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[1]范延芬,魏春梅,李彩侠 王 蕾 李鑫雨 马欣荣**.光能变价离子钛对多种细菌的抑制作用[J].应用与环境生物学报,2016,22(06):1150-1155.[doi:10.3724/SP.J.1145.2016.06016]
　FAN Yanfen,WEI Chunmei,et al.Antimicrobial activity of titanium ion of variable valence with light energy[J].Chinese Journal of Applied & Environmental Biology,2016,22(06):1150-1155.[doi:10.3724/SP.J.1145.2016.06016]
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光能变价离子钛对多种细菌的抑制作用()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
22卷
期数:
2016年06期
页码:
1150-1155
栏目:
研究论文
出版日期:
2016-12-25

文章信息/Info

Title:
Antimicrobial activity of titanium ion of variable valence with light energy
作者:
范延芬1;  2 魏春梅1;  2 李彩侠1 王 蕾3 李鑫雨3 马欣荣1**
1中国科学院成都生物研究所 成都 610041 2中国科学院大学 北京 100049 3四川师范大学生命科学学院 成都 610101
Author(s):
FAN Yanfen1;  2;  WEI Chunmei1;  2;  LI Caixia1;  WANG Lei3;  LI Xinyu3;  MA Xinrong1**
1Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China 2University of Chinese Academy of Sciences, Beijing 100049, China 3College of Life Sciences, Sichuan Normal University, Chengdu 610101, China
关键词:
光能变价离子钛; 细菌; 植物致病细菌; 抑菌活性; 农药减施
Keywords:
titanium ion of variable valence with light energy (TIVL);  bacteria;  plant-pathogenic bacteria;  antibacterial activity;  bactericide reduction
分类号:
S482.2
DOI:
10.3724/SP.J.1145.2016.06016
摘要:
光能变价离子钛（Titanium ion of variable valence with light energy，TIVL）对环境友好、对人畜无害，能有效减少作物的发病率及发病症状. 为了解其抑菌活性，采用不同浓度的TIVL对5种细菌，大肠杆菌（Escherichia coli）、娄彻氏链霉菌（Streptomyces rochei）、根癌农杆菌（Agrobacterium tumefaciens）、成团泛菌（Pantoe aagglomerans）和茄科雷尔氏菌（Ralstonia solanacearum），进行平板抑菌试验研究. 结果显示，在一定菌液浓度（2 × 103 - 104 CFU/mL）下，16 mg/L的TIVL即可显著抑制这5种菌绝大部分菌落的定植和生长. TIVL浓度为4 mg/L时，即可有效抑制娄彻氏链霉菌，抑菌率达97.33%；浓度为8 mg/L时，99.15%大肠杆菌和89.16%根癌农杆菌被抑制；浓度为16 mg/L时，87.68%成团泛菌和74.06%茄科雷尔氏菌被抑制. 结果表明TIVL在低浓度下即对上述5种菌有良好的抑制作用，推测TIVL具有广谱抑菌活性；本研究可为进一步研究TIVL抑菌机理及农业生产应用提供依据，为农药减施、绿色农作物产品生产提供新途径. （图10 表1 参26）
Abstract:
The overuse of agrochemicals in our country over the years has caused serious environmental problems and brought in an increasing concern on agricultural product quality and safety. Titanium ion of variable valence with light energy (TIVL), an environmentally friendly reagent, could effectively reduce the incidences of various crop diseases and alleviate diseases symptoms caused by pathogens. However, its bacteriostatic activity has not yet been studied. In this research, the plate smearing method was utilized to investigate the bacteriostatic activity of TIVL against Escherichia coli, Streptomyces rochei, Agrobacterium tumefaciens, Pantoe agglomerans and Ralstonia solanacearum. The results showed that 16 mg/L TIVL significantly inhibited bacterial colonization and growth (at a certain concentration of bacteria 2 × 103 - 104 CFU/mL). At the concentration of 4 mg/L, TIVL effectively inhibited 97.33% S. rochei, and at 8 mg/L, it inhibited 99.15% E. coli and 89.16% A. tumefacien, while at 16 mg/L, it suppressed P. agglomerans and R. solanacearum at 87.68% and 74.06% respectively. The results exhibited that TIVL has a significant inhibitory effect on multiple strains of bacteria at low concentrations which suggested TIVL had a broad spectrum of bacteriostatic activity. This study provides a basis for further research on the antibacterial mechanism and application of TIVL, and indicates that TIVL can potentially be a new environmentally friendly bactericide for crop cultivation.

参考文献/References:

1 Zierden MR, Valentine AM. Contemplating a role for titanium in organisms [J]. Metallomics, 2016, 8 (1): 9-16 2 许国栋, 王桂生. 钛金属和钛产业的发展[J]. 稀有金属, 2009, 33 (6): 903-910 [Xu GD, Wang GS. Development of titanium and its industry [J]. Rare?Metals, 2009, 33 (6): 903-910] 3 刘志高, 张斌, 童再康, 季梦成, 梅婷. 钛离子注入对3种石蒜属植物种子生物化学特性的影响[J].?核农学报, 2013, 27 (2): 146-151 [Liu ZG, Zhang B, Dong ZK, Ji MC, Mei T. Effects of Ti + implantation on biological and chemical characteristics of seeds from three species of Lycoris [J]. J Nucl Agric Sci, 2013, 27 (2): 146-151] 4 曾艳玲, 谭晓风, 张斌, 范晓明, 刘长虹. 钛离子注入对油茶种子生理生化反应及初期生长的影响[J].?中国农学通报, 2011, 27 (28): 53-57 [Zeng YL, Tan XF, Zhang B, Fan XM, Liu CH. Effects of the Ti2+ ion implantation on physiological-biochemical reaction and seminal growth of Camellia Oleifera seeds [J]. Chin Agric Bull, 2011, 27 (28): 53-57] 5 范秀菊. 钛的氧化还原特性及其在植物生化过程中的作用[J]. 中国土壤与肥料, 2012, (5): 1-4 [Fan XJ. Redox properties of titanium and its action on biochemical process in plants [J]. China?Soils?Fert, 2012, (5): 1-4] 6 杜娟, 许自成, 李志刚, 苏永士, 陈彦春, 汪孝国. 植物钛素营养研究进展[J]. 江西农业学报, 2010, 22 (1): 42-44 [Du J, Xu ZC, Li ZG, Su YZ, Chen YC, Wang XG. Study progress in titanium nutrient of plants [J]. Acta Agric Jiangxi, 2010, 22 (1): 42-44] 7 李玲玲. 农作物施用钛化合物的适宜浓度[J]. 钛工业进展, 1998, 2: 45 8 巫维杰, 吴勇, 乔欣欣, 何密. 不同浓度抗水解稳定离子钛(ASIT)对3种高寒牧草种子发芽率的影响[J]. 南方农业, 2015, 9 (3): 24-25 9 徐伦, 张光跃. 抗水解稳定离子钛在大豆上的应用[J]. 西昌学院学报(自然科学版), 2010, 24 (2): 15-17 [Xu L, Zhang GY. The application of hydrolytic stable ions titanium in soybean [J]. J Xichang Coll (Nat Sci Ed), 2010, 24 (2): 15-17] 10 邢小军, 张建慧, 王勇. 离子钛对烟苗生长和烟叶产质量的影响[J]. 中国烟草科学, 2010, 31 (6): 38-40 [Xing XJ, Zhang JH, Wang Y. Effect of ionic titanium on seedling, yield and quality of flue-cured tobacco leaf [J]. Chin Tob Sci, 2010, 31 (6): 38-40] 11 Kalita A, Hu J, Torres, AG. Recent advances in adherence and invasion of?pathogenic?Escherichia?coli [J]. Curr Opin Infect Dis, 2014, 27 (5): 459-464 12 Grammatiki SK, Efstathios AK, Alexandros LS, Amalia DK. Streptomyces rochei ACTA1551, an indigenous Greek isolate studied as a potential biocontrol agent against Fusarium oxysporum f.sp. lycopersici [J]. Biomed Res Int, 2013, 10, ID 387230 13 项存涕, 宋福强, 柳京花, 董爱荣, 薛煜. 杨树冰核细菌溃疡病发病的主导因素[J]. 东北林业大学学报, 2001, 29 (3): 109-113 [Xiang CT, Song FQ, Liu JH, Dong AR, Xue Y. The dominant faetors caused by poplar INA baeterial canker [J]. J Northeast Agric Univ, 2001, 29 (3): 109-113] 14 孙富艳, 卢洪洲. 成团泛菌感染的研究近况[J]. 中国感染与化疗杂志, 2009, 9 (5): 389-391 [Sun FY, Lu HZ. Updates of Pantoea agglomerans infections [J]. Chin J Infec Chemoth, 2009, 9 (5): 389-391] 15 Bergman KA, Arends JP, Sch?lvinck EH. Pantoea agglomerans septicemia in three newborn infants [J]. Pediatr Infect Dis J, 2007, 26 (5): 453-454 16 宗晓福, 刘云霞, 王琴. 成团泛菌肺部感染8例[J] .中国医刊, 2007, 42 (8): 43-44 [Zong XF, Liu YX, Wang Q. The analysis of 8 cases with lung infection caused by P. agglomerans [J]. Chin J Med, 2007, 42 (8): 43-44] 17 Hayward AC. Biology and epidemiology of bacterial wilt caused by Pseudomonas solanacearum [J]. Annu Rev Phytopathol, 1991, 29: 65-87 18 李鹏, 吴毅歆, 毛自朝, 李琪彬, 何月秋. 青枯劳尔氏菌潜在新寄主鉴定与青枯病防治策略的思考[J]. 中国农学通报, 2013, 29 (6): 199-202 [Li P, Wu YX, Mao ZC, Li QB, He YQ. Identification of the potential new hosts of Ralstonia solanacearum and thinking of control strategies for bacterial wilt [J]. Chin Agric Sci Bull, 2013, 29 (6): 199-202] 19 Liou JW, Chang HH. Bactericidal effects and mechanisms of visible light-responsive titanium dioxide photocatalysts on pathogenic bacteria [J]. Arch Immunol Ther Exp (Warsz), 2012, 60 (4): 267-75 20 Leyland NS, Podporska-Carroll J, Browne J, Hinder SJ, Quilty B, Pillai SC. Highly efficient F, Cu doped TiO2 anti-bacterial visible light active photocatalytic coatings to combat hospital-acquired infections [J]. Sci Rep, 2016, 6: 24770 21 Yadav HM, Kim JS, Pawar SH. Developments in photocatalytic antibacterial activity of nano TiO2: a review [J]. Korean J Chem Eng, 2016, 33: 1989-1998 22 Lilja M, Forsgren J, Welch K, Astrand M, Engqvist H, Stromme M. Photocatalytic and antimicrobial properties of surgical implant coatings of titanium dioxide deposited though cathodic arc evaporation [J]. Biotechnol Lett, 2012, 34: 2299-2305 23 Suketa N, Sawase T, Kitaura H, Naito M, Baba K, Nakayama K, Wennerberg A, Atsuta M. An antibacterial surface on dental implants, based on the photocatalytic bactericidal effect [J]. Clin Implant Dent R, 2005, 7: 105-111 24 Nakamura H, Anaka M, Shinohara S, Gotoh M, Karube I. Development of a self-sterilizing lancet coated with a titanium dioxide photocatalytic nano-layer for self-monitoring of blood glucose [J]. Biosens Bioelectron, 2007, 22: 1920-1925 25 Yin ZF, Wu L, Yang HG, Su YH. Recent progress in biomedical applications of titanium dioxide [J]. Phys Chem Chem Phys, 2013, 15: 4844-4858 26 Vatansever F, de Melo WC, Avci P, Vecchio D, Sadasivam M, Gupta A, Chandran R, Karimi M, Parizotto NA, Yin R, Tegos GP, Hamblin MR. Antimicrobial strategies centered around reactive oxygen species-bactericidal antibiotics, photodynamic therapy and beyond [J]. FEMS Microbiol Rev, 2013, 37 (6): 955-989

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更新日期/Last Update: 2016-12-30