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[1]杜倩,赵文龙,李友国.基于根瘤菌来源的ACC脱氨酶基因提高大豆和紫云英根瘤菌的竞争结瘤能力[J].应用与环境生物学报,2020,26(01):88-95.[doi:10.19675/j.cnki.1006-687x.2019.04035]
 DU Qian,ZHAO Wenlong & LI Youguo.Improvement of competitive nodulation ability of Mesorhizobium huakuii and Sinorhizobium fredii based on the ACC deaminase gene of Bradyrhizobia[J].Chinese Journal of Applied & Environmental Biology,2020,26(01):88-95.[doi:10.19675/j.cnki.1006-687x.2019.04035]
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基于根瘤菌来源的ACC脱氨酶基因提高大豆和紫云英根瘤菌的竞争结瘤能力()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
26卷
期数:
2020年01期
页码:
88-95
栏目:
研究论文
出版日期:
2020-02-25

文章信息/Info

Title:
Improvement of competitive nodulation ability of Mesorhizobium huakuii and Sinorhizobium fredii based on the ACC deaminase gene of Bradyrhizobia
作者:
杜倩 赵文龙 李友国
华中农业大学生命科学技术学院,农业微生物学国家重点实验室 武汉 430070
Author(s):
DU Qian ZHAO Wenlong & LI Youguo?
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
关键词:
ACC脱氨酶华癸根瘤菌大豆根瘤菌竞争结瘤共生固氮
Keywords:
ACC deaminase Mesorhizobium huakuii Sinorhizobium fredii competitive nodulation symbiotic nitrogen fixation
DOI:
10.19675/j.cnki.1006-687x.2019.04035
摘要:
根瘤菌的竞争结瘤能力提高对提高接种剂的应用效果至关重要. 为提高根瘤菌的共生固氮和竞争结瘤能力,从大豆慢生型根瘤菌USDA110中扩增1-氨基环丙烷-1-羧酸脱氨酶(ACC脱氨酶)基因和其上游调控序列,通过两亲本接合转移导入华癸中慢生根瘤菌7653R、大豆快生型根瘤菌HH103和SMH12,构建含有ACC脱氨酶的重组根瘤菌. 结果显示:在土壤盆栽条件下,接种导入ACC脱氨酶的重组菌7653R,宿主植物地上鲜重、瘤数、瘤重和固氮酶活均显著增加,竞争结瘤能力提高22.6%;接种重组菌HH103,植物地上鲜重、瘤重和酶活显著增加,瘤数增加,竞争结瘤能力提高28.9%;接种重组菌SMH12,植物地上鲜重、瘤数、瘤重和酶活增加,未到达显著性,但竞争结瘤能力提高25.4%. 本研究表明构建的3个重组根瘤菌具有良好的共生固氮和竞争结瘤能力,可作为有潜力的优良菌株应用到田间试验. (图10 表2 参32)
Abstract:
The competitive nodulation ability of rhizobium is essential for improving the application effect of inoculants. In this study, to improve the symbiotic nitrogen fixation and competitive nodulation ability of rhizobium, the 1-aminocycopropane-1-carboxylate (ACC) deaminase gene and its upstream regulatory sequences from Bradyrhizobium japonicum USDA110 were introduced into Mesorhizobium huakuii 7653R, Sinorhizobium fredii HH103, and SMH12 to construct a recombinant rhizobia containing ACC deaminase using the two parental conjugations. Under soil potting conditions, the results showed that astragalus inoculated with recombinant 7653R containing ACC deaminase significantly increased the fresh weight of the aerial part, nodule number, nodule weight, and nitrogenase activity. The competitive nodulation ability was increased by 22.6%. In soybean inoculated with recombinant HH103, there was a significant increase in the fresh weight of the aerial part, nodule weight, nitrogenase activity, and nodule number. The competitive nodulation ability was increased by 28.9%. Inoculation with recombinant SMH12 increased the fresh weight of the aerial part, nodule number, nodule weight, and nitrogenase activity. There was no significant increase; however, the competitive nodulation ability was increased by 25.4%. The three recombinant rhizobia strains in this study have good symbiotic nitrogen fixation and competitive nodulation ability and can be applied in field trials as potentially excellent strains.

参考文献/References:

1 曾小红, 马焕成, 伍建榕, 郭瑞超, 肖斌, 张丽芬. 根瘤菌的抗旱性研究进展[J]. 西南林学院学报, 2005, 25 (3): 82-85 [Zeng XH, Mang HC, Wu JR, Guo RC, Xiao B, Zhang LF. Research advances in drought-resistance of rhizobium [J]. J S For Coll, 2005, 25 (3): 82-85]
2 Unay J, Perret X. Synthetic plasmids to challenge symbiotic nitrogen fixation between rhizobia and legumes [M]//Reinhardt D, Sharma A K. Methods in Rhizosphere Biology Research. Singapore: Springer Singapore, 2019: 3-18
3 Alves BJR, Boddey RM, Urquiaga S. The success of BNF in soybean in Brazil [J]. Plant Soil, 2003, 252 (1): 1-9
4 Hungria M, Mendes IC. Nitrogen Fixation with Soybean: the Perfect Symbiosis [M]. Hoboken: John Wiley & Sons Inc, 2015: 1009-1024
5 卢林纲. 黑龙江省大豆根瘤菌复合颗粒肥的研制及其应用技术研究[D]. 北京: 中国农业大学, 2005 [Lu LG. Developing Bradyrhizobium japonicum mixed pellet fertilizer and its application in Heilongjiang province [D]. Beijing: China Agricultural University, 2005]
6 李欣欣, 许锐能, 廖红. 大豆共生固氮在农业减肥增效中的贡献及应用潜[J]. 大豆科学, 2016, 35 (4): 531-535 [Li XX, Xu RN, Liao H. Contributions of symbiotic nitrogen fixation in soybean to reducing fertilization while increasing efficiency in agriculture [J]. Soybean Sci, 2016, 35 (4): 531-535]
7 何庆元, 胡艳, 玉永雄. 生态环境对根瘤菌竞争结瘤影响的研究进展[J]. 大豆科学, 2004, 23 (1): 66-70 [He QY, Hu Y, Yu YX. Advance on the study of the nodulation competition of rhizobium in environment [J]. Soybean Sci, 2004, 23 (1): 66-70]
8 钟喆栋, 曾小波, 李友国. ACC脱氨酶对大豆快生根瘤菌及苜蓿中华根瘤菌共生固氮与竞争结瘤的影响[J]. 华中农业大学学报, 2019, 38 (1): 28-34 [Zhong ZD, Zeng XB, Li YG. Effects of ACC deaminase on symbiotic nitrogen fixation and competitive nodulation in Sinorhizobium meliloti 1021 and Sinorhizobium fredii HH103 [J]. J Huazhong Agric Univ, 2019, 38 (1): 28-34]
9 Nukui N, Ezura H, Yuhashi K, Yasuta T, Minamisawa K. Effects of ethylene precursor and inhibitors for ethylene biosynthesis and perception on nodulation in Lotus japonicus and Macroptilium atropurpureum [J]. Plant Cell Physiol, 2000, 41 (7): 893-897
10 Oldroyd GE, Engstrom EM, Long SR. Ethylene inhibits the Nod factor signal transduction pathway of Medicago truncatula [J]. Plant Cell, 2001, 13 (8): 1835-1849
11 Nascimento FX, Brígido C, Glick BR, Rossi MJ. The role of rhizobial ACC deaminase in the nodulation process of leguminous plants [J]. Int J Agron, 2016, 2016: 1-9
12 Honma M. Stereospecific reation of 1-aminocyclopropane-1-carboxylate deaminase [M]//Cellular and Molecular Aspects of the Plant Hormone Ethylene. Dordrecht: Kluwer Academic, 1993, 111-116
13 Nascimento FX, Rossi MJ, Soares CRFS, Mcconkey BJ, Glick BR. New insights into 1-aminocyclopropane-1-carboxylate (ACC) deaminase phylogeny, evolution and ecological significance [J]. Plos One, 2014, 9 (6): e99168
14 Glick BR, Penrose DM, Li J. A model for the lowering of plant ethylene concentrations by plant growth-promoting bacteria [J]. J Theor Biol, 1998, 190 (1): 63-68
15 Khalid A, Ahmad Z, Mahmood S, Mahmood T, Imran M. Role of ethylene and bacterial ACC-deaminase in nodulation of legumes [M]//Zaidi A, Khan MS, Musarrat J. Microbes for Legume Improvement. Cham: Springer International Publishing, 2017: 95-118
16 Tittabutr P, Sripakdi S, Boonkerd N, Tanthanuch W, Minamisawa K, Teaumroong N. Possible role of 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity of Sinorhizobium sp. [J]. Microbes Environ, 2015, 30 (4): 310-320
17 Glick BR. Modulation of plant ethylene levels by the bacterial enzyme ACC deaminase [J]. Fems Microbiol Lett, 2005, 251 (1): 1-7
18 Nascimento FX, Brígido C, Glick BR, Oliveira S, Alho L. Mesorhizobium ciceri LMS-1 expressing an exogenous 1-aminocyclopropane-1-carboxylate (ACC) deaminase increases its nodulation abilities and chickpea plant resistance to soil constraints [J]. Lett Appl Microbiol, 2012, 55 (1): 15-21
19 Ma W, Charles TC, Glick BR. Expression of an exogenous 1-aminocyclopropane-1-carboxylate deaminase gene in sinorhizobium meliloti increases its ability to nodulate alfalfa [J]. Appl Environ Microb, 2004, 70 (10): 5891-5897
20 Bradford MM. A rapid and sensitive method for the quantitation quantities microgram principle of protein-dye binding [J]. Anal Biochem, 1976, 72: 248-254
21 Honma M, Shimomura T. Metabolism of 1-aminocyclopropane-1-carboxylic acid [J]. Agric Biol Chem, 1978, 42 (10): 1825-1831
22 Penrose DM, Glick BR. Methods for isolating and characterizing ACC deaminase-containing plant growth-promoting rhizobacteria [J]. Physiol Plant, 2003, 118 (1): 10-15
23 王金生, 丁宁, 吴俊江, 刘庆莉, 张鑫. 大豆根瘤菌接种效应及竞争结瘤能力分析[J]. 大豆科学, 2017, 36 (5): 761-767 [Wang JS, Ding N, Wu JJ, Liu QL, Zhang X. Analysis of the inoculation effect of soybean rhizobia and the competitive nodulation ability [J]. Soybean Sci, 2017, 36 (5): 761-767]
24 李剑峰, 张淑卿, 杜建雄, 韩晗, 张丽丽. 根瘤菌剂现存问题初探[J]. 微生物前言, 2015, 4 (1): 20-25 [Li JF, Zhang SQ, Du JX, Han H, Zhang LL. Preliminary discussion on problems of rhizobium inoculants [J]. Adv Microbiol, 2015, 4 (1): 20-25]
25 Bogino P, Banchio E, Bonfiglio C, Giordano W. Competitiveness of a Bradyrhizobium sp. strain in soils containing indigenous rhizobia [J]. Curr Microbiol, 2008, 56 (1): 66-72
26 Sanz-Sáez ?, Heath KD, Burke PV, Ainsworth EA. Inoculation with an enhanced N2-fixing Bradyrhizobium japonicum strain (USDA110) does not alter soybean (Glycine max Merr.) response to elevated [CO2] [J]. Plant Cell Environ, 2015, 38 (12): 2589-2602
27 Tavares MJ, Nascimento FX, Glick BR, Rossi MJ. The expression of an exogenous ACC deaminase by the endophyte Serratia grimesii BXF1 promotes the early nodulation and growth of common bean [J]. Lett Appl Microbiol, 2018, 66 (3): 252-259
28 Gontia-Mishra I, Sapre S, Kachare S, Tiwari S. Molecular diversity of 1-aminocyclopropane-1-carboxylate (ACC) deaminase producing PGPR from wheat (Triticum aestivum L.) rhizosphere [J]. Plant Soil, 2017, 414 (1-2): 213-227
29 Nascimento F, Brígido C, Alho L, Glick BR, Oliveira S. Enhanced chickpea growth-promotion ability of a Mesorhizobium strain expressing an exogenous ACC deaminase gene [J]. Plant Soil, 2012, 353 (1-2): 221-230
30 Nascimento FX, Tavares MJ, Glick BR, Rossi MJ. Improvement of Cupriavidus taiwanensis nodulation and plant growth promoting abilities by the expression of an exogenous ACC deaminase gene [J]. Curr Microbiol, 2018, 75 (8): 961-965
31 Conforte VP, Echeverria M, Sanchez C, Ugalde RA, Menendez B, Lepek VC. Engineered ACC deaminase-expressing free-living cells of Mesorhizobium loti show increased nodulation efficiency and competitiveness on Lotus spp. [J]. J Gen Appl Microbiol, 2010, 56 (4): 331-338
32 戴小密, 刘彦杰, 叶小梅, 朱万宝, 许玲, 朱家壁, 常志州, 黄红英, 马艳, 俞冠翘. 接种大豆根瘤菌(Sinorhizobium fredii)遗传工程菌株LMG101对大豆的增产效应[J]. 中国农业科学, 2003, 36 (1): 66-70 [Dai XM, Liu YJ, Ye XM, Zhu WB, Xu L, Zhu JB, Chang ZZ, Huang HY, Ma Y, Yu GQ. Soybean yield response to inoculation with genetically engineered strain LMG101 of Sinorhizobium fredii [J]. Sci Agric Sin, 2003, 36 (1): 66-70]

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更新日期/Last Update: 2020-02-25