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[1]宋宇迪,董玉秀,马新宇,等.降解植物甾醇为甾体9α-OH-AD菌株的筛选与鉴定[J].应用与环境生物学报,2017,23(06):1022-1027.[doi:10.3724/SP.J.1145.2017.01011]
 SONG Yudi,DONG Yuxiu,MA Xinyu,et al.Screening and identification of a strain degrading phytosterols to 9α-hydroxyandrost-4-ene-3,17-dione[J].Chinese Journal of Applied & Environmental Biology,2017,23(06):1022-1027.[doi:10.3724/SP.J.1145.2017.01011]
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降解植物甾醇为甾体9α-OH-AD菌株的筛选与鉴定()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
23卷
期数:
2017年06期
页码:
1022-1027
栏目:
微生物资源发掘与生物合成专栏论文
出版日期:
2017-12-25

文章信息/Info

Title:
Screening and identification of a strain degrading phytosterols to 9α-hydroxyandrost-4-ene-3,17-dione
作者:
宋宇迪 董玉秀 马新宇 张晓娟 陈志蔚 蔡兆培 李会 史劲松 许正宏
1江南大学药学院 无锡 214122 2大丰市佳丰油脂有限责任公司 盐城 224100 3江南大学粮食发酵工艺与技术国家工程实验室 无锡 214122
Author(s):
SONG Yudi1 DONG Yuxiu1 MA Xinyu1 ZHANG Xiaojuan1 CHEN Zhiwei2 CAI Zhaopei2 LI Hui1 2** SHI Jinsong1 & XU Zhenghong3
1 School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, China 2 Dafeng Jiafeng Oil Co., Ltd., Yancheng 224100, China 3 National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China
关键词:
植物甾醇分枝杆菌微生物转化9α-OH-AD吐温80
Keywords:
phytosterols Mycobacterium sp. microbial transformation 9α-OH-AD Tween-80
分类号:
Q936
DOI:
10.3724/SP.J.1145.2017.01011
摘要:
9α-羟基雄甾-4-烯-3,17-二酮(9α-OH-AD)是重要的甾体药物中间体,多用于生产糖皮质激素类药物. 通过微生物法转化植物甾醇生成9α-OH-AD,将为工业化生产提供极大便利. 从榨油厂、油菜花田等多处土壤中筛选出1株高效转化植物甾醇为9α-OH-AD的菌株,根据16S rDNA序列比对并结合菌株的形态特征、生理生化特征分析,鉴定其为分枝杆菌属,并将其命名为Mycobacterium sp. LY-1. 该菌在往复式摇床中培养,当培养条件为温度30 ℃、转速120 r /min、转化时间7 d、底物添加浓度为5 g/L时,9α-OH-AD产物得率达到16.2%. 为解决底物植物甾醇在水中溶解性较差的问题,考察了助溶剂(吐温80、β-环糊精)对植物甾醇转化产甾药中间体9α-OH-AD效率的影响. 经过筛选,最终确定最适助溶剂为0.1%的吐温80. 在此条件下,当底物投料浓度为15 g/L时,9α-OH-AD的浓度达到3.9 g/L,产物摩尔得率提高至35.1%. 本研究表明菌株LY-1转化效率好,可为后续的代谢工程改造提供便利并为后期用于工业化生产奠定基础. (图8 表2 参17)
Abstract:
9α-Hydroxyandrost-4-ene-3,17-dione (9α-OH-AD) is an important precursor in the manufacture of a number of modern glucocorticoid drugs. It may provide great convenience via the microbial conversion of phytosterols to 9α-OH-AD for industrial production. Here, a strain that can transform phytosterols to 9α-OH-AD was isolated from the soils of an oil mill, rape field, and other sources. Based on the sequence analysis of 16S rDNA and a series of morphological and biochemical characteristics, it was identified as Mycobacterium sp. and named as Mycobacterium sp. LY-1. Strain LY-1 was then cultivated in a rotary shaker at 30 ℃ and 120 r/min. After transformation for 7 days, the molar yield of 9α-OH-AD reached 16.2% with the addition of substrate at 5 g/L. To solve the low-solubility problem of the substrate phytosterols in water, the effects of a cosolvent (Tween 80 and β-cyclodextrin) on the biotransformation efficacy of phytosterols to drug intermediate 9α-OH-AD were investigated. Through the screening, the optimal feeding method was addition of 0.1% Tween 80. In this condition, the concentration of 9α-OH-AD was 3.9 g/L, and the molar yield of 9α-OH-AD reached 35.1% with the addition of substrate at 15 g/L. These results suggest that strain LY-1 has good transformation efficacy for a potential industrial application, and it laid the foundation for later industrial production.

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相似文献/References:

[1]杨亚力,杨顺楷,吴中柳.偶发分枝杆菌发酵断甾醇侧链积累9α-羟基雄烯二酮[J].应用与环境生物学报,2015,21(02):256.[doi:10.3724/SP.J.1145.2014.07022]
 YANG Yali,YANG Shunkai,WU Zhongliu.Development of 9α-hydroxy-androst-4-ene-3,17-dione (9α-OH-AD) through cleaving sterol sidechain by fermentation of Mycobacterium fortuitum[J].Chinese Journal of Applied & Environmental Biology,2015,21(06):256.[doi:10.3724/SP.J.1145.2014.07022]

更新日期/Last Update: 2017-12-25