|本期目录/Table of Contents|

[1]李晓亮,张良,张培玉,等.北极海洋沉积物低温硝化细菌的筛选、多样性与生长特性[J].应用与环境生物学报,2017,23(2):294-300.[doi: 10.3724/SP.J.1145.2016.04004]
 LI Xiaoliang,ZHANG Liang,et al.Screening, biodiversity, and growth characteristics of cold-adapted nitrobacteria isolated from Arctic Ocean sediments[J].Chinese Journal of Applied & Environmental Biology,2017,23(2):294-300.[doi: 10.3724/SP.J.1145.2016.04004]
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北极海洋沉积物低温硝化细菌的筛选、多样性与生长特性()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
23卷
期数:
2017年第2期
页码:
294-300
栏目:
研究论文
出版日期:
2017-04-25

文章信息/Info

Title:
Screening, biodiversity, and growth characteristics of cold-adapted nitrobacteria isolated from Arctic Ocean sediments
作者:
李晓亮张良张培玉林学政
1青岛大学环境科学与工程学院 青岛 266071 2国家海洋局第一海洋研究所 青岛 266061
Author(s):
LI Xiaoliang1 2 ZHANG Liang2 ZHANG Peiyu1 & LIN Xuezheng2**
1College of Environmental Science and Engineering, Qingdao University, Qingdao 266071, China 2First Institute of Oceanography, State Oceanic Administration of China, Qingdao 266061, China
关键词:
北极海洋沉积物低温硝化细菌系统发育分析污水处理
Keywords:
Arctic Ocean sediments low temperature nitrobacteria phylogenetic analysis sewage treatment
分类号:
X172
DOI:
10.3724/SP.J.1145.2016.04004
摘要:
寒冷地区冬季低温污水的生物处理长期存在着处理效果差、难以达标排放等难题;而极地特殊的环境与气候造就了独特的极地微生物生态系统,是微生物新种质的资源宝库. 根据菌落形态特征,利用4种硝化细菌筛选培养基从12个站位的北极海洋沉积物中分离筛选,获得16S rRNA基因有效序列的低温硝化细菌达81株;基于16S rRNA基因的相似性分析与系统发育分析表明,分离到的菌株分属于细菌域的4个门、4个纲、8个目、16个科、22个属、30个种,其中γ-变形菌纲(γ-Proteobacteria)占大多数;有1株菌株与模式菌株的16S rRNA基因序列相似性小于97%,为潜在的新种. 大多数菌株的温度和耐NaCl浓度的生长范围分别为0-30 ℃和0-90‰,具有产蛋白酶、脲酶和β-半乳糖苷酶等胞外水解酶和利用葡萄糖、甘露醇和麦芽糖等多种碳源生长的能力. 本研究结果可为北方地区寒冷季节低温生活污水的生物处理提供新的菌种资源. (图2 表4 参24)
Abstract:
In cold areas, wastewater biotreatment techniques have some shortcomings such as low efficiency and difficulties in achieving emission standards because of the long cold winter and the low temperature of wastewater. The special environment and climate in polar regions has created a unique microbial ecosystem and species resources. On the basis of morphological characters, a total of 81 cold-adapted nitrobacteria were isolated from 12 Arctic Ocean sediments with four kinds of screening media. Phylogenetic analysis on the basis of 16S rRNA gene sequences showed that these isolates belonged to 4 phyla, 4 classes, 8 orders, 16 families, 22 genera, and 30 species. One isolate with an 16S rRNA gene sequence similarity less than 97% with the closest type strain might be a novel species. The growth temperature and NaCl tolerance range of most of the representative strains was 0–30 ℃ and 0–90‰, respectively. These isolates could produce many extracellular enzymes such as protease, urease, and β-galactosidase, and they could grow by utilizing many kinds of carbon sources, including glucose, mannitol, and maltose. This study can offer new microbial resources for improving sewage treatment at low temperature.

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更新日期/Last Update: 2017-04-25