1 Zhuge J, Fang HY, Wang ZX, Chen DZ, Jin HR, Gu HL. Glycerol production by a novel osmotolerant yeast Candida glycerinogenes [J]. Appl Microbiol Biot, 2001, 55 (6): 686-692 2 Pavlik P, Simon M, Schuster T, Ruis H. The glycerol kinase (GUT1) gene of Saccharomyces cerevisiae: cloning and characterization [J]. Curr Genet, 1993, 24 (1): 21-25 3 R?nnow B, Kielland-Brandt MC. GUT2, a gene for mitochondrial glycerol 3-phosphate dehydrogenase of Saccharomyces cerevisiae [J]. Yeast, 1993, 9 (10): 1121-1130 4 Jung JY, Kim TY, Ng CY, Oh MK. Characterization of GCY1 in Saccharomyces cerevisiae by metabolic profiling [J]. J Appl Microbiol, 2012, 113 (6): 1468-1478 5 Norbeck J, Blomberg A. Metabolic and regulatory changes associated with growth of Saccharomyces cerevisiae in 1. 4 M NaCl. Evidence for osmotic induction of glycerol dissimilation via the dihydroxyacetone pathway [J]. J Biol Chem, 1997, 272: 5544-5554 6 Gancedo C, Llobell A, Ribas JC, Luchi F. Isolation and characterization of mutants from Schyzosaccharomyces pombe defective in glycerol catabolism [J]. Eur J Biochem, 1986, 159 (1): 171-174 7 Matsuzawa T, Hara F, Tohda H, Uemura H, Takegawa K. Promotion of glycerol utilization using ethanol and 1-propanol in Schizosaccharomyces pombe [J]. Appl Microbiol Biotechnol, 2012, 95 (2): 441-449 8 Kitagawa T, Tokuhiro K, Sugiyama H, Kohda K, Isono N, Hisamatsu M, Takahashi H, Imaeda T. Construction of a β-glucosidase expression system using the multistress-tolerant yeast Issatchenkia orientalis [J]. Appl Microbiol Biotechnol, 2010, 87 (5): 1841-1853 9 Hao J, Zhuge B, Hong Z, Lu XY, Fang HY, Zhuge J. Role of CgHOG1 in stress responses and glycerol overproduction of Candida glycerinogenes [J]. Curr Microbiol, 2016, 73 (6): 1-7 10 Chen XZ, Fang HY, Rao ZM, Shen W, Zhuge B, Zhuge J, Wang ZX. An efficient genetic transformation method for glycerol producer Candida glycerinogenes [J]. Microbiol Res, 2008, 163 (5): 531-537 11 Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding [J]. Anal Biochem, 1976, 72 (1-2): 248-254 12 Matsuzawa T, Ohashi T, Hosomi A, Tanaka N, Tohda H, Takegawa K. The gld1+ gene encoding glycerol dehydrogenase is required for glycerol metabolism in Schizosaccharomyces pombe [J]. Appl Microbiol Biot, 2010, 87 (2): 715-727 13 Chen X, Fang H, Rao Z, Shen W, Zhuge B, Wang ZX, Zhuge J. Cloning and characterization of a NAD+-dependent glycerol-3-phosphate dehydrogenase gene from Candida glycerinogenes, an industrial glycerol producer [J]. FEMS Yeast Res, 2008, 8 (5): 725-734 14 Postma E, Verduyn C, Scheffers WA, Vandjken JP. Enzymic analysis of the crabtree effect in glucose-limited chemostat cultures of Saccharomyces cerevisiae [J]. Appl Environ Microb, 1989, 55 (2): 468-477 15 Dong X, Fan Y, Zhang H, Zhong Y, Yang Y, Miao J, Hua S. Inhibitory effects of ionic liquids on the lactic dehydrogenase activity [J]. Int J Biol Macromol, 2016, 86: 155-161 16 杜星星, 诸葛斌, 陆信曜, 郭新坤, 方慧英, 宗红, 宋健. budC缺失对克雷伯氏菌联产3-羟基丙酸和1,3-丙二醇的影响[J]. 应用与环境生物学报, 2014, 20 (3): 371-376 [Du XX, Zhuge B, Lu XY, Guo XK, Fang HY, Zong H, Song J. Effect of budC deficiency on coproduct 3-hydroxypropionic acid and 1,3-propandediol by Klebsiella pneumoniae [J]. Chin J Appl Environ Biol, 2014, 20 (3): 371-376] 17 Molin M, Norbeck J, Blomberg A. Dihydroxyacetone kinases in Saccharomyces cerevisiae are involved in detoxification of dihydroxyacetone [J]. J Biol Chem, 2003, 278 (3): 1415-1423 18 Ji H, Lu XY, Wang CY, Zong H, Fang HY, Sun J, Zhuge J, Zhuge B. Identification of a novel HOG1 homologue from an industrial glycerol producer Candida glycerinogenes [J]. Curr Microbiol, 2014, 69 (6): 909-914 19 Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2?ΔΔCTmethod [J]. Methods, 2001, 25 (4): 402-408 20 Molin M, Pilon M, Blomberg A. Dihydroxyacetone-induced death is accompanied by advanced glycation endproduct formation in selected proteins of Saccharomyces cerevisiae and Caenorhabditis elegans [J]. Proteomics, 2007, 7 (20): 3764-3774 21 Babel W, Hofmann KH. The relation between the assimilation of methanol and glycerol in yeasts [J]. Arch Microbiol, 1982, 132 (2): 179-184
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