|本期目录/Table of Contents|

[1]牟凌,吴宏蕾,顾国军,等.云南岩溶断陷盆地4种植被类型土壤微生物和酶活性特征[J].应用与环境生物学报,2020,26(05):1081-1086.[doi:10.19675/j.cnki.1006-687x.2019.09065]
 MOU Ling,WU Honglei,GU Guojun,et al.Soil microbes and enzyme activities in four vegetation types in Yunnan karst faulted basin[J].Chinese Journal of Applied & Environmental Biology,2020,26(05):1081-1086.[doi:10.19675/j.cnki.1006-687x.2019.09065]
点击复制

云南岩溶断陷盆地4种植被类型土壤微生物和酶活性特征()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
26卷
期数:
2020年05期
页码:
1081-1086
栏目:
土壤与农业微生物应用专栏
出版日期:
2020-10-25

文章信息/Info

Title:
Soil microbes and enzyme activities in four vegetation types in Yunnan karst faulted basin
作者:
牟凌吴宏蕾顾国军林玉徐振锋
1四川农业大学生态林业研究所,四川省林业生态工程重点实验室 成都 6111302成都市温江区东辰外国语学校 成都 6111303四川省阿坝藏族羌族自治州川西林业局 理县 623102
Author(s):
MOU Ling1 WU Honglei2 GU Guojun3 LIN Yu1 & Xu Zhenfeng1?
1 Key Laboratory of Ecological Forestry Engineering of Sichuan Province, Institute of Ecology & Forest, Sichuan Agricultural University, Chengdu 611130, China2 Chengdu Wenjiang Dongchen International School, Chengdu 611300, China3 Forestry Bureau of Western Sichuan, Aba Tibetan and Qiang Autonomous Prefecture of Sichuan, Lixian 623102, China
关键词:
微生物含量酶活性断陷盆地植物群落
Keywords:
microbial content enzyme activity fault basin vegetation type
DOI:
10.19675/j.cnki.1006-687x.2019.09065
摘要:
土壤微生物群落组成及酶活性是反映土壤肥力的重要指标,且与地貌特征和植被群落密切相关. 为了解岩溶断陷盆地典型植被下土壤生物特征,以云南建水县典型岩溶断陷盆地的4种植被类型——玉米、柑橘、石榴和马尾松为研究对象,采用磷脂脂肪酸生物标记法和酶标板法,对比分析不同植被类型土壤微生物群落和酶活性差异. 结果显示,相比3种人工种植植被,马尾松人工林土壤的有机质、全氮和全磷含量均相对较高. 4种植被土壤磷脂脂肪酸总量和细菌生物量总体呈现马尾松林≈柑橘林>玉米地>石榴林,而真菌生物量表现为石榴林显著高于其他3种植被类型. 各类土壤酶在马尾松林下表现出较高的活性,β-葡萄糖苷酶、多酚氧化酶、纤维二糖水解酶和N-乙酰-β-D-葡萄糖苷酶均显著高于其他3种植被类型. 相关分析表明多数土壤酶与土壤有机碳、全氮和全磷含量呈现显著正相关. 综上所述,就土壤生物特征而言,人工种植植被(玉米、柑橘和石榴)在一定程度上降低了土壤的生物活性,而柑橘属于该区域较好的种植模式. (图2 表3 参29)
Abstract:
Soil microbial composition and activity is recognized as an important indicator of soil fertility and is closely related to landform type and vegetation type. In order to explore the soil biological characteristics of different vegetation types in the karst faulted basin, four models (maize, orange, pomegranate, and pine) were chosen as the study area. Soil phospholipid fatty acids (PLFAs) content and enzymatic activities of four planting patterns (maize, orange, pomegranate, and pine models) in the typical faulted basin of Jianshui County of Yunnan Province were compared and analyzed. The total PLFAs and bacterial biomass showed an order of pine ≈ orange > maize > pomegranate, while the fungal biomass was higher in pomegranate pattern than in the other three types. All soil enzymes exhibited higher activities in pine forest than in the other three types. Fungal biomass was significantly negatively correlated with organic matter content. Most soil enzymes were significantly or significantly positively correlated with organic carbon, total nitrogen, and total phosphorus. The artificial planting model (maize, orange, and pomegranate) tended to reduce soil nutrients and biological biomass and activity, and citrus is a relatively good planting model in the region.

参考文献/References:

1 He RY, Yang KJ, Li ZJ, Sch?dler M, Yang WQ, Wu FZ, Tan B, Zhang L, Xu ZF. Effects of forest conversion on soil microbial communities depend on soil layer on the eastern Tibetan Plateau of China [J]. PLoS ONE, 2017, 12 (10): e186053
2 Cavaglieri L, Orlando J, Etcheverry M. Rhizosphere microbial community structure at different maize plant growth stages and root locations [J]. Microbiol Res, 2009, 164 (4): 391-399
3 Ushio M, Balser TC, Kitayama K. Effects of condensed tannins in conifer leaves on the composition and activity of the soil microbial community in a tropical montane forest [J]. Plant Soil, 2013, 365 (1-2): 157-170
4 Li H, Ye DD, Wang XG, Settles ML, Wang Jun, Hao ZQ, Zhou LS, Dong P, Jiang Y, Ma ZS. Soil bacterial communities of different natural forest types in Northeast China [J]. Plant Soil, 2014, 383 (1-2): 203-216
5 Romaniuk R, Giuffré L, Costantini A, Bartoloni N and Nannipieri P. A comparison of indexing methods to uate quality of soils: the role of soil microbiological properties [J]. Soil Res, 2011, 49 (8): 733
6 刘顺, 吴珍花, 郭晓敏, 盛可银, 朱新传, 温卫华, 张文元. 不同林龄陈山红心杉土壤微生物群落结构特征[J]. 应用与环境生物学报, 2016, 22 (3): 510-517 [Liu S, Wu ZH, Guo XM, Sheng KY, Zhu XZ, Wen WH, Zhang WY. Soil microbial community structure characteristics of Chenshan Red-heart Chinese fir of different stand ages [J]. Chin J Appl Environ Biol, 2016, 22 (3): 510-517]
7 于德良, 雷泽勇, 赵国军, 周晏平, 于东伟, 卢慧, 孔涛. 土壤酶活性对沙地樟子松人工林衰退的响应[J]. 环境化学, 2019, 38 (1): 97-105 [Yu DL, Lei ZY, Zhao GJ, Zhou YP, Yu DW, Lu H, Kong T. Response of soil enzyme activity to the decline of Pinus sylvestris var. mongolica plantations on sand land [J]. Environ Chem, 2019, 38 (1): 97-105]
8 曹慧, 孙辉, 杨浩, 孙波, 赵其国. 土壤酶活性及其对土壤质量的指示研究进展[J]. 应用与环境生物学报, 2003, 9 (1): 105-109 [Cao H, Sun H, Yang H, Sun B, Zhao QG. A review of soil enzyme activity and its indication for soil quality [J]. Chin J Appl Environ Biol, 2003, 9 (1): 105-109]
9 孔涛, 刘民, 淑敏, 王凯, 吕刚. 低分子量有机酸对土壤微生物数量和酶活性的影响[J]. 环境化学, 2016, 35 (2): 348-354 [Kong T, Liu M, Shu M, Wang K, Lü G. Effect of low molecular weight organic acids on soil microbe number and soil enzyme activities [J]. Environ Chem, 2016, 35 (2): 348-354]
10 孙鹏跃, 徐福利, 王渭玲, 王玲玲, 牛瑞龙, 高星, 白小芳. 华北落叶松人工林地土壤养分与土壤酶的季节变化及关系[J]. 浙江农林大学学报, 2016, 33 (6): 944-952 [Sun YP, Xu FL, Wang WL, Wang LL, Niu RL, Gao X, Bai XF. Seasonal dynamics of soil nutrients and soil enzyme activities in Larix principis-rupprechtii plantations [J]. J Zhejiang A&F Univ, 2016, 33 (6): 944-952]
11 杨慧, 朱同彬, 王修华, 蒲俊兵, 李建鸿, 张陶, 曹建华. 云南断陷盆地高原面典型小流域土壤元素含量特征[J]. 生态环境学报, 2018, 27 (5): 859-865 [Yang H, Zhu TB, Wang XH, Pu JB, Li JH, Zhang T, Cao JH. Soil element contents of typical small watershed in the plateau area of Karst fault basin, Yunnan [J]. Ecol Environ, 2018, 27 (5): 859-865]
12 刘方, 王世杰, 刘元生, 何腾兵, 罗海波, 龙健. 喀斯特石漠化过程土壤质量变化及生态环境影响评价[J]. 生态学报, 2005, 25 (3): 639-644 [Liu F, Wang SJ, Liu YS, He TB, Luo HB, Long J. Changes of soil quality in the process of Karst rocky desertification and uation of impact on ecological environment [J]. Acta Ecol Sin, 2005, 25 (3): 639-644]
13 盛茂银, 刘洋, 熊康宁. 中国南方喀斯特石漠化演替过程中土壤理化性质的响应[J]. 生态学报, 2013, 33 (19): 6303-6313 [Sheng MY, Liu Y, Xiong KN. Response of soil physical-chemical properties to rocky desertification succession in South China Karst [J]. Acta Ecol Sin, 2013, 33 (19): 6303-6313]
14 颜萍, 熊康宁, 王恒松, 杨皓, 胡晚枚. 喀斯特地区不同等级石漠化对土壤性质的响应[J]. 南方农业学报, 2016, 47 (4): 557-563 [Yan P, Xiong KN, Wang HS, Yang H, Hu WM. Response of soil properties to different grades of rocky desertification in Karst area [J]. J S Agric, 2016, 47 (4): 557-563]
15 邹志刚, 张浩, 曾昭霞, 张建云, 赵杰军, 包松莲, 曾馥平. 云南断陷盆地喀斯特4种典型种植模式的能值分析[J]. 应用生态学报, 2018, 29 (8): 2641-2650 [Zou ZG, Zhang H, Zeng ZX, Zhang JY, Zhao JJ, Bao SL, Zeng FP. Emergy analysis of four typical planting modes in Karst faulted basins of Yunnan Province, China [J]. Chin J Appl Ecol, 2018, 29 (8): 2641-2650]
16 Bossio DA, Scow KM. Impacts of carbon and flooding on soil microbial communities: phospholipid fatty acid profiles and substrate utilization patterns [J]. Microb Ecol, 1998, 35 (3): 265-278
17 Sinsabaugh RL, Reynolds H, Long TM. Rapid assay for amidohydrolase (urease) activity in environmental samples [J]. Soil Biol Biochem, 2000, 32 (14): 2095-2097.
18 楚海燕, 李若南, 李靖雯, 钟兆全, 刘小飞, 李一清. 中亚热带森林转换对土壤微生物群落结构的影响[J]. 应用与环境生物学报, 2019, 25 (1): 23-28 [Chu HY, Li RN, Li JW, Zhong ZQ, Liu XF, Li YQ. Effects of forest conversion on soil microbial community structure [J]. Chin J Appl Environ Biol, 2019, 25 (1): 23-28]
19 罗蓉, 杨苗, 余旋, 马梓桐, 郝汝倩, 杨琳. 沙棘人工林土壤微生物群落结构及酶活性的季节变化[J]. 应用生态学报, 2018, 29 (4): 1163-1169 [Luo R, Yang M, Yu X, Ma ZT, Hao RQ, Yang L. Seasonal dynamics of soil microbial community and enzyme activities in Hippophae rhamnoides plantation [J]. Chin J Appl Ecol, 2018, 29 (4): 1163-1169]
20 彭琳, 王晓君, 黄从德, 李开志. 凋落物输入改变对慈竹林土壤有机碳的影响[J]. 水土保持通报, 2014, 34 (1): 129-132 [Peng L, Wang XJ, Huang CD, Li KZ. Effects of litter input change on soil organic carbon in Dendrocalamus affinnis forest [J]. Bull Soil Water Conserv, 2014, 34 (1): 129-132]
21 王峻, 薛永, 潘剑君, 郑宪清, 秦秦, 孙丽娟, 宋科. 耕作和秸秆还田对土壤团聚体有机碳及其作物产量的影响[J]. 水土保持学报, 2018, 32 (5): 121-127 [Wang J, Xue Y, Pan JJ, Zheng XQ, Qin Q, Sun LJ, Song K. Effects of tillage and straw incorporation on sequestration of organic carbon and crop yields [J]. J Soil Water Conserv, 2018, 32 (5): 121-127]
22 Fioretto A, Papa S, Pellegrino A, Fuggi A. Decomposition dynamics of Myrtus communis and Quercus ilex leaf litter mass loss, microbial activity and quality change [J]. Appl Soil Ecol, 2007, 36 (1): 32-40
23 关松荫, 沈桂琴, 孟昭鹏, 姚造华, 闵九康. 我国主要土壤剖面酶活性状况[J]. 土壤学报, 1984, 21 (4): 368-381 [Guan SM, Shen GQ, Meng ZP, Yao ZH, Min JK. Enzyme activity status of main soil profiles in China [J]. Acta Pedol Sin, 1984, 21 (4): 368-381]
24 陈恩凤. 关于土壤肥力实质研究的来源与设想[J]. 土壤肥料, 1978 (6): 1-5 [Chen EF. Sources and assumptions about the study of soil fertility [J]. China Soils Fert, 1978 (6): 1-5]
25 袁亚玲, 张丹桔, 张艳, 李勋, 陈亚梅, 覃宇, 张健. 马尾松与阔叶树种凋落叶混合分解初期的酶活性[J]. 应用与环境生物学报, 2018, 24 (3): 508-517 [Yuan YL, Zhang DJ, Zhang Y, Li X, Chen YM, Qin Y, Zhang J. Enzyme activities in the early stage of mixed leaf litter decomposition from Pinus massoniana and broad-leaved tree species [J]. Chin J Appl Environ Biol, 2018, 24 (3): 508-517 ]
26 褚洪龙, 李莎, 唐明. 黄土高原油松根际土壤酶活性及真菌群落多样性研究——以黄龙山林场为例[J]. 土壤学报, 2015, 52 (1): 154-161 [Chu HL, Li S, Tang M. Soil enzyme activity and fungal community diversity in rhizosphere of Pinus tabulaeformis Carr. growing on Loess Plateau—a case study of Huanglongshan Forest Farm [J]. Acta Pedol Sin, 2015, 52 (1): 154-161]
27 刘亚军, 吴娟, 邹锋, 韩丽丽, 吴兰. 鄱阳湖湿地灰化薹草洲滩土壤微生物和酶特性对水分梯度的响应[J]. 湿地科学, 2017, 15 (2): 269-275 [Liu YJ, Wu J, Zou F, Han LL, Wu L. Response of properties of soil microbes and enzymes in beach covered by carex cinearascens in Poyang Lake wetlands to moisture gradient [J]. Wetlands, 2017, 15 (2): 269-275]
28 Fujii K, Uemura M, Hayakawa C, Funakawa S, Kosaki T. Environmental control of lignin peroxidase, manganese peroxidase, and laccase activities in forest floor layers in humid Asia [J]. Soil Biol Biochem, 2013, 57: 109-115
29 魏圣钊, 李林, 骆晓, 谭靖星, 刘雄, 刘行, 杨帅, 曹芹, 黄从德. 不同连栽代次的巨桉人工林土壤酶活性及其与土壤理化性质的关系[J]. 应用与环境生物学报, 2019, 25 (6): 1312-1318 [Wei SD, Li L, Luo X, Tan JX, Liu X, Liu X, Yang S, Cao Q, Huang CD. Soil enzyme activities and their relationships to soil physicochemical properties indifferent successive rotation plantation of Eucalyptus grandis [J]. Chin J Appl Environ Biol, 2019, 25 (6): 1312-1318

相似文献/References:

[1]史艳霞,张永军,王桂荣,等.Bt抗性和敏感棉铃虫幼虫中肠主要蛋白酶活性的变化[J].应用与环境生物学报,2008,14(03):394.
 SHI Yanxia,et al..Changes in Mid-gut Protease Activities in Larvae of Bt Resistant and Susceptible Strains of Helicoverpa armigera Hübner[J].Chinese Journal of Applied & Environmental Biology,2008,14(05):394.
[2]李茵** 罗翠 沈国.蛋白质和磷酸水解酶在废水处理系统中的活性和作用[J].应用与环境生物学报,2008,14(04):514.
[3]魏艳红,熊鹰,袁永泽,等.纤维素酶产生菌HS-F9的筛选鉴定和产酶条件优化[J].应用与环境生物学报,2010,16(02):274.[doi:10.3724/SP.J.1145.2010.00274]
 WEI Yanhong,XIONG Ying,YUAN Yongze,et al.Screening and Identification of a Cellulase-producing Fungus and Optimization of Its Fermentation Conditions[J].Chinese Journal of Applied & Environmental Biology,2010,16(05):274.[doi:10.3724/SP.J.1145.2010.00274]
[4]吴尧,万霞,张银波,等.白地霉(lip42)脂肪酶的纯化及酶学性质[J].应用与环境生物学报,2010,16(05):710.[doi:10.3724/SP.J.1145.2010.00710]
 WU Yao,WAN Xia,ZHANG Yingbo,et al.Purification and Properties of a Lipase from Geotrichum candidum lip42[J].Chinese Journal of Applied & Environmental Biology,2010,16(05):710.[doi:10.3724/SP.J.1145.2010.00710]
[5]张宇,乌恩,李重祥,等.长江中下游湖泊沉积物酶活性及其与富营养化的关系[J].应用与环境生物学报,2011,17(02):196.[doi:10.3724/SP.J.1145.2011.00196]
 ZHANG Yu,WU En,LI Chongxiang,et al.Enzyme Activity in Sediments and Its Relation with Eutrophication in the Lakes along the Yangtze River[J].Chinese Journal of Applied & Environmental Biology,2011,17(05):196.[doi:10.3724/SP.J.1145.2011.00196]
[6]田启建,林永慧,何兴兵,等.耐高盐撕裂蜡孔菌P2对模拟橙黄G染料废水的脱色及废水脱色前后的毒性测试[J].应用与环境生物学报,2011,17(06):876.[doi:10.3724/SP.J.1145.2011.00876]
 TIAN Qijian,LIN Yonghui,HE Xingbing,et al.Toxicity Test and Decolorization of Simulated Orange G Dye Wastewater by Ceriporia lacerata P2 with a High-salinity Tolerance[J].Chinese Journal of Applied & Environmental Biology,2011,17(05):876.[doi:10.3724/SP.J.1145.2011.00876]
[7]胡蓉,张姝,吴建伟,等.家蝇纤维素酶的检测及酶活性与其发育的关系[J].应用与环境生物学报,2013,19(03):444.[doi:10.3724/SP.J.1145.2013.00444]
 HU Rong,ZHANG Shu,WU Jianwei,et al.Detection of Cellulases and the Relationship Between Enzyme Activity and Development of Musca domestica[J].Chinese Journal of Applied & Environmental Biology,2013,19(05):444.[doi:10.3724/SP.J.1145.2013.00444]
[8]王文,杨波.硫脲对大豆苗期的毒理效应[J].应用与环境生物学报,2015,21(01):175.[doi:10.3724/SP.J.1145.2014.05023]
 WANG Wen,YANG Bo.Phytotoxicity of thiourea application on soybean seedlings[J].Chinese Journal of Applied & Environmental Biology,2015,21(05):175.[doi:10.3724/SP.J.1145.2014.05023]
[9]李明锐,钟伟,杨志新,等.矿区周边农田冬种蚕豆、油菜对土壤性质的影响及作物镉铅累积特征[J].应用与环境生物学报,2017,23(05):845.[doi:10.3724/SP.J.1145.2016.11015]
 LI Mingrui,ZHONG Wei,YANG Zhixin,et al.Effects of cultivation of faba bean and rape in winter on partial properties of soil in farmlands surrounding mining area and characteristics of cadmium and lead accumulation in crops[J].Chinese Journal of Applied & Environmental Biology,2017,23(05):845.[doi:10.3724/SP.J.1145.2016.11015]
[10]袁亚玲,张丹桔,张艳,等.马尾松与阔叶树种凋落叶混合分解初期的酶活性[J].应用与环境生物学报,2018,24(03):508.[doi:10.19675/j.cnki.1006-687x.2017.08042]
 YUAN Yaling,ZHANG Danju,ZHANG Yan,et al.Enzyme activities in the early stage of mixed leaf litter decomposition from Pinus massoniana and broad-leaved tree species[J].Chinese Journal of Applied & Environmental Biology,2018,24(05):508.[doi:10.19675/j.cnki.1006-687x.2017.08042]

更新日期/Last Update: 2020-10-25