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[1]范延芬,魏春梅,李彩侠 王 蕾 李鑫雨 马欣荣**.光能变价离子钛对多种细菌的抑制作用[J].应用与环境生物学报,2016,22(06):1150-1155.[doi:10.3724/SP.J.1145.2016.06016]
　FAN Yanfen,WEI Chunmei,et al.Antimicrobial activity of titanium ion of variable valence with light energy[J].Chinese Journal of Applied & Environmental Biology,2016,22(06):1150-1155.[doi:10.3724/SP.J.1145.2016.06016]
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光能变价离子钛对多种细菌的抑制作用()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
22卷
期数:
2016年06期
页码:
1150-1155
栏目:
研究论文
出版日期:
2016-12-25

文章信息/Info

Title:
Antimicrobial activity of titanium ion of variable valence with light energy
作者:
范延芬1;  2 魏春梅1;  2 李彩侠1 王 蕾3 李鑫雨3 马欣荣1**
1中国科学院成都生物研究所 成都 610041 2中国科学院大学 北京 100049 3四川师范大学生命科学学院 成都 610101
Author(s):
FAN Yanfen1;  2;  WEI Chunmei1;  2;  LI Caixia1;  WANG Lei3;  LI Xinyu3;  MA Xinrong1**
1Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China 2University of Chinese Academy of Sciences, Beijing 100049, China 3College of Life Sciences, Sichuan Normal University, Chengdu 610101, China
关键词:
光能变价离子钛; 细菌; 植物致病细菌; 抑菌活性; 农药减施
Keywords:
titanium ion of variable valence with light energy (TIVL);  bacteria;  plant-pathogenic bacteria;  antibacterial activity;  bactericide reduction
分类号:
S482.2
DOI:
10.3724/SP.J.1145.2016.06016
摘要:
光能变价离子钛（Titanium ion of variable valence with light energy，TIVL）对环境友好、对人畜无害，能有效减少作物的发病率及发病症状. 为了解其抑菌活性，采用不同浓度的TIVL对5种细菌，大肠杆菌（Escherichia coli）、娄彻氏链霉菌（Streptomyces rochei）、根癌农杆菌（Agrobacterium tumefaciens）、成团泛菌（Pantoe aagglomerans）和茄科雷尔氏菌（Ralstonia solanacearum），进行平板抑菌试验研究. 结果显示，在一定菌液浓度（2 × 103 - 104 CFU/mL）下，16 mg/L的TIVL即可显著抑制这5种菌绝大部分菌落的定植和生长. TIVL浓度为4 mg/L时，即可有效抑制娄彻氏链霉菌，抑菌率达97.33%；浓度为8 mg/L时，99.15%大肠杆菌和89.16%根癌农杆菌被抑制；浓度为16 mg/L时，87.68%成团泛菌和74.06%茄科雷尔氏菌被抑制. 结果表明TIVL在低浓度下即对上述5种菌有良好的抑制作用，推测TIVL具有广谱抑菌活性；本研究可为进一步研究TIVL抑菌机理及农业生产应用提供依据，为农药减施、绿色农作物产品生产提供新途径. （图10 表1 参26）
Abstract:
The overuse of agrochemicals in our country over the years has caused serious environmental problems and brought in an increasing concern on agricultural product quality and safety. Titanium ion of variable valence with light energy (TIVL), an environmentally friendly reagent, could effectively reduce the incidences of various crop diseases and alleviate diseases symptoms caused by pathogens. However, its bacteriostatic activity has not yet been studied. In this research, the plate smearing method was utilized to investigate the bacteriostatic activity of TIVL against Escherichia coli, Streptomyces rochei, Agrobacterium tumefaciens, Pantoe agglomerans and Ralstonia solanacearum. The results showed that 16 mg/L TIVL significantly inhibited bacterial colonization and growth (at a certain concentration of bacteria 2 × 103 - 104 CFU/mL). At the concentration of 4 mg/L, TIVL effectively inhibited 97.33% S. rochei, and at 8 mg/L, it inhibited 99.15% E. coli and 89.16% A. tumefacien, while at 16 mg/L, it suppressed P. agglomerans and R. solanacearum at 87.68% and 74.06% respectively. The results exhibited that TIVL has a significant inhibitory effect on multiple strains of bacteria at low concentrations which suggested TIVL had a broad spectrum of bacteriostatic activity. This study provides a basis for further research on the antibacterial mechanism and application of TIVL, and indicates that TIVL can potentially be a new environmentally friendly bactericide for crop cultivation.

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更新日期/Last Update: 2016-12-30