|本期目录/Table of Contents|

[1]陈旭,曾友竞,王嘉毅,等.龙眼miR159家族成员进化特性及时空表达[J].应用与环境生物学报,2017,23(04):602-608.[doi:10.3724/SP.J.1145.2017.03011]
　CHEN Xu,ZENG Youjing,WANG Jiayi,et al.Effect of main grain components on the starch swelling power of Tibetan hull-less barley (Hordeum vulgare var. nudum)[J].Chinese Journal of Applied & Environmental Biology,2017,23(04):602-608.[doi:10.3724/SP.J.1145.2017.03011]
点击复制

龙眼miR159家族成员进化特性及时空表达 ()
分享到：

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
23卷
期数:
2017年04期
页码:
602-608
栏目:
研究论文
出版日期:
2017-08-25

文章信息/Info

Title:
Effect of main grain components on the starch swelling power of Tibetan hull-less barley (Hordeum vulgare var. nudum)
作者:
陈旭; 曾友竞; 王嘉毅; 陈晓慧; 徐小萍; 李汉生; 张梓浩; 陈裕坤; 赖钟雄; 王天池; 林玉玲
福建农林大学园艺植物生物工程研究所 福州 350002
Author(s):
CHEN Xu;  ZENG Youjing;  WANG Jiayi;  CHEN Xiaohui;  XU Xiaoping;  LI Hansheng;  ZHANG Zihao;  CHEN Yukun;  LAI Zhongxiong;  WANG Tianchi** & LIN Yuling**
Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
关键词:
龙眼; miR159家族; 进化特性; 实时荧光定量
Keywords:
Dimocarpus longan;  miR159 family;  evolutionary characteristic;  real-time quantitative PCR
分类号:
S667.203
DOI:
10.3724/SP.J.1145.2017.03011
摘要:
为了解植物miR159家族成员的进化特性及其在不同组织部位的表达规律，以龙眼miR159家族为例对miRNA家族进行探讨. 通过龙眼miR159家族前体与成熟体序列比对、前体二级结构预测、系统发育进化树的构建、靶基因预测及实时荧光定量等手段，对龙眼miR159基因家族进行研究. 结果显示：龙眼miR159家族存在7个前体成员和6个成熟体成员，序列比对发现5个成熟体可以高度定位于7个前体序列中一个共有的、约20个碱基的保守区域，推测miR159成熟体主要来源于该区域；进一步分析发现6个成熟体序列中仅2个可定位于pre-MIR159中. mfold预测结果显示miR159家族7个前体的最小折叠自由能在-56.54 - -92.53 kal/mol之间，均能自发形成典型、稳定的茎环二级结构. 系统发育进化树显示龙眼miR159家族前体与成熟体成员均具有保守性与多样性. 靶基因预测发现龙眼miR159家族所有成员共预测出14个不同靶标，其中3个成员也可以作用于同一靶标. 实时荧光定量PCR显示，龙眼pre-MIR159家族成员间在不同组织部位表达量差异显著，但表达趋势却几乎一致，其中花器官中表达量最高，提示了dlo-miR159在生殖器官形成过程中具有重要的作用，尤其是在雄花中作用显著. 本研究表明龙眼miR159家族在进化过程中形成了保守性与多样性并存的进化特性，并且可能在花器官形成过程中发挥了重要的作用. （图5 表3 参36）
Abstract:
In order to understand the evolutionary characteristics of miR159 family members and their expression patterns in different tissues, the miR159 family of Dimocarpus longan was used as an example in this study to explore its evolutionary characteristics. The evolutionary characteristics of miR159 gene family and its expression in different tissues of D. longan were studied by comparing the precursor and mature miRNA sequences, predicting their secondary structure, constructing a phylogenetic tree, target genes, and detecting their expression levels by real-time fluorescence quantification. The results showed that the D. longan miR159 family contained six mature miRNA sequences and seven precursor sequences; they shared a conserved region of about 20 bases, suggesting that mature miR159 mainly originated from the conserved region of the precursor sequences. Among of the six mature miRNA sequences, only two could be perfectly located in pre-MIR159s. The mfold prediction showed that the minimum free radical energy of the 7 precursors of miR159 family was between -56.54 kal/mol and -92.53 kal/mol; the pre-MIR159s could form a typical and stable stem-loop secondary structure. Phylogenetic tree showed that the precursors of the D. longan miR159 family have some conservatism and diversity. Target gene prediction results showed that D. longan miR159 family has 14 different targets, and multiple members can target the same genes. Real-time quantitative PCR showed that the expression of pre-MIR159 members was different in different tissues, which was higher in floral organs, but the expression trend of each member was consistent, suggesting that D. longan miR159 family was important in the process of gametogenesis, especially in male flowers. The evolutionary characteristics of D. longan miR159 family were conservative and diverse, and they may have an important role in the process of floral organ formation.

参考文献/References:

1 Lee RC, Feinbaum RL, Ambros V. The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14 [J]. Cell, 1993, 75 (5): 843-854
2 Reinhart BJ, Slack FJ, Basson M, Pasquinelli AE, Bettinger JC, Rougvie AE, Ruvkun G. The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans [J]. Nature, 2000, 403 (6772): 901-906
3 张露. microRNAs在秀丽隐杆线虫逃避行为中的作用[D]. 昆明: 云南大学, 2015 [Zhang L. The role of microRNAs in avoidance behavior of Caenorhabditis elegans [D]. Kunming: Yunnan University, 2015]
4 Krol J, Loedige I, Filipowicz W. The widespread regulation of microRNA biogenesis, function and decay [J]. Nature Rev Gen, 2010, 11 (9): 597-610
5 王亚婷. 龙眼胚性愈伤组织中若干microRNA的初级体克隆及其功能分析[D]. 福州: 福建农林大学, 2013 [Wang YT. Studies on cloning of the primary transcripts and function analyses of some microRNAs from embryonic callus in Dimocarpus longan Lour. [D]. Fuzhou: Fujian Agriculture and Forestry University, 2013]
6 Kidner CA, Martienssen RA. The developmental role of microRNA in plants [J]. Curr Opin Plant Biol, 2005, 8 (1): 38-44
7 郑聿贤. miR159的克隆及抗旱性研究[D]. 杨陵: 西北农林科技大学, 2014 [Zheng YX. Cloning and research on the drought resistance of miR159 [D]. Yangling: Northwest A&F University, 2014]
8 Harvey JL, Evans HC. Aquatic Plant Control Research Program. Assessment of fungal pathogens as biocontrol agents of Myriophyllum Spicatum [R]. United Kingdom: International Institute Biological Control Ascot, 1997
9 Millar AA, Gubler F. The arabidopsis GAMYB-like genes, MYB33 and MYB65, are microRNA-regulated genes that redundantly facilitate anther development [J]. Plant Cell, 2005, 17 (3): 705-721
10 李晓燕. miR159和miR172表达对大岩桐花发育的调控作用及其机理研究[D]. 杭州: 浙江大学, 2012 [Li XY. Function and regulatory mechanism of miR159 and miR172 in floral development in gloxinia (Sinningia Speciosa) [D]. Hangzhou: Zhejiang University, 2012]
11 张晓玲, 龙芸, 葛飞, 管中荣, 张晓祥, 王艳丽, 潘光堂. 玉米幼胚胚性愈伤组织再生能力的相关研究[J]. 遗传, 2017, 39 (2): 143-155 [Zhang XL, Long Y, Ge F, Guan ZR, Zhang XX, Wang YL, Pan GT. A genetic study of the regeneration capacity of embryonic callus from the maize immature embryo culture [J]. Hereditas, 2017, 39 (2): 143-155]
12 刘伟娜. 厚荚相思器官发生途径microRNA及其靶基因的鉴定与表达研究[D]. 北京: 北京林业大学, 2015 [Liu WN. Identification and expression of MicroRNAs and their targets in Acacia Crassicarpa Organogenesis [D]. Beijing: Beijing Forestry University, 2015]
13 Lai ZX, Lin YL. Analysis of the global transcriptome of longan (Dimocarpus longan Lour.) embryogenic callus using Illumina paired-end sequencing [J]. BMC Genom, 2013, 14 (1): 561
14 Lin YL, Lai ZX. Comparative analysis reveals dynamic changes in miRNAs and their targets and expression during somatic embryogenesis in longan (Dimocarpus longan Lour.) [J]. PLoS ONE, 2013, 8 (4): e60337
15 Lin YL, Lai ZX, Lin L. Endogenous target mimics, microRNA167, and its targets ARF6 and ARF8 during somatic embryo development in Dimocarpus longan Lour. [J]. Mol Breed, 2015, 35 (12): 227
16 赖瑞联, 林玉玲, 赖钟雄. 龙眼生长素受体基因TIR1的克隆及其与miR393互作关系[J]. 应用与环境生物学报, 2016, 22 (1): 95-102 [Lai RL, Lin YL, Lai ZX. Cloning of auxin receptor gene TIR1 and its interaction with miR393 in Dimocarpus longan Lour. [J]. Chin J Appl Environ Biol, 2016, 22 (1): 95-102]
17 Lin YL, Lai ZX. Evaluation of suitable reference genes for normalization of microRNA expression by real-time reverse transcription PCR analysis during longan somatic embryogenesis [J]. Plant Physiol Biochem, 2013, 66: 20-25
18 胡玉婷. 植物活性成分与蛋白质的结合性质及对蛋白质结构的影响[D]. 南昌: 南昌大学, 2014 [Hu YT. The binding properties of the plant active ingredients with serum albumins and the effects on the structure of proteins [D]. Nanchang: Nanchang University, 2014]
19 Reinhart BJ, Weinstein EG, Rhoades MW, Bartel B, Bartel DP. MicroRNAs in plants [J]. Genes Dev, 2002, 16 (13): 1616-1626
20 Llave C, Xie Z, Kasschau KD, Carrington JC. Cleavage of Scarecrow-like mRNA targets directed by a class of Arabidopsis miRNA [J]. Science, 2002, 297 (5589): 2053-2056
21 Rhoades MW, Reinhart BJ, Lim LP, Burge CB, Bartel B, Bartel DP. Prediction of plant microRNA targets [J]. Cell, 2002, 110 (4): 513-520
22 Palatnik JF, Allen E, Wu X, Schommer C, Schwab R, Carrington JC, Weigel D. Control of leaf morphogenesis by microRNAs [J]. Nature, 2003, 425 (6955): 257-263
23 Dezulian T, Palatnik JF, Huson D, Weigel D. Conservation and divergence of microRNA families in plants [J]. Gen Biol, 2005, 6: 13
24 Patade VY, Suprasanna P. Short-term salt and PEG stresses regulate expression of microRNA, miR159 in sugarcane leaves [J]. J Crop Sci Biotechnol, 2010, 13 (3): 177-182
25 李伟, 韩蕾, 钱永强, 孙振元. 植物NAC转录因子的种类, 特征及功能[J]. 应用与环境生物学报, 2011, 17 (4): 596-606 [Li W, Han L, Qian YQ, Sun ZY. Characteristics and functions of NAC transcription factors in plants [J]. Chin J Appl Environ Biol, 2011, 17 (4): 596-606]
26 魏强, 梁永宏, 李广林. 植物miRNA的进化[J]. 遗传, 2013, 35 (3): 315-323 [Wei Q, Liang YH, Li GL. Evolution of miRNA in plants [J]. Hereditas, 2013, 35 (3): 315-323]
27 Jiang D, Yin C, Yu A, Zhou X, Liang W, Yuan Z, Zhang D. Duplication and expression analysis of multicopy miRNA gene family members in Arabidopsis and rice [J]. Cell Res, 2006, 16 (5): 507-518
28 Yang T, Xue L, An L. Functional diversity of miRNA in plants [J]. Plant Sci, 2007, 172 (3): 423-432
29 Ellis J, Dodds P, Pryor T. Structure, function and evolution of plant disease resistance genes [J]. Curr Opin Plant Biol, 2000, 3 (4): 278-284
30 侍婷, 高志红, 章镇, 庄维兵. MicroRNA参与植物花发育调控的研究进展[J]. 中国农学通报, 2010, 26 (13): 267-271 [Shi T, Gao ZH, Zhang Z, Zhuang WB. Advance of research on MicroRNA in flower development regulation [J]. Chin Agric Sci Bull, 2010, 26 (13): 267-271]
31 王波, 冯晓黎, 张富春. 植物中microRNA的合成及在发育和抗逆中的作用[J]. 植物生理学通讯, 2006, 42 (3): 581-588 [Wang B, Feng XL, Zhang FC. The synthesis of MicroRNAs and its role in development and stress resistance of plants [J]. Plant Physiol Commun, 2006, 42 (3): 581-588]
32 高阳, 赵琳, 李文滨. 植物赤霉素信号转导途径中GAMYB基因及其在大豆中的研究[J]. 大豆科学, 2010, 29 (4): 717-720 [Gao Y, Zhao L, Li WB. Advances on the study of GAMYB gene in gibberellin signal transduction in soybean and other plant [J]. Soyb Sci, 2010, 29 (4): 717-720 ]
33 Gocal GFW, Sheldon CC, Gubler F, Moritz T, Bagnall DJ, MacMillan CP, King R W. GAMYB-like genes, flowering, and gibberellin signaling in Arabidopsis [J]. Plant Physiol, 2001, 127 (4): 1682-1693
34 Csukasi F, Donaire L, Casa?al A, Martínez-Priego L, Botella MA, Medina-Escobar N, Valpuesta V. Two strawberry miR159 family members display developmental-specific expression patterns in the fruit receptacle and cooperatively regulate Fa-GAMYB [J]. New Phytol, 2012, 195 (1): 47-57
35 张微, 李锡香. 黄瓜花芽性别分化的研究进展与展望[J]. 华北农学报, 2015, 30: 74-80 [Zhang W, Li XX. The research advances on differentiation of cucumber flower bud [J]. Acta Agric Bor Sin, 2015, 30: 74-80]
36 Li WF, Zhang SG, Han SY, Wu T, Zhang JH, Qi LW. Regulation of LaMYB33 by miR159 during maintenance of embryogenic potential and somatic embryo maturation in Larix kaempferi (Lamb.) Carr [J]. PCTOC, 2013, 113 (1): 131-136

相似文献/References:

[1]邱栋梁,刘星辉,王湘平.模拟酸雨对龙眼叶绿体的伤害效应[J].应用与环境生物学报,2002,8(02):154.
　QIU Dongliang,et al..Injury effects of simulated acid rain on chloroplasts of longan leaves[J].Chinese Journal of Applied & Environmental Biology,2002,8(04):154.
[2]邱栋梁,刘星辉,郭素枝.模拟酸雨对龙眼幼果纤维素酶活性和内源激素含量的影响[J].应用与环境生物学报,2004,10(01):35.
　QIU Dongliang,et al..Effects of simulated acid rain on cellulase activity and contents of endogenous hormone in young fruit of longan[J].Chinese Journal of Applied & Environmental Biology,2004,10(04):35.
[3]林玉玲,赖钟雄.龙眼胚性愈伤组织Cu/Zn-SOD分子伴侣基因CCS的克隆及其在体胚发生过程中的表达分析[J].应用与环境生物学报,2012,18(03):351.[doi:10.3724/SP.J.1145.2012.00351]
　LIN Yuling,LAI Zhongxiong.Cloning of Copper Chaperone for Superoxide Dismutase Gene CCS from Embryogenic Callus of Dimocarpus longan Lour. and Its Expression Analysis During Somatic Embryogenesis[J].Chinese Journal of Applied & Environmental Biology,2012,18(04):351.[doi:10.3724/SP.J.1145.2012.00351]
[4]赖瑞联,林玉玲,赖钟雄.龙眼生长素受体基因TIR1的克隆及其与miR393互作关系[J].应用与环境生物学报,2016,22(01):95.[doi:10.3724/SP.J.1145.2015.05051]
　LAI Ruilian,LIN Yuling & LAI Zhongxiong**.Cloning of auxin receptor gene TIR1 and its interaction with miR393 in Dimocarpus longan Lour.[J].Chinese Journal of Applied & Environmental Biology,2016,22(04):95.[doi:10.3724/SP.J.1145.2015.05051]
[5]王 云 彭丽云 孙雪丽 高玉莹 陈晓慧 陈裕坤 林玉玲 赖钟雄**.龙眼Hsf基因家族全基因组鉴定及体胚发生过程中的表达分析*[J].应用与环境生物学报,2019,25(02):1.[doi:10.19675/j.cnki.1006-687x.2018.06004]
　WANG Yun,PENG Liyun,SUN Xueli,et al.Genome-wide identification of longan Hsf family members and their functional analysis during somatic embryogenesis in longan*[J].Chinese Journal of Applied & Environmental Biology,2019,25(04):1.[doi:10.19675/j.cnki.1006-687x.2018.06004]

更新日期/Last Update: 2017-08-25