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[1]罗禹 周静 吴瑕 高平 袁小红.橙盖鹅膏菌中过氧麦角甾醇对人卵巢癌A2780S细胞增殖的影响[J].应用与环境生物学报,2016,22(06):1108-1112.[doi:10.3724/SP.J.1145.2016.01027]
 LUO Yu,ZHOU Jing,et al.The effect of ergosterol peroxide isolated from Amanita caesarea on the proliferation of ovarian cancer cells A2780S[J].Chinese Journal of Applied & Environmental Biology,2016,22(06):1108-1112.[doi:10.3724/SP.J.1145.2016.01027]
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橙盖鹅膏菌中过氧麦角甾醇对人卵巢癌A2780S细胞增殖的影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
22卷
期数:
2016年06期
页码:
1108-1112
栏目:
研究论文
出版日期:
2016-12-25

文章信息/Info

Title:
The effect of ergosterol peroxide isolated from Amanita caesarea on the proliferation of ovarian cancer cells A2780S
作者:
罗禹 周静 吴瑕 高平 袁小红
1四川大学生命科学学院药用天然产物实验室 成都 610015 2四川省中医药科学院 成都 610041 3西南科技大学生命科学与工程学院 绵阳 621010 4四川爱众生物医药科技有限公司 成都 610041
Author(s):
LUO Yu1 4 ZHOU Jing2 WU Xia2 GAO Ping1 & YUAN Xiaohong3**
1Laboratory of Medicinal Natural Products, College of Life Sciences, Sichuan University, Chengdu 610015, China 2Sichuan Academy of Traditional Chinese Medicine Sciences, Chengdu 610041, China 3School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China 4Sichuan Aizhong Biological Medicine Science and Technology Co., Ltd, Chengdu 610041, China
关键词:
过氧麦角甾醇A2780S细胞细胞增殖细胞凋亡细胞周期
Keywords:
ergosterol peroxide A2780S cell cell proliferation cell apoptosis cell cycle
分类号:
Q949.329.810.6 : R965
DOI:
10.3724/SP.J.1145.2016.01027
摘要:
为了解橙盖鹅膏菌中分离得到的过氧麦角甾醇对人卵巢癌A2780S细胞增殖的影响及其可能的机制,取对数生长期的A2780S细胞,经1.10、2.19、4.38、8.75、17.5、35 μmol/dL等浓度下的过氧麦角甾醇作用,以CCK-8法测试24、48和72 h后细胞增殖;经2.19、4.38、8.75 μmol/dL浓度的过氧麦角甾醇作用24 h后,倒置显微镜下观察细胞形态,DAPI染色法观察细胞核形态,Annexin V/PI双染流式细胞术测定细胞的早期凋亡率,PI单染流式细胞术测定细胞周期. 结果显示,过氧麦角甾醇作用于人卵巢癌A2780S细胞后,17.5 μmol/dL、35 μmol/dL浓度组48 h、72 h出现大量细胞死亡,贴壁存活细胞数目明显减少,细胞核呈现核浓缩的高量区域及月牙状的特征,细胞早期凋亡率、细胞S期比例显著增加. 本研究表明过氧麦角甾醇对A2780S细胞增殖具有明显抑制作用,可能与其阻滞细胞周期和诱导细胞凋亡有关. (图4 表3 参16)
Abstract:
This research aimed to study the proliferation of ovarian cancer cells A2780S induced by ergosterol peroxide isolated from Amanita caesarea. A2780S cells on the logarithmic growth phase were treated by ergosterol peroxide at the doses of 1.10, 2.19, 4.38, 8.75, 17.5 and 35 μmol/dL for 24, 48 and 72 h, and cell proliferation was determined by CCK-8 assay. After treatment at dose of 2.19, 4.38 and 8.75 μmol/dL for 24 h, the cell morphology was observed by inverted microscope and cell nuclear morphology was observed by DAPI assay. The early apoptosis rate of cells was determined by Annexin V/PI double-staining flow cytometry and the cell cycle was determined by PI cytometry. Ergosterol peroxide was found to inhibit the proliferation of A2780S. It increased the exfoliative and necrotic cells, and significantly increased the proportion of cells in S phase and early apoptosis of cells. The inhibition of A2780S cell proliferation by ergosterol peroxide may be through cell cycle blockage and cell apoptosis induction.

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更新日期/Last Update: 2016-12-30