|本期目录/Table of Contents|

[1]谈高维,韦布春,崔永亮,等.镉钝化细菌对水稻幼苗镉吸收的影响[J].应用与环境生物学报,2019,25(03):524-531.[doi:10.19675/j.cnki.1006-687x.201812006]
　TAN Gaowei,WEI Buchun,CUI Yongliang,et al.Effects of cadmium passivation bacteria on the growth and cadmium adsorption of rice seedlings[J].Chinese Journal of Applied & Environmental Biology,2019,25(03):524-531.[doi:10.19675/j.cnki.1006-687x.201812006]
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镉钝化细菌对水稻幼苗镉吸收的影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年03期
页码:
524-531
栏目:
重金属污染及生物修复专栏
出版日期:
2019-06-25

文章信息/Info

Title:
Effects of cadmium passivation bacteria on the growth and cadmium adsorption of rice seedlings
作者:
谈高维; 韦布春; 崔永亮; 陈香归; 闫敏; 沈甜; 秦诗洁; 羊鑫; 江鑫; 余秀梅
1四川农业大学资源学院 成都 611130 2四川省自然资源科学研究院 成都 610041
Author(s):
TAN Gaowei1;  WEI Buchun1;  CUI Yongliang2;  CHEN Xianggui1;  YAN Min1;  SHEN Tian1;  QIN Shijie1;  YANG Xin1;  JIANG Xin1 & YU Xiumei1**
1 College of Resources, Sichuan Agricultural University, Chengdu 611130, China; 2 Natural Resources Research Institute of Sichuan Province, Chengdu 610041, China
关键词:
镉; 钝化微生物; 水稻幼苗; 促生
Keywords:
cadmium;  passivation;  bacteria;  rice seedling;  growth promoting
分类号:
X172 : X53
DOI:
10.19675/j.cnki.1006-687x.201812006
摘要:
为了解根际重金属钝化微生物对作物生长及土壤重金属吸收的影响，将来源于镉（Cd）污染水稻土的镉钝化细菌枯草芽孢杆菌CDR-1、雷氏普罗威登斯菌CDR-2、阿耶波多氏芽孢杆菌CDR-3分别接种到水稻幼苗根际，通过测定水稻苗的生长、生物量及镉含量来分析根际镉钝化细菌对水稻生长及镉吸收的影响. 结果显示，镉钝化细菌CDR-1、CDR-2、CDR-3的Cd2+最低抑制浓度分别为200、200、400 mg/L，其在20 mg/L Cd2+溶液中的镉钝化率均达到100%，且随Cd2+浓度的上升镉钝化率呈现下降趋势. 在5和10 mg/L Cd2+胁迫下，这3株细菌对水稻幼苗的生长有不同程度的影响，并都能显著降低水稻幼苗根和地上部分的镉含量，其中 CDR-1对水稻幼苗的促生降镉作用最优；在0.5、1和2 mg/kg Cd2+胁迫下，CDR-1仍然能显著促进水稻幼苗生长，增加水稻幼苗生物量，并能使水稻幼苗根和地上部分的镉含量降低34.88%-61.63%. 本研究表明Cd2+钝化细菌能够显著降低水稻幼苗的镉吸收量，Cd2+钝化促生细菌CDR-1可应用于镉污染农田土壤的生物修复. （图4 表3 参30）
Abstract:
Whether heavy metal-passivating microorganisms affect crop growth and the uptake of soil heavy metals is not clear. This study focuses on the effects of cadmium (Cd)-resistant bacteria on the growth of and Cd uptake in rice seedlings under Cd stress. The cadmium-passivating bacteria, Bacillus subtilis CDR-1, Bacillus aryabhattai CDR-2, and Providencia rettgeri CDR-3, were derived from cadmium-contaminated paddy soil, inoculated into the rhizosphere of rice seedlings, and their effects on the growth and cadmium absorption of rice were analyzed by measuring the growth biomass and cadmium content of these rice seedlings. The lowest inhibition concentrations of Cd2+ in CDR-1, CDR-2, and CDR-3 were 200 mg/L, 200 mg/L, and 400 mg/L, respectively. Their Cd2+ passivation rates were 100% in a 20 mg/L Cd2+ solution. The Cd2+ passivation rate decreased with increase in Cd2+ concentration. Under 5 mg/L and 10 mg/L of Cd2+ stress, the three bacterial strains exhibited different degrees of effects on the growth of rice seedlings, and all of them could significantly reduce the cadmium content in the root and over-the-ground parts of the rice seedlings. Among the three strains of bacteria, the role of CDR-1 in promoting growth and decreasing cadmium content in rice seedlings is the best. Under 0.5 mg/kg, 1 mg/kg, and 2 mg/kg of Cd2+ stress, CDR-1 can still significantly promote growth, increase the biomass, and reduce the content of cadmium in the root and over-the-ground parts of rice seedlings by 34.88%-61.63%. This study showed that the Cd2+-passivating bacteria can significantly reduce cadmium uptake in rice seedlings, and the Cd2+ passivating and growth promoting bacteria CDR-1 can be used for the bioremediation of cadmium-contaminated farmland soil.

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更新日期/Last Update: 2019-06-25