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[1]陈旭,曾友竞,王嘉毅,等.龙眼miR159家族成员进化特性及时空表达[J].应用与环境生物学报,2017,23(04):602-608.[doi:10.3724/SP.J.1145.2017.03011]
 CHEN Xu,ZENG Youjing,WANG Jiayi,et al.Effect of main grain components on the starch swelling power of Tibetan hull-less barley (Hordeum vulgare var. nudum)[J].Chinese Journal of Applied & Environmental Biology,2017,23(04):602-608.[doi:10.3724/SP.J.1145.2017.03011]
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龙眼miR159家族成员进化特性及时空表达 ()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
23卷
期数:
2017年04期
页码:
602-608
栏目:
研究论文
出版日期:
2017-08-25

文章信息/Info

Title:
Effect of main grain components on the starch swelling power of Tibetan hull-less barley (Hordeum vulgare var. nudum)
作者:
陈旭曾友竞王嘉毅陈晓慧徐小萍李汉生张梓浩陈裕坤赖钟雄王天池林玉玲
福建农林大学园艺植物生物工程研究所 福州 350002
Author(s):
CHEN Xu ZENG Youjing WANG Jiayi CHEN Xiaohui XU Xiaoping LI Hansheng ZHANG Zihao CHEN Yukun LAI Zhongxiong WANG Tianchi** & LIN Yuling**
Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
关键词:
龙眼miR159家族进化特性实时荧光定量
Keywords:
Dimocarpus longan miR159 family evolutionary characteristic real-time quantitative PCR
分类号:
S667.203
DOI:
10.3724/SP.J.1145.2017.03011
摘要:
为了解植物miR159家族成员的进化特性及其在不同组织部位的表达规律,以龙眼miR159家族为例对miRNA家族进行探讨. 通过龙眼miR159家族前体与成熟体序列比对、前体二级结构预测、系统发育进化树的构建、靶基因预测及实时荧光定量等手段,对龙眼miR159基因家族进行研究. 结果显示:龙眼miR159家族存在7个前体成员和6个成熟体成员,序列比对发现5个成熟体可以高度定位于7个前体序列中一个共有的、约20个碱基的保守区域,推测miR159成熟体主要来源于该区域;进一步分析发现6个成熟体序列中仅2个可定位于pre-MIR159中. mfold预测结果显示miR159家族7个前体的最小折叠自由能在-56.54 - -92.53 kal/mol之间,均能自发形成典型、稳定的茎环二级结构. 系统发育进化树显示龙眼miR159家族前体与成熟体成员均具有保守性与多样性. 靶基因预测发现龙眼miR159家族所有成员共预测出14个不同靶标,其中3个成员也可以作用于同一靶标. 实时荧光定量PCR显示,龙眼pre-MIR159家族成员间在不同组织部位表达量差异显著,但表达趋势却几乎一致,其中花器官中表达量最高,提示了dlo-miR159在生殖器官形成过程中具有重要的作用,尤其是在雄花中作用显著. 本研究表明龙眼miR159家族在进化过程中形成了保守性与多样性并存的进化特性,并且可能在花器官形成过程中发挥了重要的作用. (图5 表3 参36)
Abstract:
In order to understand the evolutionary characteristics of miR159 family members and their expression patterns in different tissues, the miR159 family of Dimocarpus longan was used as an example in this study to explore its evolutionary characteristics. The evolutionary characteristics of miR159 gene family and its expression in different tissues of D. longan were studied by comparing the precursor and mature miRNA sequences, predicting their secondary structure, constructing a phylogenetic tree, target genes, and detecting their expression levels by real-time fluorescence quantification. The results showed that the D. longan miR159 family contained six mature miRNA sequences and seven precursor sequences; they shared a conserved region of about 20 bases, suggesting that mature miR159 mainly originated from the conserved region of the precursor sequences. Among of the six mature miRNA sequences, only two could be perfectly located in pre-MIR159s. The mfold prediction showed that the minimum free radical energy of the 7 precursors of miR159 family was between -56.54 kal/mol and -92.53 kal/mol; the pre-MIR159s could form a typical and stable stem-loop secondary structure. Phylogenetic tree showed that the precursors of the D. longan miR159 family have some conservatism and diversity. Target gene prediction results showed that D. longan miR159 family has 14 different targets, and multiple members can target the same genes. Real-time quantitative PCR showed that the expression of pre-MIR159 members was different in different tissues, which was higher in floral organs, but the expression trend of each member was consistent, suggesting that D. longan miR159 family was important in the process of gametogenesis, especially in male flowers. The evolutionary characteristics of D. longan miR159 family were conservative and diverse, and they may have an important role in the process of floral organ formation.

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更新日期/Last Update: 2017-08-25