane domain of CESA4 affects protein abundance in the plasma membrane and results in abnormal cell wall biosynthesis in rice [J]. Plant Mol Biol, 2009, 71 (4-5): 509-524
16 Wang DF, Yuan SJ, Yin L, Zhao JF, Guo BT, Lan JH, Li XY. A missense mutation in the transmembrane domain of CESA9 affects cell wall biosynthesis and plant growth in rice [J]. Plant Sci, 2012, 196: 117-124
17 徐宗昌, 孔英珍. 普通烟草CESA基因家族成员的鉴定, 亚细胞定位及表达分析[J]. 遗传, 2017, 39 (6): 512-524 [Xu ZC, Kong YZ. Genome-wide identification, subcellular localization and gene expression analysis of the members of CESA gene family in common tobacco (Nicotiana tabacum L. ) [J]. Hereditas, 2017, 39 (6): 512-524]
18 许雷. 大青杨纤维素合酶基因的克隆与遗传转化的研究[D]. 哈尔滨: 东北林业大学, 2011 [Xu L. Genes cloning and genetic transformation of cellulose synthase in Populus ussuriensis kom [D]. Harbin: Northeast Forestry University, 2011]
19 齐文娥, 陈厚彬, 彭朵芬. 中国龙眼产业发展现状、问题与对策建议[J]. 广东农业科学, 2016, 43 (8): 169-174 [Qi WY, Chen HB, Peng DF. Development status, problems and countermeasures of Longan industry in China [J]. Guangdong Agric Sci, 2016, 43 (8): 169-174]
20 Nawaz MA, Lin X, Chan TF, Imtiaz M, Rehman HM, Ali MA, Baloch FS, Atif RM, Yang SH, Chung GH. Characterization of cellulose synthase A (CESA) gene family in Eudicots [J]. Biochem Genet, 2019, 57 (2): 248-272
21 Lin Y, Min J, Lai R. Genome-wide sequencing of longan (Dimocarpus longan Lour.) provides insights into molecular basis of its polyphenol rich characteristics [J]. Gigascience, 2017, 6 (5): 1-14
22 赖钟雄.龙眼生物技术研究[M]. 福州: 福建科学技术出版社, 2003: 18-22 [Lai ZX. Longan Biotechnology Research [M]. Fuzhou: Fujian Sci-Tech Press, 2003]
23 Xie T, Chen CJ, Li CH, Liu JR, Liu CY, He YH. Genome-wide investigation of WRKY gene family in pineapple: evolution and expression profiles during development and stress [J]. BMC Genomics, 2018, 19 (1): 1-18
24 徐小萍, 陈晓慧, 吕科良, 陈旭, 陈裕坤 林玉玲, 赖钟雄. 龙眼漆酶家族成员全基因组结构与功能分析[J]. 应用与环境生物学报, 2018, 24 (4): 1-16 [Xu XP, Chen XH, Lu KL, Chen X, Chen YK, Lin YL, Lai ZX, Chen XH. Genome-wide identification function analysis of the laccase gene family in Dimocarpus Longan Lour. [J]. Chin J Appl Environ Biol, 2018, 24 (4): 1-16]
25 陈晓慧, 白玉, 李汉生, 陈旭, 林玉玲, 赖钟雄. 龙眼DCL家族基因的启动子分析及时空表达[J]. 西北植物学报, 2017, 37 (10): 1926-1933 [Chen XH, Bai Y, Li HS, Chen X, Lin YL, Lai ZX. Promoter analysis and spatio-temporal expression of DCL family genes in longan [J]. Acta Bot sin NW, 2017, 37 (10): 1926-1933]
26 LAI ZX, LIN YL. Analysis of the global transcriptome of longan (Dimocarpus longan Lour. ) embryogenic callus using Illumina paired-end sequencing [J]. BMC Genomics, 2013, 14 (1): 561
27 Lin YL, Lai ZX. Reference gene selection for qRT-PCR analysis during somatic embryogenesis in longan tree [J]. Plant Sci, 2010, 178 (4): 359-365
28 Persson S, Paredez A, Carroll A, Palsdottir H, Doblin M, Poindexter P, Khitrov N, Auer M, Somerville CR. Genetic evidence for three unique components in primary cell-wall cellulose synthase complexes in Arabidopsis [J]. PNAS, 2007, 104 (39): 15566-15571
29 Stork J, Harris D, Griffiths J, Williams B, Beisson F, Li YH, Mendu V, Haughn, G, DeBolt S. Cellulose synthase 9 Serves a nonredundant role in secondary cell wall synthesis in Arabidopsis Epidermal Testa cells [J]. Plant Physiol, 2010, 153 (2): 580-589
30 熊亚丽, 曹福祥, 刘志明, 董旭杰, 李萌. 珙桐种子败育相关基因CesA的克隆及表达分析[J]. 植物生理学报, 2016, 52 (10): 1481-1490 [Xiong YL, Cao FX, Liu ZM, Dong XJ, Li M. Cloning and expression analysis of CesA genes involved in seed abortion in dove tree (Davidia involucrate)[J]. Plant Physiol, 2016, 52 (10): 1481-1490]
31 刘希强, 张涵, 龚攀, 宫文龙, 王赞. 紫花苜蓿不同发育时期次生壁合成调控的转录组分析[J]. 中国农业科学, 2018, 51 (11): 2049-2059 [Liu XQ, Zhang H, Gong XY, Gong WL, Wang Z. Transcriptome analysis of secondary cell wall synthesis regulation at different developmental stages in alfalfa (Medicago sativa L. ) [J]. Sci Agric Sin, 2018, 51 (11): 2049-2059]
32 张晓榕, 谭俊峰, 温曼晴, 苗震龑. 玉米CesA家族的系统鉴定及功能研究[J]. 西北农林科技大学学报, 2019, 47 (2): 45-53 [Zhang XR, Tan JF, Miao ZY. Systematic identification and functional study of CesA family in maize [J]. J NW A&F Univ, 2019, 47 (2): 45-53]
33 赖钟雄, 陈春玲. 龙眼体细胞胚胎发生过程中的内源激素变化[J]. 热带作物学报, 2002, 23 (2): 41-47 [Lai ZX, Chen CL. Changes of endogenous phytohormones in the process of somatic embryogenesis in longan (Dimocarpus longan Lour. ) [J]. Chin J trop crops, 2002, 23 (2): 41-47]
34 崔凯荣, 邢更生, 周功克, 刘新民, 王亚馥. 植物激素对体细胞胚胎发生的诱导与调节[J]. 遗传, 2000, 22 (5): 349-367 [Cui KS, Xing GS, Zhou GK, Liu XM, Wang FY. The induced and regulatory effects of plant hormones in somatic embryogenesis [J]. Hereditas, 2000, 22 (5): 349-367]
35 魏凯莉, 周厚君, 江成, 赵岩秋, 宋学勤, 卢孟柱. 杨树次生壁纤维素合酶的表达与互作模式分析[J]. 林业科学研究, 2017, 30 (2): 245-253 [Wei KL, Zhou HJ, Jiang C, Zhao YQ, Song XQ, Lu MZ. Interaction and expression of secondary wall CESAs in Populus [J]. For Res, 2017, 30 (2): 245-253]
36 Huang DB, Wang SG, Zhang BC, Guan KS, Shi YY, Zhang DM, Liu XL, Wu K, Xu ZP, Fu XD, Zhou YH. A gibberellin-mediated della-nac signaling cascade regulates cellulose synthesis in rice [J]. Plant Cell, 2015, 27 (6): 1681-1696
37 Hossain Z, McGarvey B, Amyot L, Gruber M, Jung J, Hannoufa A. Diminuto 1 affects the lignin profile and secondary cell wall formation in Arabidopsis [J]. Planta, 2012, 235 (3): 485-498
38 Xie L, Yang C, Wang X. Brassinosteroids can regulate cellulose biosynthesis by controlling the expression of CESA genes in Arabidopsis [J]. J exp bot, 2011, 62 (13): 4495-4506
39 殷雨晴, 任健, 陈典, 王勇. 马占相思AmCesA2基因的克隆与表达分析[J]. 森林与环境学报, 2019, 39 (2): 174-180 [Yin XQ, Ren J, Chen D, Wang y. Cloning and expression analysis of AmCesA2 gene in Acacia mangium [J]. J For Environ, 2019, 39 (2): 174-180]
40 高艳, 陈光辉, 陈秀娟, 谢丽琼. 植物细胞壁纤维素生物合成的调控[J]. 生物技术通报, 2014 (1): 1-7 [Gao Y, Chen GH, Chen XJ, Xie LQ. Regulation of cellulose biosynthesis in plant cell wall [J]. Biotechnol Bull, 2014 (1): 1-7]
41 齐菲. 兴安落叶松纤维素合酶基因的克隆及特性分析[D]. 内蒙古: 内蒙古大学, 2017 [Qi F. Isolation and characterization of cellulose synthase gene from Larix gmelinii [D]. Inner Mongolia: Inner Mongolia University, 2017]
42 姜勇, 胡尚连, 曹颖, 卢学琴, 黄艳, 徐刚. 外源GA3对毛竹实生苗茎秆生长及CesA基因表达的影响[J]. 植物研究, 2017, 37 (5): 744-750 [Jing Y, Hu SL, Cao Y, Lu XQ, Huang Y, Xu G. Effects of exogenous GA3 on the growth of stem and CesA gene expression in Phyllostachys edulis seedling [J]. Bull Bot Res, 2017, 37 (5): 744-750]
43 Thomashow MF. Plant cold acclimation: freezing tolerance genes and regulatory mechanisms [J]. Annu Rev Plant Physiol Plant Mol Biol, 1999, 50: 571-599
44 Kano M, Inukai Y, Kitano H. Root plasticity as the key root trait for adaptation to various intensities of drought stress in rice [J]. Plant Soil, 2011, 342 (1-2): 117-128
45 Chen Z, Hang X, Zhang H.Disruption of the cellulose synthase gene, AtCzsA8/IRXl, enhances drought and osmotic stress tolerance in Arabidopsis [J]. Hant J, 2005, 43 (2): 273-283
46 朱德财, 胡晓晴, 赵桐, 田晶, 张勇, 刘雪梅. 白桦CESA7基因启动子的克隆与表达分析[J]. 植物研究, 2018, 38 (4): 551-558 [Zhu DC, Hu XQ, Zhao T, Tian J, Zhang Y, Liu XM. Cloning and expression analysis of promoter of CESA7 gene in Betula platyphylla [J]. Bull Bot Res, 2018, 38 (4): 551-558]
47 马超, 张均, 王学平, 贾琦石, 原佳乐, 张苏, 冯雅岚. 外源MeJA对花后干旱胁迫下小麦光合特性的影响[J]. 麦类作物学报, 2018, 38 (5): 563-571 [Ma C, Zhang J, Wang XP, Jia Q, Yuan JL, Zhang S, Meng YL. Effect of exogenous methyl jasmonate on photosynthetic characteristics in wheat under drought stress after anthesis [J]. J Tritice Crops, 2018, 38 (5): 563-571


 QIU Dongliang,et al..Injury effects of simulated acid rain on chloroplasts of longan leaves[J].Chinese Journal of Applied & Environmental Biology,2002,8(05):154.
 QIU Dongliang,et al..Effects of simulated acid rain on cellulase activity and contents of endogenous hormone in young fruit of longan[J].Chinese Journal of Applied & Environmental Biology,2004,10(05):35.
 LIN Yuling,LAI Zhongxiong.Cloning of Copper Chaperone for Superoxide Dismutase Gene CCS from Embryogenic Callus of Dimocarpus longan Lour. and Its Expression Analysis During Somatic Embryogenesis[J].Chinese Journal of Applied & Environmental Biology,2012,18(05):351.[doi:10.3724/SP.J.1145.2012.00351]
 LAI Ruilian,LIN Yuling & LAI Zhongxiong**.Cloning of auxin receptor gene TIR1 and its interaction with miR393 in Dimocarpus longan Lour.[J].Chinese Journal of Applied & Environmental Biology,2016,22(05):95.[doi:10.3724/SP.J.1145.2015.05051]
 CHEN Xu,ZENG Youjing,WANG Jiayi,et al.Effect of main grain components on the starch swelling power of Tibetan hull-less barley (Hordeum vulgare var. nudum)[J].Chinese Journal of Applied & Environmental Biology,2017,23(05):602.[doi:10.3724/SP.J.1145.2017.03011]
 WANG Yun,PENG Liyun,SUN Xueli,et al.Genome-wide identification of longan Hsf family members and their functional analysis during somatic embryogenesis in longan[J].Chinese Journal of Applied & Environmental Biology,2019,25(05):420.[doi:10.19675/j.cnki.1006-687x.2018.06004]
 SU Liyao,HUANG Shuqi,JIANG Mengqi,et al.The response patterns of miR403 and its candidate targets to exogenous hormones and their expression profiles in the longan somatic embryo[J].Chinese Journal of Applied & Environmental Biology,2019,25(05):977.[doi:10.19675/j.cnki.1006-687x.2019.03058]
 LI Hansheng,SUN Gang,CHEN Xiaohui,et al.Genome-wide identification and response light expression analysis of the BRI1 family in Dimocarpus longan Lour[J].Chinese Journal of Applied & Environmental Biology,2020,26(05):125.[doi:10.19675/j.cnki.1006-687x.2019.07053]
 LIU Pudong,ZHANG Shuting,CHEN Xiaohui,et al.Genomic identification and expression patterns of the longan GRF family[J].Chinese Journal of Applied & Environmental Biology,2020,26(05):236.[doi:10.19675/j.cnki.1006-687x.2019.06028]
 LIAO Bin,XU Xiaoping,LI Shanshan,et al.Effects of phenylalanine and methyl jasmonate on the growth and corilagin accumulation of embryogenic suspension cells in Dimocarpus longan Lour.[J].Chinese Journal of Applied & Environmental Biology,2020,26(05):287.[doi:10.19675/j.cnki.1006-687x.2019.06001]

更新日期/Last Update: 2020-10-25