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[1]李森森,马大龙,王璐璐,等.不同干扰方式下松江湿地沉积物细菌群落结构特征[J].应用与环境生物学报,2017,23(06):1074-1080.[doi:10.3724/SP.J.1145.2016.12008]
 LI Sensen,MA Dalong**,WANG Lulu,et al.Characteristics of bacterial communities in Songjiang wetland sediments subject to different disturbances[J].Chinese Journal of Applied & Environmental Biology,2017,23(06):1074-1080.[doi:10.3724/SP.J.1145.2016.12008]
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不同干扰方式下松江湿地沉积物细菌群落结构特征()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
23卷
期数:
2017年06期
页码:
1074-1080
栏目:
研究论文
出版日期:
2017-12-25

文章信息/Info

Title:
Characteristics of bacterial communities in Songjiang wetland sediments subject to different disturbances
作者:
李森森 马大龙 王璐璐 李莹 苑亚茹
哈尔滨师范大学地理科学学院 哈尔滨 150025
Author(s):
LI Sensen MA Dalong** WANG Lulu LI Ying & YUAN Yaru
College of Geographical Sciences, Harbin Normal University, Harbin 150025, China
关键词:
松江湿地T-RFLP细菌群落环境因子
Keywords:
Songjiang wetland T-RFLP bacterial community environmental factor
分类号:
X172
DOI:
10.3724/SP.J.1145.2016.12008
摘要:
松江湿地是我国面积最大的天然城市湿地,沉积物微生物对湿地环境变化响应敏感. 为了解不同干扰方式下松江湿地细菌群落多样性变化特征,运用末端限制性片段长度多态性(T-RFLP)技术,对比分析4种干扰方式(农业、工业、旅游和保护)对沉积物细菌群落结构的影响. 结果发现:片段长度为91.5 bp的T-RF普遍存在于6个湿地中,除呼兰河口(农业)湿地外,均为最优势菌;87.5 bp T-RF只存在于呼兰河口(农业)和阿什河(工业)湿地,且为呼兰河口(农业)湿地最优势菌(41.15%);93.5 bp T-RF出现在金河湾(旅游)、白鱼泡(旅游)、太阳岛(旅游)和滨江(保护)湿地. 细菌多样性指数滨江(保护)湿地最高,呼兰河口(农业)湿地最低. 序列比对表明这些优势菌群分别属于变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)和厚壁菌门(Firmicutes). 冗余分析(RDA)结果表明,呼兰河口湿地(农业)细菌群落受总氮(TN)影响较大;阿什河湿地(工业)细菌群落与总碳(TC)、总磷(TP)、TC/TN和pH具有较高的相关性;太阳岛湿地(旅游)受pH和TC/TN影响较大;而金河湾(旅游)、白鱼泡(旅游)和滨江(保护)湿地细菌群落与环境因子相关性较小. 本研究表明不同干扰方式下松江湿地沉积物细菌群落特征及其影响因子存在显著差异,结果可为松江湿地生态系统的保护与恢复提供科学依据. (图3 表3 参30)
Abstract:
Songjiang wetland is the largest natural, urban wetland in China, and its sedimentary microorganisms are sensitive to changes in the wetland environment. In order to investigate variations in bacterial community diversity in Songjiang wetland, the effect of four kinds of disturbances (agriculture, industry, tourism, and protection) on the sedimentary bacterial community structure was analyzed using a terminal restriction fragment length polymorphism (T-RFLP) technique. The study found that the 91.5 bp T-RF generally existed in six wetlands and was the predominant bacteria, except in the Hulan Estuary (agriculture) wetland. The 87.5 bp T-RF was only found in the Hulan Estuary (agriculture) and Ashi River (industry) wetlands, and it was the predominant bacteria (41.15%) in the Hulan Estuary (agriculture) wetland. The 93.5 bp T-RF appeared in the Jinhe Bay (tourism), Baiyupao (tourism), Sun Island (tourism), and Binjiang (protection) wetlands. The above results show that there were significant variations in the bacterial community structure in wetlands subject to different disturbances. The highest bacterial diversity index appeared in Binjiang (protection) wetland, whereas the lowest emerged in the Hulan Estuary (agriculture) wetland. Sequence alignment revealed that the dominant bacteria belonged to Proteobacteria, Bacteroidetes, and Firmicutes. The results of a redundancy analysis (RDA) showed that the bacterial community in Hulan Estuary (agriculture) wetland was drastically affected by total nitrogen (TN); the bacterial community in Ashi River (industry) wetland had a high correlation with total phosphorus (TP), total carbon (TC), TC/TN, and pH; and the bacterial community in Sun Island (tourism) wetland was heavily influenced by pH and TC/TN. The bacterial communities in Jinhe Bay (tourism), Baiyupao (tourism), and Binjiang (protection) wetlands were less affected by environmental factors. The results show that there were significant variations in the characteristics of sedimentary bacterial communities and their influencing factors in Songjiang wetlands subject to different disturbances, which could provide a scientific basis for the protection and restoration of wetland ecosystems in Songjiang.

参考文献/References:

王晓龙, 徐立刚, 姚鑫, 白丽, 张奇. 鄱阳湖典型湿地植物群落土壤微生物量特征[J]. 生态学报, 2010, 30 (18): 5033-5042 [Wang XL, Xu LG, Yao X, Bai L, Zhang Q. Analysis on the soil microbial biomass in typical hygrophilous vegetation of Poyang Lake [J]. Acta Ecol Sin, 2010, 30 (18): 5033-5042]
裴希超, 许艳丽, 魏巍. 湿地生态系统土壤微生物研究进展[J]. 湿地科学, 2009, 7 (2): 181-186 [Pei XC, Xu YL, Wei W. A review on soil microorganisms in wetland ecosystem [J]. Wetland Sci, 2009, 7 (2): 181-186]
Mitchell PJ, Simpson AJ, Soong R, Simpson MJ. Shifts in microbial community and water-extractable organic matter composition with biochar amendment in a temperate forest soil [J]. Soil Biol Biochem, 2014, 81: 244-254
贺纪正, 王军涛. 土壤微生物群落构建理论与时空演变特征[J]. 生态学报, 2015, 35 (20): 6575-6583 [He JZ, Wang JT. Mechanisms of community organization and spatiotemporal patterns of soil microbial communities [J]. Acta Ecol Sin, 2015, 35 (20): 6575-6583]
袁三青, 薛燕芬, 高鹏, 汪卫东, 马延和, 李希明, 许国旺. T-RFLP技术分析油藏微生物多样性[J]. 微生物学报, 2007, 47 (2): 290-294 [Yuan SQ, Xue YF, Gao P, Wang WD, Ma YH, Li XM, Xu GW. Microbial diversity in Shengli petroleum reservoirs analyzed by T-RFLP [J]. Acta Microbiol Sin, 2007, 47 (2): 290-294]
Wu HP, Zeng GM, Liang J, Guo SL, Dai J, Lu LH, Wei Z, Xu P, Li F, Yuan YJ, He XX. Effect of early dry season induced by the Three Gorges Dam on the soil microbial biomass and bacterial community structure in the Dongting Lake wetland [J]. Ecol Indicators, 2015, 53: 129-136
罗剑飞, 林炜铁, 任杰, 崔华平. T-RFLP技术及其在硝化细菌群落分析中的应用[J]. 微生物学通报, 2008, 35 (3): 456-461 [Luo JF, Lin WT, Ren J, Cui HP. T-RFLP technique and its application on community analysis of nitrifying bacteria [J]. Microbiology, 2008, 35 (3): 456-461]
Thomson BC, Tisserant E, Plassart P, Uroz S, Griffiths RI, Hannula SE, Buee M, Mougel C, Ranjard L, Van Veen JA, Martin F, Bailey MJ, Lemanceau P. Soil conditions and land use intensification effects on soil microbial communities across a range of European field sites [J]. Soil Biol Biochem, 2015, 88: 403-413
唐婧, 徐小蓉, 商传禹, 牛晓娟, 张习敏, 乙引. 南明河城区河段细菌多样性与环境因子的关系[J]. 微生物学报, 2015, 55 (8): 1050-1059 [Tang J, Xu XR, Shang CY, Niu XJ, Zhang XM, Yi Y. Association of bacterial diversity in city area of Nanming river with environmental factors [J]. Acta Microbiol Sin, 2015, 55 (8): 1050-1059]
刘兰英, 吕新, 李巍, 陈丽华, 李玥仁, 林碧娇. 福州左海湖细菌群落与环境的关系[J]. 微生物学报, 2015, 55 (9): 1177-1189 [Liu LY, Lü X, Li W, Chen LH, Li YR, Lin BJ. Relationship between bacterial community and its environmental in Zuohai Lake, China [J]. Acta Microbiol Sin, 2015, 55 (9): 1177-1189]
宋洪宁, 杜秉海, 张明岩, 路晓萌, 李正华, 丁延芹. 环境因素对东平湖沉积物细菌群落结构的影响[J]. 微生物学报, 2010, 50 (8): 1065-1071 [Song HN, Du BH, Zhang MY, Lu XM, Li ZH, Ding YQ. Effect of environmental factors on bacterial community in Lake Dongping sediment [J]. Acta Microbiol Sin, 2010, 50 (8): 1065-1071]
杜萍, 刘晶晶, 曾江宁, 陈全震, 朱旭宇, 高瑜. 西门岛红树林沉积物细菌分布与环境特征[J]. 海洋环境科学, 2014, 33 (5): 763-771 [Du P, Liu JJ, Zeng JN, Chen QZ, Zhu XY, Gao Y. Bacteria distribution and environmental characteristics in the sediment of Ximen Island mangrove [J]. Mar Environ Sci, 2014, 33 (5): 763-771]
蒋本超, 戚秀云. 哈尔滨市松江湿地保护现状及生态修复策略研究[J]. 环境科学与管理, 2016, 41 (2): 146-149 [Jiang BC, Qi XY. Protection status and ecological restoration strategy of Songhua River Wetland in Harbin City [J]. Environ Sci Manage, 2016, 41 (2): 146-149]
于少鹏, 孙雅萍, 谷晓红, 蔡春苗, 王孝本, 沙迪. 哈尔滨天然湿地生态环境可持续发展研究[J]. 国土与自然资源研究, 2008 (2): 67-68 [Yu SP, Sun YP, Gu XH, Cai CM, Wang XB, Sha D. A study on the sustainable development of eco-environment of natural wetland in Harbin [J]. Territory Nat Res Study, 2008 (2): 67-68]
赵志强, 吴妍. 哈尔滨松江湿地风景资源评价[J]. 国土与自然资源研究, 2011 (4): 62-63 [Zhao ZQ, Wu Y. Evaluation on the scenery resources of Harbin Songjiang wetland [J]. Terr Nat Res Study, 2011 (4)4: 62-63]
鲍士旦. 土壤农化分析[M]. 3版. 北京: 中国农业出版社, 2005 [Bao SD. Soil and agricultural chemistry analysis [M]. 3rd ed. Beijing: China Agriculture Press, 2005]
冯广达, 陈美标, 羊宋贞, 朱红惠. 用于PCR扩增的细菌DNA提取方法比较[J]. 华南农业大学学报, 2013, 34 (3): 439-442 [Feng GD, Chen MB, Yang SZ, Zhu HH. A comparative study on bacteria DNA extraction methods used for PCR amplification [J]. J South China Agric Univ, 2013, 34 (3): 439-442]
Hinsa-Leasure SM, Bhavaraju L, Rodrigues JLM, Bakermans C, Gilichinsky DA, Tiedje JM. Characterization of a bacterial community from a Northeast Siberian seacoast permafrost sample [J]. FEMS Microbiol Ecol, 2010, 74 (1): 103-113
徐爱玲, 吴等等, 宋志文, 任杰, 夏岩, 董珊珊, 刘梦. 引物选择对污泥微生物多样性分析的影响[J]. 环境科学, 2013, 34 (9): 3620-3626 [Xu AL, Wu DD, Song ZW, Ren J, Xia Y, Dong SS, Liu M. Effect of different primers on microbial community of activated sludge [J]. Environ Sci, 2013, 34 (9): 3620-3626]
郑景生, 吕蓓. PCR技术及实用方法[J]. 分子植物育种, 2003, 1 (3): 381-394 [Zheng JS, Lü B. PCR technique and its practical methods [J]. Mol Plant Breed, 2003, 1 (3): 381-394]
余素林, 吴晓磊, 钱易. 环境微生物群落分析的T-RFLP技术及其优化措施[J]. 应用与环境生物学报, 2006, 12 (6): 861-868 [Yu SL, Wu XL, Qian Y. Application and optmization of T-RFLP method for microbial community analysis [J]. Chin J Appl Environ Biol, 2006, 12 (6): 861-868]
叶华香, 臧淑英, 张丽娟, 张玉红. 扎龙湿地沉积物重金属空间分布特征及其潜在生态风险评价[J]. 环境科学, 2013, 34 (4): 1333-1339 [Ye HX, Zang SY, Zhang LJ, Zhang YH. Distribution and potential ecological risk assessment of heavy metals in sediments of Zhalong Wetland [J]. Environ Sci, 2013, 34 (4): 1333-1339]
Von Gunten HR, Sturm M, Moser RN. 200-year record of metals in lake sediments and natural background concentrations [J]. Environ Sci Technol, 1997, 31 (8): 2193-2197
Ma WK, Farrell RE, Siciliano SD. Nitrous oxide emissions from ephemeral wetland soils are correlated with microbial community composition [J]. Front Microbiol, 2011, 2 (1): 111-117
Stoeva MK, Aris-Brosou S, Chetelat J, Hintelmann H, Pelletier P, Poulain AJ. Microbial community structure in lake and wetland sediments from a high Arctic polar desert revealed by targeted transcriptomics [J]. PLoS ONE, 2014, 9 (3): 1-12
寇文伯, 黄正云, 张杰, 刘倩纯, 刘芳鹏, 刘以珍, 吴兰. 鄱阳湖湖泊细菌群落组成及结构——以松门山为例[J]. 生态学报, 2015, 35 (23): 7608-7614 [Kou WB, Huang ZY, Zhang J, Liu QC, Liu FP, Liu YZ, Wu L. Bacterial community structure and composition in Lake Poyang: a case study in the Songmenshan Region, China [J]. Acta Ecol Sin, 2015, 35 (23): 7608-7614]
蒋然, 王健鑫, 黄备, 张潘, 郑俊威, 俞凯成, 刘明华. 椒江口沉积物中细菌多样性初步研究[J]. 海洋与湖沼, 2015, 46 (4): 887-900 [Jiang R, Wang JX, Huang B, Zhang P, Zheng JW, Yu KC, Liu MH. Bacterial diversity insediments in Jiaojiang River Estuary [J]. Oceanol Limnol Sin, 2015, 46 (4): 887-900]
Peralta RM, Ahn C, Gillevet PM. Characterization of soil bacterial community structure and physicochemical properties in created and natural wetlands [J]. Sci Total Environ, 2013, 443 (3): 725-732
李磊, 曾薇, 张悦, 杨莹莹. 分子生物技术在污水处理系统内硝化菌群研究中的应用[J]. 应用与环境生物学报, 2010, 16 (1): 135-142 [Li L, Zeng W, Zhang Y, Yang YY. Application of molecular biological techniques for analyzing nitrifier communities in wastewater treatment [J]. Chin J Appl Environ Biol, 2010, 16 (1): 135-142]
张矛宇, 李强, 张杰, 侯若彤, 杨志荣, 罗璠. 中国新疆火焰山土壤环境及细菌群落结构[J]. 应用与环境生物学报, 2015, 21 (1): 155-163 [Zhang MY, Li Q, Zhang J, Hou RT, Yang ZR, Luo P. Soil fertility status and bacterial community structure on Huoyan Mountain, China [J]. Chin J Appl Environ Biol, 2015, 21 (1): 155-163]

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更新日期/Last Update: 2017-12-25