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[1]林先贵,胡君利,戴珏,等.丛枝菌根真菌群落结构与多样性研究方法概述及实例比较[J].应用与环境生物学报,2017,23(2):343-350.[doi:10.3724/SP.J.1145.2016.04008]
　LIN Xiangui**,HU Junli,DAI Jue,et al.Overview and comparison of research methods for determining the community structure and diversity of arbuscular mycorrhizal fungi[J].Chinese Journal of Applied & Environmental Biology,2017,23(2):343-350.[doi:10.3724/SP.J.1145.2016.04008]
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丛枝菌根真菌群落结构与多样性研究方法概述及实例比较()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
23卷
期数:
2017年第2期
页码:
343-350
栏目:
研究论文
出版日期:
2017-04-25

文章信息/Info

Title:
Overview and comparison of research methods for determining the community structure and diversity of arbuscular mycorrhizal fungi
作者:
林先贵; 胡君利; 戴珏; 王发园; 冯有智
1中国科学院南京土壤研究所，土壤与农业可持续发展国家重点实验室 南京 210008 2上海第二工业大学环境与材料工程学院，上海电子废弃物资源化产学研合作开发中心 上海 201209 3青岛科技大学环境与安全工程学院 青岛 266045
Author(s):
LIN Xiangui1**;  HU Junli1;  DAI Jue1;  2;  WANG Fayuan1;  3 & FENG Youzhi1
1State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China 2Shanghai Cooperative Centre for WEEE Recycling, School of Environmental and Materials Engineering, Shanghai Polytechnic University, Shanghai 201209, China 3College of Environment and Safety Engineering, Qingdao University of Science and Technology, Qingdao 266045, China
关键词:
丛枝菌根真菌; 孢子; 形态鉴定; PCR-DGGE; 454高通量测序
Keywords:
arbuscular mycorrhizal fungi;  spore;  morphological identification;  PCR-DGGE;  454 pyrosequencing
分类号:
Q93-3 : S154.36
DOI:
10.3724/SP.J.1145.2016.04008
摘要:
开展丛枝菌根（Arbuscular mycorrhizal，AM）真菌的群落结构与多样性研究对促进菌物多样性的保护与发掘及生态系统的可持续发展均具有重要意义，而研究方法是实现和推进相关研究的重要基础. 本文首先简要回顾AM真菌的研究方法发展历程，介绍基于孢子形态鉴定的形态学方法和以聚合酶链式反应（Polymerase chain reaction，PCR）、变性梯度凝胶电泳（Denaturing gradient gel electrophoresis，DGGE）、454高通量测序为代表的分子生物学方法的检测原理和分析步骤. 然后，结合本实验室利用孢子计数与鉴定、定量PCR与PCR-DGGE以及454高通量基因测序等3套检测手段解析长期不同施肥影响土壤AM真菌群落结构和多样性的研究案例，比较分析不同方法所得AM真菌群落结构与多样性的研究结果. 综合来看，不同方法既有相互印证和互为补充的效果，又有不尽一致甚至互相矛盾的地方. 最后，着重就AM真菌高通量测序技术的升级及与生物标记物方法、同位素标记技术等的联用提出了研究展望. （图5 参35）
Abstract:
Investigating the community structure and diversity of arbuscular mycorrhizal (AM) fungi is important for accelerating the protection and application of mycodiversity, and for the sustainable development of ecosystems. Nevertheless, establishment of related research methods is the key to realizing and promoting such investigations. In this paper, we first provide a very brief historical survey on research methods of AM fungi, and introduce the principles and steps of both morphological and molecular biological methods, including spore morphological identification, polymerase chain reaction (PCR), denaturing gradient gel electrophoresis (DGGE), and 454 pyrosequencing. Then, the results related to the long-term fertilization effects on soil AM fungal community structure and diversity revealed by spore calculation and identification, real-time PCR and PCR-DGGE, and 454 pyrosequencing were integrated for method comparison. Altogether, different methods could not only complement each other or have effects of mutual corroboration, but also have in consistencies and contradictions. Finally, the future development of research methods for AM fungi are proposed, including an upgrade of the pyrosequencing technique, and its combined application with biomarker methods, isotope labeling techniques, and others.

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更新日期/Last Update: 2017-04-25