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[1]叶婷,刘亚男,王兴香,等.氯化汞对大鲵心脏和胰腺CYP2亚家族成员CYP2K1、CYP2F3、CYP2F5和CYP2C19基因表达的影响[J].应用与环境生物学报,2019,25(03):546-552.[doi:10.19675/j.cnki.1006-687x.201807025]
　YE Ting,LIU Yanan,WANG Xingxiang,et al.Effects of mercuric chloride on the expression of the CYP2K1, CYP2F3, CYP2F5, and CYP2C19 genes in the CYP2 subfamily in the heart and pancreas of the* Chinese giant salamander, Andrias davidianus[J].Chinese Journal of Applied & Environmental Biology,2019,25(03):546-552.[doi:10.19675/j.cnki.1006-687x.201807025]
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氯化汞对大鲵心脏和胰腺CYP2亚家族成员CYP2K1、CYP2F3、CYP2F5和CYP2C19基因表达的影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年03期
页码:
546-552
栏目:
重金属污染及生物修复专栏
出版日期:
2019-06-25

文章信息/Info

Title:
Effects of mercuric chloride on the expression of the CYP2K1, CYP2F3, CYP2F5, and CYP2C19 genes in the CYP2 subfamily in the heart and pancreas of the* Chinese giant salamander, Andrias davidianus
作者:
叶婷; 刘亚男; 王兴香; 曹宇; 许抗抗; 李灿
贵阳学院生物与环境工程学院，贵州省山地珍稀动物与经济昆虫重点实验室 贵阳 550005
Author(s):
YE Ting;  LIU Ya’nan;  WANG Xingxiang;  CAO Yu;  XU Kangkang & LI Can**
College of Biology and Environmental Engineering, Guiyang University / Guizhou Provincial Key Laboratory for Rare Animal and Economic Insects of the Mountainous Region, Guiyang 550005, China
关键词:
大鲵; 氯化汞; 细胞色素P450 2K1（CYP2K1）; 细胞色素P450 2F3（CYP2F3）; 细胞色素P450 2F5（CYP2F5）; 细胞色素P450 2C19（CYP2C19）
Keywords:
Andrias davidianus;  mercuric chloride;  cytochrome P450 2K1 (CYP2K1);  cytochrome P450 2F3 (CYP2F3);  cytochrome P450 2F5 (CYP2F5);  cytochrome P450 2C19 (CYP2C19)
分类号:
X174: X171.5
DOI:
10.19675/j.cnki.1006-687x.201807025
摘要:
探究氯化汞（HgCl2）对大鲵心脏和胰腺细胞色素P450 2（CYP2）亚家族成员细胞色素P450 2K1（CYP2K1）、P450 2F3（CYP2F3）、P450 2F5（CYP2F5）和P450 2C19（CYP2C19）基因表达的影响，可为评价HgCl2对大鲵的生态毒理影响提供基础数据. 幼龄大鲵暴露于HgCl2（1、10、100 ng/L）24、48、72 h后，采用qRT-PCR试验分析HgCl2处理不同时间后CYP2亚家族成员CYP2K1、CYP2F3、CYP2F5和CYP2C19在心脏和胰腺中的表达变化模式. 结果显示：HgCl2对大鲵暴露24 h后，大鲵心脏CYP2K1、CYP2F3、CYP2F5和CYP2C19基因表达水平相对于对照组均呈现上升变化趋势，即1 ng/L HgCl2显著诱导了大鲵心脏CYP2K1和CYP2C19基因表达水平（P < 0.05），10 ng/L HgCl2显著诱导了大鲵心脏CYP2F3和CYP2F5基因表达水平（P < 0.05），100 ng/L HgCl2均显著诱导了大鲵心脏CYP2K1、CYP2F3、CYP2F5和CYP2C19基因表达（P < 0.05）；大鲵胰腺CYP2K1、CYP2F3、CYP2F5和CYP2C19基因表达水平相对于对照组均呈现下降变化趋势，10、100 ng/L HgCl2均显著抑制了大鲵胰腺CYP2K1、CYP2F3、CYP2F5和CYP2C19基因表达（P < 0.05）. HgCl2对大鲵暴露48 h后，大鲵心脏CYP2F5基因表达水平相对于对照组均呈现显著下降变化趋势（P < 0.05），并具有浓度梯度效应；大鲵胰腺CYP2F3基因表达水平受到显著诱导（P < 0.05）. HgCl2对大鲵暴露72 h后，与对照相比大鲵心脏和胰腺CYP2K1、CYP2F3、CYP2F5和CYP2C19基因表达均无显著性变化. 本研究表明随着HgCl2暴露时间延长，CYP2亚家族基因表达无显著变化，大鲵心脏和胰腺CYP2亚家族基因CYP2K1、CYP2F3、CYP2F5和CYP2C19表达对HgCl2刺激转变为慢性累积毒性效应；结果可为评价HgCl2对大鲵的生态毒理影响提供基础数据. （图2 表1 参33）
Abstract:
The effects of mercuric chloride (HgCl2) on the expression of the cytochrome P450 2K1 (CYP2K1), cytochrome P450 2F3 (CYP2F3), cytochrome P450 2F5 (CYP2F5), and cytochrome P450 2C19 (CYP2C19) genes in the cytochrome P450 2 (CYP2) subfamily in the heart and pancreas of the Chinese giant salamander, Andrias davidianus, were investigated in this study to provide basic data for evaluating the ecotoxicological effects of mercuric chloride on this species. Juvenile giant salamanders were continuously exposed to different concentrations of HgCl2 (0, 1, 10, and 100 ng/L) for 24, 48, and 72 h, and the effects of mercuric chloride on the expression of the CYP2K1, CYP2F3, CYP2F5, and CYP2C19 genes in their heart and pancreas were investigated using real-time quantitative reverse transcriptase polymerase chain reaction (RT-qPCR). After exposure to HgCl2 for 24 h, the expression levels of the CYP2K1, CYP2F3, CYP2F5, and CYP2C19 genes in the hearts of giant salamanders were increased compared with those in the control group (exposed to 0 ng/L HgCl2). In the hearts of giant salamanders exposed to 1 ng/L HgCl2 for 24 h, the expression levels of the CYP2K1 and CYP2C19 genes were significantly increased (P < 0.05), while exposure to 10 ng/L HgCl2 for 24 h significantly increased CYP2F3 and CYP2F5 gene expression levels (P < 0.05), and CYP2K1, CYP2F3, CYP2F5, and CYP2C19 gene expression levels were all significantly increased by exposure to 100 ng/L HgCl2 for 24 h (P < 0.05). In the pancreases of giant salamanders, the expression levels of the CYP2K1, CYP2F3, CYP2F5, and CYP2C19 genes tended to be lower than those in the control group after exposure to HgCl2 for 24 h. Exposure to both 10 and 100 ng/L HgCl2 for 24 h significantly inhibited the expression of the CYP2K1, CYP2F3, CYP2F5, and CYP2C19 genes in the pancreas (P < 0.05). After exposure to HgCl2 for 48 h, the expression levels of CYP2F5 in the hearts of giant salamanders significantly decreased in a dose-dependent manner (P < 0.05), and significantly elevated CYP2F3 expression was found in the pancreas (P < 0.05). After exposure to HgCl2 for 72 h, there were no significant changes in the expression levels of the CYP2K1, CYP2F3, CYP2F5, and CYP2C19 genes in the hearts and pancreases of giant salamanders compared with those in the control treatment (P > 0.05). It was concluded that no significant change in the expression of CYP2 subfamily genes occurred with increasing exposure time, which suggested that the responses of the CYP2 subfamily genes CYP2K1, CYP2F3, CYP2F5, and CYP2C19 in the heart and pancreas of the Chinese giant salamander may be converted into a chronic cumulative toxic effect in response to exposure to mercuric chloride exposure.

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更新日期/Last Update: 2019-06-25