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[1]郭静,宋秀丽,白杰,等.氟磺胺草醚降解菌株TB-2的生物学特性及生物修复对玉米生长发育的影响[J].应用与环境生物学报,2020,26(05):1059-1065.[doi: 10.19675/j.cnki.1006-687x.2019.09025]
　GUO Jing,SONG Xiuli,BAI Jie & TAO Bo.Biological characteristics of fomesafen-degrading strain TB-2 and effects of bioremediation on growth and development of corn[J].Chinese Journal of Applied & Environmental Biology,2020,26(05):1059-1065.[doi: 10.19675/j.cnki.1006-687x.2019.09025]
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氟磺胺草醚降解菌株TB-2的生物学特性及生物修复对玉米生长发育的影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
26卷
期数:
2020年05期
页码:
1059-1065
栏目:
土壤与农业微生物应用专栏
出版日期:
2020-10-25

文章信息/Info

Title:
Biological characteristics of fomesafen-degrading strain TB-2 and effects of bioremediation on growth and development of corn
作者:
郭静; 宋秀丽; 白杰; 陶波
1东北农业大学农学院 哈尔滨 150030 2岭南师范学院 湛江 524048
Author(s):
GUO Jing1;  SONG Xiuli2;  BAI Jie1 & TAO Bo1?
1 College of Agriculture, Northeast Agricultural University, Harbin 150030, China 2 Lingnan Normal University, Zhanjiang 524048, China
关键词:
氟磺胺草醚; 残留药害; 假单胞杆菌; 生物修复; 玉米
Keywords:
fomesafen;  residual phytotoxicity;  Pseudomonas;  bioremediation;  corn
DOI:
10.19675/j.cnki.1006-687x.2019.09025
摘要:
为解决氟磺胺草醚残留药害问题，利用已筛选出的高效降解菌株假单胞杆菌TB-2，结合高效液相色谱法检测其残留浓度，统计分析计算其降解率，利用室内盆栽培养结合田间应用进行降解菌对玉米的生物修复研究. 结果显示，氟磺胺草醚残留浓度在0.1-10 mg/L范围内，标准曲线线性良好，符合农残分析要求；LB液体培养基中氟磺胺草醚在TB-2降解菌处理72 h的降解率接近96%；最适接种量是5%，最适葡萄糖含量0.5%，35 ℃、pH 7.0时菌株生长最佳. 盆栽试验土壤中氟磺胺草醚残留浓度为0.4 mg/kg时，加入降解菌后能够明显恢复玉米的各项生长指标和生理指标. 在田间应用中，降解菌对375 g a.i./hm2氟磺胺草醚污染的土壤修复作用明显. 不同剂型降解菌剂对玉米抽穗期的效果最为显著，能有效地减轻玉米毒害，促进玉米生长，减少产量损失. 在高剂量氟磺胺草醚处理下，降解菌剂应用效果依次为粒剂＞粉剂＞菌液. 本研究表明微生物修复可减轻氟磺胺草醚残留对后茬作物玉米的毒害作用，结果可为农药残留的生物修复提供科学参考. （图6 表2 参31）
Abstract:
To solve the toxicity problems associated with fomesafen residues, a highly efficient fomesafen-degrading TB-2 (Pseudomonas) strain was selected in the laboratory. The residual concentration of fomesafen was determined by high-performance liquid chromatography, and the degradation rate was calculated using statistical analysis. The bioremediation effect by degrading bacteria was carried out using a potted culture experiment combined with field applications. When the fomesafen residue concentration was in the range of 0.1–10 mg/L, the standard curve met the requirements of pesticide residue analysis. The study showed that the degradation rate of fomesafen by strain TB-2 was close to 96% within 72 h of incubation, the optimum inoculum size was found to be 5%, and the optimum glucose content was measured at 0.5%. The optimum temperature and pH were 35 ℃ and 7.0, respectively. When the residual concentration of fomesafen was 0.4 mg/kg, the growth index and physiological index of corn could be restored after adding degrading bacteria in the potted experiment. In field applications, the degrading bacteria were found to have a significant effect on the soil remediation of 375 g a.i./hm2 fomesafen. The different dosage forms of degrading bacteria had the most significant effect on the heading stage of corn, which can effectively alleviate the poisoning of corn, promote the growth of corn, and reduce the loss of yield. Under the high-dose fomesafen treatment, the application effect of the degrading bacteriostatic agent was found to be granules > powder > bacterial liquid. This study showed that microbial remediation could reduce the toxic effect of fomesafen residue on corn and has provided a scientific reference for bioremediation of pesticide residue.

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更新日期/Last Update: 2020-10-25