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[1]武志华,丁一秀,任兴波,等.粘细菌的活性检测及与环境因子的相关性[J].应用与环境生物学报,2017,23(2):244-250.[doi:10.3724/SP.J.1145.2016.03024]
 WU Zhihua,DING Yixiu,REN Xingbo,et al.Determination of antibiotic activity of myxobacteria and their correlation with environmental factors[J].Chinese Journal of Applied & Environmental Biology,2017,23(2):244-250.[doi:10.3724/SP.J.1145.2016.03024]
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粘细菌的活性检测及与环境因子的相关性()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
23卷
期数:
2017年第2期
页码:
244-250
栏目:
研究论文
出版日期:
2017-04-25

文章信息/Info

Title:
Determination of antibiotic activity of myxobacteria and their correlation with environmental factors
作者:
武志华丁一秀任兴波赵璞钰苏力特刘惠荣
内蒙古农业大学生命科学学院 呼和浩特 010010
Author(s):
WU Zhihua DING Yixiu REN Xingbo ZHAO Puyu SU Lite & LIU Huirong**
College of Life Sciences, Inner Mongolia Agricultural University, Hohhot 010010, China
关键词:
粘细菌分离鉴定抗菌活性环境参数相关性
Keywords:
myxobacteria isolation identification antibiotic activity environmental parameter correlation
分类号:
Q938 + Q939.9
DOI:
10.3724/SP.J.1145.2016.03024
摘要:
为筛选具有抗菌活性的粘细菌,利用兔粪诱导、大肠杆菌划线诱导和滤纸诱导3种方法从内蒙古凉城地区采集的20种土样中分离粘细菌,结合形态观察、生理生化特征和分子鉴定方法确定这些菌株的分类地位,通过平板对峙法分析抗菌活性,并采用统计学方法分析其与环境因子的相关性. 共分离得到140株菌,包括39株纯粘细菌,经鉴定分别为黄色粘球菌(17株)、橙色粘球菌(13株)、变绿粘球菌(5株),还有4株只鉴定到粘球菌属(Myxococcus sp.). 抗菌结果显示:39株纯菌均表现出可以对一种甚至多种指示菌产生抗性,其中37株抑制大肠杆菌的生长,35株抑制马铃薯晚疫病菌的生长,26株抑制金黄色葡萄球菌的生长,20株抑制酿酒酵母菌的生长,19株抑制枯草芽孢杆菌的生长. 在分析环境因子与粘细菌分布情况的关系时发现,分离出的溶细菌类群的菌株数、溶纤维素类群的菌株数及总的菌株数与土壤pH和速效钾的含量呈正相关,与含水量呈负相关,与其它参数无明显相关性. 综上,内蒙古凉城地区粘细菌的优势菌群为粘球菌属,且绝大多数都有不同程度的抗菌活性;结果可为丰富内蒙古地区粘细菌资源及抗马铃薯晚疫病农药研发奠定基础. (图3 表6 参30)
Abstract:
The aim of this study was to evaluate the antibiotic activity of myxobacteria and analyze the relationship between environmental factors and the distribution of myxobacteria. Myxobacteria were isolated from 20 soil samples by the rabbit dung pellets-inducing method, Escherichia coli-inducing method, and filter paper-inducing method. The purified strains were identified by morphological observation, physiological and biochemical characteristics, and 16S rDNA sequence analysis. Then, the antibiotic activities of the strains were determined by the plate confrontation method. The correlation of the distribution of the strains with environmental factors was analyzed by statistical analysis. A total of 140 strains were isolated in this experiment and 39 of them were purified. The identification results showed that these strains belonged to the genus Myxococcus, including M. xanthus (17 strains), M. flavescens (13 strains), M. virescens (5 strains), and 4 unidentified strains. Antibiotic activity tests indicated that all of the purified myxobacteria strains exhibited one or more kinds of antibiotic activities. Thirty-seven strains showed activity against E. coli, 35 strains showed activity against Phytophthora infestans, 26 strains showed activity against Staphylococcus aureus, 20 strains showed activity against Saccharomyces cerevisiae, and 19 strains showed activity against Bacillus subtilis. Analysis of the relationship between environmental factors and the distribution of myxobacteria indicated that the number of bacteriolytic myxobacteria, the number of celluloytic myxobacteria, and even the total number of strains had positive correlations with pH value and available potassium, and had negative correlations with the water content; no significant correlations were detected with the other parameters. Myxococcus is the dominant genus in the area of Liangcheng in Inner Mongolia, China, and the vast majority of the myxobacterial strains isolated from this region show different levels of antibiotic activities. This work contributes to enriching the myxobacterial resources in Inner Mongolia and will lay a foundation for further research on myxobacteria for the discovery and development of new agricultural chemicals.

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更新日期/Last Update: 2017-04-25