|本期目录/Table of Contents|

[1]顾磊,许科伟,汤玉平,等.基于高通量测序技术研究玉北油田上方微生物多样性[J].应用与环境生物学报,2017,23(2):276-282.[doi:10.3724/SP.J.1145.2016.09027]
 GU Lei,XU Kewei,et al.Microbial diversity in Yubei Oil field determined by high-throughput sequencing[J].Chinese Journal of Applied & Environmental Biology,2017,23(2):276-282.[doi:10.3724/SP.J.1145.2016.09027]
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基于高通量测序技术研究玉北油田上方微生物多样性()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
23卷
期数:
2017年第2期
页码:
276-282
栏目:
研究论文
出版日期:
2017-04-25

文章信息/Info

Title:
Microbial diversity in Yubei Oil field determined by high-throughput sequencing
作者:
顾磊许科伟汤玉平杨帆孙永革
1浙江大学地球科学系 杭州 310027 2中国石油化工股份有限公司石油勘探开发研究院无锡石油地质研究所 无锡 214151
Author(s):
GU Lei1 2 XU Kewei2 TANG Yuping2 YANG Fan2 & SUN Yongge1**
1Department of Earth Science, Zhejiang University, Hangzhou 310027, China 2Wuxi Research Institute of Petroleum Geology, Research Institute of Petroleum Exploration & Production, SINOPEC, Wuxi 214151, China
关键词:
油气微生物勘探高通量测序群落结构微生物多样性
Keywords:
microbial prospecting for oil and gas high-throughput sequencing community structure microbial diversity
分类号:
Q939.99 : TE357.9
DOI:
10.3724/SP.J.1145.2016.09027
摘要:
精确识别活性微生物标识物是目前微生物油气勘探的技术核心,为深入认识各典型油气藏上方油气微生物标识物,采用高通量测序技术研究玉北地区油气富集带上方的油气微生物物种丰度、分布、群落结构等特征. 结果显示油气点上方样品较之背景非油气点具有更多的特有物种,其中油气点样品中含199种,背景非油气点样品中含77种. 从中共鉴定出10种主要油气微生物,发现其中的3类优势物种(假单胞菌属、芽孢杆菌属和土壤杆菌属)是利用烃类兼性生长的微生物;同时通过解析统计学差异值较高的生物标记物时,发现两种统计学差异值大于5的微生物,分别为背景非油气点上的弧菌科Vibrionaceae维氏气单胞菌和油气点上的方肠杆菌科Erythrobacteraceae赤杆菌. 本研究可为油气微生物勘探提供直接实验依据. (图8 参20)
Abstract:
Identification of active microbial markers is the core of microbial prospecting for oil and gas. However, current studies lack an in-depth understanding of oil and gas microbial markers above the typical reservoirs. In order to further understand the microbial information above the oil and gas fields, the microbial diversity in the soil above the Yubei oil field was studied. In this study, high-throughput sequencing techniques were used to investigate the abundance of oil and gas microbial species and community structure in the Yubei area. The results showed that samples obtained above the oil and gas spots contained more endemic species than those from obtained from the background non-oil and gas spots; 199 different microbes were present in the oil and gas samples compared with 77 different microbes in the non-oil and gas samples. Ten major oil-gas microbes were identified, and three dominant species (Pseudomonas, Bacillus, and Agrobacterium) are facultative microbial organisms. Furthermore, two potential families of microbial indicator bacteria (Vibrionaceae and Erythrobacteraceae) were identified. Based on the abnormal distribution of oil and gas microbes, an oil and gas enrichment zone and potential favorable drilling targets are inferred in the Yubei area. The results of oil and gas microbe in Yubei area with microbial distribution of the characteristics in this study laid the foundation, in order to further study indicator bacteria for underlying oil and gas.

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更新日期/Last Update: 2017-04-25