|本期目录/Table of Contents|

[1]姚阳春,刘永胜,刘明春.中华猕猴桃‘红阳’Alase家族基因的克隆及表达分析[J].应用与环境生物学报,2017,23(2):209-214.[doi:10.3724/SP.J.1145.2016.05015]
 YAO Yangchun,LIU Yongsheng,& LIU Mingchun**.Cloning and expression analysis of Alase family genes in kiwifruit (Actinidia chinensis)[J].Chinese Journal of Applied & Environmental Biology,2017,23(2):209-214.[doi:10.3724/SP.J.1145.2016.05015]
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中华猕猴桃‘红阳’Alase家族基因的克隆及表达分析()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
23卷
期数:
2017年第2期
页码:
209-214
栏目:
研究论文
出版日期:
2017-04-25

文章信息/Info

Title:
Cloning and expression analysis of Alase family genes in kiwifruit (Actinidia chinensis)
作者:
姚阳春刘永胜刘明春
1四川大学生命科学学院生物资源与生态环境教育部重点实验室,水力学与山区河流开发保护国家重点实验室 成都 610065 2合肥工业大学生物与食品工程学院 合肥 230009
Author(s):
YAO Yangchun1 LIU Yongsheng1 2 & LIU Mingchun1**
1Key Laboratory of Ministry of Education for Bio-resource and Eco-environment, College of Life Sciences, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu 610065, China 2School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China
关键词:
醛糖酸内酯酶抗坏血酸生物信息学分析基因表达分析
Keywords:
aldonolactonase ascorbic acid bioinformatics gene expression analysis
分类号:
Q78 : S663.401
DOI:
10.3724/SP.J.1145.2016.05015
摘要:
在高等植物中醛糖酸内酯酶(Alase)催化L-半乳糖酸(L-GalA)形成抗坏血酸(Ascorbic acid,AsA)的前体物质L-半乳糖-1,4-内酯(L-GaIL),是AsA合成分支途径D-半乳糖醛酸途径中的关键步骤. 基于最新的猕猴桃基因组数据库,分离并克隆到3个Alase家族基因,分别命名为Alase1、Alase2、Alase3,并从基因信息、蛋白质理化性质、二级结构及序列特征方面对其进行预测及分析. 结果表明,Alase为亲水性蛋白,二级结构主要以α-螺旋和不规则卷曲为主,3个Alase基因具有高度的相似性. 定量RT-PCR分析表明3个Alase家族基因在不同组织中均有表达,且在成熟的叶片中表达量最高. 同时在猕猴桃果实发育及成熟过程中,Alase家族基因在幼果中的表达水平较低,而在果实发育后期及果实成熟过程中表达量不断升高. 本研究可为Alase家族基因后续功能研究及猕猴桃中AsA生物合成和积累分子机制解析奠定基础. (图5 表2 参22)
Abstract:
In higher plants, aldonolactonase (Alase) is an important enzyme in the D-galacturonate pathway, which converts L-galactonate to L-galactono-1,4-lactone, the precursor of ascorbic acid (AsA). Based on the recently published kiwifruit genome sequence, specific primers were designed to isolate Alase family genes and the physical and chemical characteristics, secondary structures, and conserved motifs among Alase family members were further predicted and analyzed. Using quantitative RT-PCR, relative expression levels of Alase family genes in different tissues were revealed in kiwifruit. We identified three Alase family genes, named as Alase1, Alase2, and Alase3. Our results indicated that Alase proteins are hydrophilic and the secondary structures mainly consist of α-helices and irregular curls. Motif analysis showed that these Alase members are highly conserved in kiwifruit. Moreover, quantitative RT-PCR analysis revealed that transcripts of Alase genes are detectable in all tissues examined with higher expression observed in mature leaves. Moreover, the expression of all Alase genes increased from 30 days after pollination during fruit development and ripening in kiwifruit. Our results provide a foundation for the functional analysis of Alase family genes in AsA biosynthesis and accumulation in kiwifruit.

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更新日期/Last Update: 2017-04-25