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[1]刘炜婳,林玉玲,赖钟雄.福州宦溪野生蕉CBF7的cDNA与启动子克隆及在不同温度下的表达特性[J].应用与环境生物学报,2017,23(2):200-208.[doi:10.3724/SP.J.1145.2016.03032]
 LIU Weihua,LIN Yuling & LAI Zhongxiong**.Cloning and expression profile of the cDNA and promoter of CBF7 in wild banana (Musa itinerans) from Huanxi of Fuzhou City[J].Chinese Journal of Applied & Environmental Biology,2017,23(2):200-208.[doi:10.3724/SP.J.1145.2016.03032]
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福州宦溪野生蕉CBF7的cDNA与启动子克隆及在不同温度下的表达特性()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
23卷
期数:
2017年第2期
页码:
200-208
栏目:
研究论文
出版日期:
2017-04-25

文章信息/Info

Title:
Cloning and expression profile of the cDNA and promoter of CBF7 in wild banana (Musa itinerans) from Huanxi of Fuzhou City
作者:
刘炜婳林玉玲赖钟雄
福建农林大学园艺植物生物工程研究所 福州 350002
Author(s):
LIU Weihua LIN Yuling & LAI Zhongxiong**
Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
关键词:
宦溪野生蕉CRT/DRE-binding factorcDNA启动子qPCR
Keywords:
Musa itinerans Cheesman CRT/DRE-binding factor cDNA promoter qPCR
分类号:
S668.101
DOI:
10.3724/SP.J.1145.2016.03032
摘要:
CBF(CRT/DRE-binding factor)在植物低温响应及抗寒性的形成中起着重要的作用. 香蕉基因组中只包含有1个CBF7基因,以香蕉基因组序列为参考,采用同源克隆的方法,从福建宦溪野生蕉(Musa itinerans)叶片中克隆CBF7-1基因的cDNA全长序列及其启动子序列. 生物信息学分析显示宦溪野生蕉的CBF7-1具有植物CBF家族的典型AP2结构域,物种间序列特异性强;启动子富含多种类型的顺式作用元件,并且具有CpG岛. qPCR分析表明CBF7-1在28 ℃时表达量最高,随着温度降低表达量逐渐下降,但在0 ℃时反而又升高. 这表明宦溪野生蕉的CBF7-1可能与低温胁迫、耐热性均相关. (图6 表1 参61)
Abstract:
The CBF pathway plays an important leading role in all of the pathways involved in plant cold acclimation. In the banana (Musa accuminata) genome, there is only one CBF sequence identified, CBF7. Therefore, in this study, the cDNA and promoter of CBF7-1 were cloned by the homological cloning method according to the genome data of CBF7, and then bioinformatics analyses were conducted. The CBF7-1 expression levels of the Huanxi wild banana (M. itinerans) grown under different temperatures were detected by qPCR assays. CBF7-1 contained the typical AP2 domain of the CBF family, and the CBF sequence was species-specific; the promoter of CBF7-1 also contained various cis-elements as well as CpG islands. The qPCR assays indicated that the expression level of CBF7-1 was the highest at a higher ambient temperature (28 ℃), and decreased gradually as the temperatures dropped, but then increased at 0 ℃. CBF7-1 expression was closely related to cold and higher ambient temperature treatments, showing the probable diversity of CBF functions in the wild banana.

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更新日期/Last Update: 2017-04-25