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[1]郭梦圆,高艳华,郑雨璋,等.冬虫夏草内生菌NS2-B1发酵培养基筛选[J].应用与环境生物学报,2017,23(05):939-944.[doi:10.3724/SP.J.1145.2016.11013]
　GUO Mengyuan,GAO Yanhua,ZHENG Yuzhang,et al.Screening of liquid fermentation media of an endophytic fungus NS2-B1 isolated from Ophiocordyceps sinensis[J].Chinese Journal of Applied & Environmental Biology,2017,23(05):939-944.[doi:10.3724/SP.J.1145.2016.11013]
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冬虫夏草内生菌NS2-B1发酵培养基筛选()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
23卷
期数:
2017年05期
页码:
939-944
栏目:
生物活性化合物的发现与开发专栏
出版日期:
2017-10-25

文章信息/Info

Title:
Screening of liquid fermentation media of an endophytic fungus NS2-B1 isolated from Ophiocordyceps sinensis
作者:
郭梦圆; 高艳华; 郑雨璋; 王萍; 夏飞; 周选围
1上海交通大学农业与生物学院植物生物技术研究中心，农业部都市农业（南方）重点实验室 上海 200240 2陕西科技大学食品与生物工程学院 西安 710021 3天津理工大学化学化工学院 天津 300384
Author(s):
GUO Mengyuan1;  GAO Yanhua1;  ZHENG Yuzhang1;  WANG Ping3;  XIA Fei2** & ZHOU Xuanwei1**
1Ministry of Agriculture Key Laboratory of Urban Agriculture (South), Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China 2 School of Food and Biological Engineering, Shaanxi University of Science & Technology, Xi’An 710021, China 3 College of Chemistry and Chemical Engineering, Tianjin University of Technology, Tianjin, 300384, China
关键词:
冬虫夏草; 内生真菌; 液体培养基; 正交试验; 代谢产物
Keywords:
Ophiocordyceps sinensis;  endophytic fungus;  liquid media;  orthogonal test;  metabolites
分类号:
Q93-3:Q929
DOI:
10.3724/SP.J.1145.2016.11013
摘要:
以核苷类物质为目标产物筛选冬虫夏草内生真菌NS2-B1生产具有和冬虫夏草相似活性成分的培养基. 通过单因素实验，分别从5种碳源（蔗糖、果糖、葡萄糖、麦芽糖、淀粉）和5种氮源（尿素、硝酸铵、牛肉膏、酵母膏、蛋白胨）中各筛选出2种最佳碳源和氮源；然后通过4因素3水平正交实验L9(34)，以代谢产物总核苷（尿苷、鸟苷、腺苷和虫草素）为指标筛选最佳的碳、氮源组合. 结果显示：NS2-B1液体发酵培养基组合为麦芽糖2%、淀粉1.5%、酵母膏1.5%、蛋白胨1%，附加KH2PO4 0.8 g/L和MgSO4.7H2O 0.8 g/L；利用该培养基在25 ℃、210 r/min条件下震荡培养，5 d后菌丝体发酵液中总核苷的产量达到（92.62 ± 4.19）μg/mL. 综上，从天然冬虫夏草中分离的内生真菌NS2-B1具有替代冬虫夏草生产核苷类物质的潜力. （图4 表4 参30）
Abstract:
Based on nucleosides as the target produces, the suitable liquid media for fermenting an endophytic fungus NS2-B1 isolated from Ophiocordyceps sinensis was investigated to produce active ingredients which were similar to O. sinensis. Two optimum carbon sources and two optimum nitrogen sources were selected from five carbon sources (including sucrose, fructose, glucose, maltose and starch) and five nitrogen sources (including urea, beef extract, yeast extractand peptone) by one-factor-at-a-time experiments. The suitable culture medium for producing the nucleosides was studied by orthogonal test. Table L9(34) was used to test four factors with three levels. The nucleosides (uridine, guanosine, adenosine, cordycepin) of O. sinensis metabolites in the mycelial fermentation broth were determined by HPLC. The results showed that the suitable liquid medium for the endophytic fungus isolated from O. sinensis consisted of 2% maltose, 1.5% starch, 1.5% yeast extract, 1% peptone, plus to 0.8 g/L KH2PO4 and 0.8 g/L MgSO4.7H2O. The yield of the nucleosides in the mycelial fermentation broth reached to 92.62 ± 4.19 μg/mL under the conditions of 25 ℃ and 210 r/min for 5 days. Therefore, the endophytic fungus NS2-B1 had the potential to replace the Ophiocordyceps sinensis in producing nucleosides.

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更新日期/Last Update: 2017-10-25