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[1]陈敬,彭友林,张圣豪,等.长江上游地区杂交水稻亲本遗传多样性[J].应用与环境生物学报,2017,23(04):595-601.[doi:10.3724/SP.J.1145.2017.02028]
 CHEN Jing#,PENG Youlin#,ZHANG Shenghao,et al.QTL mapping of yield-related traits in the high-yield wheat variety Chuanmai42[J].Chinese Journal of Applied & Environmental Biology,2017,23(04):595-601.[doi:10.3724/SP.J.1145.2017.02028]
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长江上游地区杂交水稻亲本遗传多样性()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
23卷
期数:
2017年04期
页码:
595-601
栏目:
研究论文
出版日期:
2017-08-25

文章信息/Info

Title:
QTL mapping of yield-related traits in the high-yield wheat variety Chuanmai42
作者:
陈敬彭友林张圣豪卓驰夫罗丰唐阳杨国涛王学春陈永军吕启明胡运高
1西南科技大学水稻研究所 绵阳 621010 2湖南杂交水稻研究中心 长沙 410125
Author(s):
CHEN Jing1# PENG Youlin1# ZHANG Shenghao1 ZHUO Chifu1 LUO Feng1 TANG Yang1 YANG Guotao1 WANG Xuechun1 CHEN Yongjun1 L? Qiming2 & HU Yungao1**
1Rice Research Institute, Southwest University of Science and Technology, Mianyang 621010, China 2Hunan Hybrid Rice Research Center, Changsha 410125, China
关键词:
杂交水稻长江上游SSR标记遗传多样性群体结构
Keywords:
hybrid rice upper Yangtze River SSR marker genetic diversity population structure
分类号:
S511.03
DOI:
10.3724/SP.J.1145.2017.02028
摘要:
利用表型鉴定以及47对均匀分布于水稻12条染色体的SSR标记,对长江上游138个杂交水稻亲本进行检测,并采用遗传相似系数及数学模型分析其群体结构,以了解其遗传多样性信息. 结果显示,长江上游杂交水稻亲本的保持系、恢复系以及总体的表型性状遗传变异程度较大,适用于进行遗传多样性分析. 利用47对SSR标记共检测到94个等位基因位点,平均每个位点2个;其中有效等位基因数67.05个,占71.33%,Nei氏遗传多样性指数变幅为0-0.51,平均值为0.26. 供试材料可分为恢复系类群和保持系类群,与生产上利用的保持系和恢复系高度一致. 本研究表明利用SSR标记能详细了解长江上游杂交水稻亲本遗传多样性信息并有效区分恢复系与保持系. (图3 表2 参35)
Abstract:
In order to unveil genetic information of parents of hybrid rice from upper Yangtze River, the genetic diversity and structure of 138 parents of hybrid rice were determined by phenotypic identification, and evenly spaced 47 SSR markers. Population structure was analyzed by genetic similarity coefficient and mathematical modeling. Based on the phenotypes of rice accessions, the degree of genetic variation of maintainer lines, restorers, and total materials was high. There were 94 alleles, averaging 2 alleles per locus. The proportion of effective alleles was 71.33%, including 67.05 alleles. The range of Nei’s genetic diversity index was 0-0.5 with an average of 0.257. All parents of hybrid rice were divided into two groups, maintainer lines and the second group comprising of the restorer lines. This result was highly consistent with preassigned groups of maintainer and restorer lines. In conclusion, it is efficient to ascertain information of genetic diversity and distinguish parents of hybrid rice from upper Yangtze River based on SSR markers.

参考文献/References:

1 张涛, 杨蛟, 蒋开锋, 曹应江, 杨莉, 杨乾华, 万先齐, 游书梅, 罗婧, 高磊, 李昭祥, 郑家奎. 利用产量功能基因标记分析三系杂交水稻亲本的遗传多样性[J]. 中国农业科学, 2014, 47 (1): 11-23 [Zhang T, Yang J, Jiang KF, Cao YJ, Yang L, Yang QH, Wan XQ, You SM, Luo J, Li ZX, Zheng JK. Genetic diversity of the main Chinese three-line hybrid rice parents based on functional genetic markers related to yield [J]. Sci Agric Sin, 2014, 47 (1): 11-23]
2 孙健, 王敬国, 刘化龙, 杨亮, 赵雪, 谢冬微, 邹德堂. 黑龙江省主栽水稻品种的遗传多样性分析[J]. 作物杂志, 2011 (1): 63-67 [Sun J, Wang JG, Liu HL, Yang L, Zhao X, Xie DH, Zou DT. Analysis of genetic diversity of major rice cultivars in Heilongjiang Province [J]. Crops, 2011 (1): 63-67]
3 孙建昌, 曹桂兰, 李亚非, 马静, 陈耀锋, 韩龙植. 水稻地方品种群体内的遗传多样性分析[J]. 西北农林科技大学学报(自然科学版), 2011, 39 (12): 145-152, 158 [Sun JC, Cao GL, Li YF, Ma J, Chen YF, Han LZ.Analysis of genetic diversity within populations of rice (Oryza sativa L.) landraces [J]. J Northwest A&F Univ (Nat Sci Ed), 2011, 39 (12): 145-152, 158]
4 Souza E, Sorrells ME. Pedigree analysis of North American oat cultivars released from 1951 to 1985 [J]. Crop Sci, 1989, 29: 595-601
5 Almanzapinzon MI, Khairallah M, Fox PN, Warburton ML. Comparison of molecular markers and coefficients of parentage for the analysis of genetic diversity among spring bread wheat accessions. Euphytica, 2003, 130 (1): 77-86
6 Fufa H, Baenziger PS, Beecher Dweikat I, Graybosch RA, Eskridge KM. Comparison of phenotypic and molecular marker-based classifications of hard red winter wheat cultivars. Euphytica, 2005, 145: 133-146
7 Zhang DL, Zhang HL, Wang MX, Sun JL, Qi YW, Wang FM, Wei XH, Han LZ, Wang XK, Li ZC. Genetic structure and differentiation of Oryza sativa L. in China revealed by microsatellites. Theor Appl Genet, 119 (6): 1105-1117
8 Garris A, Tai TH, Coburn JR, Kresovich S, Mccouch SR. Genetic structure and diversity in Oryza sativa L. Genetics, 2005, 169 (3): 1631-1638
9 Wang MX, Zhang H, Zhang DL, Qi YW, Fan ZL, Li D, Pan DJ, Cao YS, Qiu ZE, Philip SY, Yang QW, Wang XF, Li ZC. Genetic structure of Oryza rufipogon Griff. in China. Heredity, 2008, 101 (6): 527-535
10 郭树春, 安玉麟, 李素萍, 孙瑞芬, 张艳芳, 张启辰, 闫素丽. DNA分子标记在作物种质资源中的应用进展[J]. 华北农学报, 2007, 22 (S3): 91-97 [Guo SC, An YL, Li SP, Sun RF, Zhang YF, Zhang QC, Yan SL. The advancements of DNA molecular markers’s application in crop germplasm resource [J]. Acta Agric Bor-Sin, 2007, 22 (S3): 91-97]
11 Mccouch SR, Chen XL, Panaud O, Temnykh S, Xu YB, Cho YG, Huang N, Takashige I, Blair MW. Microsatellite marker development, mapping and applications in rice genetics and breeding. Plant Mol Biol, 1997, 35: 89-99
12 Li C, Zhang Y, Ying K, Liang XL, Han B. Sequence variations of simple sequence repeats on chromosome-4 in two subspecies of the Asian cultivated rice. Theor Appl Genet, 108 (3): 392-400
13 栾丽, 孔繁伦, 何涛, 刘刚, 涂升斌. 用SSR标记检测同源四倍体与二倍体水稻的遗传差异[J]. 应用与环境生物学报, 2004, 10 (5): 556-558 [Luan L, Kong FL, He T, Liu G, Tu SB. Genetic variation of tetraploid and diploid rice revealed by microsatellite marks [J]. Chin J Appl Environ Biol, 2004, 10 (5): 556-558]
14 张立娜, 曹桂兰, 韩龙植. 利用SSR标记揭示中国粳稻地方品种遗传多样性[J]. 中国农业科学, 2012, 45 (3): 405-413 [Zhang LN, Cao GL, Han LZ. Analysis of genetic diversity of Japonica rice landrace in China with microsatellite marker [J]. Sci Agric Sin, 2012, 45 (3): 405-413]
15 田洁, 罗科. 水稻总DNA的快速制备[J]. 应用与环境生物学报, 2004, 10 (2): 143-145 [Tian J, Luo K. Fast and reliable extraction processes of total DNAs from rice [J]. Chin J Appl Environ Biol, 2004, 10 (2): 143-145]
16 Gao LZ, Schaal BA, Zhang CH, Jia JZ, Dong YS. Assessment of population genetic structure in common wild rice Oryza rufipogon Griff. using microsatellite and allozyme markers. Theoret Appl Genet, 2002, 106 (1): 173-180
17 Gao L. Population structure and conservation genetics of wild rice Oryza rufipogon (Poaceae): a region-wide perspective from microsatellite variation [J]. Molr Ecol, 2004, 13: 1009-1024
18 Gao LZ, Zhang CH, Li DY, Pan DJ, Jia JZ, Dong YS. Genetic diversity within Oryza rufipogon germplasms preserved in Chinese field gene banks of wild rice as revealed by microsatellite markers. Biodiv Conserv, 15 (13): 4059-4077
19 王振山, 陈洪, 朱立煌, 王象坤. 中国普通野生稻遗传分化的RAPD研究[J]. 植物学报, 1996, 38 (9): 749-752 [Wang ZS, Chen H, Zhu LH, Wang XK. RAPD determinations on the genetic differentiation of Chinese common wild rice [J]. Acta Bot Sin, 1996, 38 (9): 749-752]
20 王振山, 朱立煌, 刘志勇, 王象坤. 野生稻天然群体限制性酶切片段长度(RFLP)多态性研究[J]. 农业生物技术学报, 1996, 4 (2): 11-17 [Wang ZS, Zhu LH, Liu ZY, Wang XK. Gene diversity of natural wild rice populations detected by RFLP markers [J]. J Agric Biotechnol, 1996, 4 (2): 11-17]
21 孙传清, 王象坤, 吉村淳, 岩田伸夫. 普遍野生稻和亚洲栽培稻遗传多样性的研究[J]. 遗传学报, 2000, 27 (3): 227-234 [Sun CQ, Wang XK, Ji CC, Yoshimura A. A study of the genetic diversity of common wild rice (O. rufipogon Griff.) and cultivated rice (O. sativa) by RFLP analysis [J]. Acta Genet Sin, 2000, 27 (3): 227-234]
220 张冬玲, 张洪亮, 齐永文, 王美兴, 孙俊立, 丁立, 李自超. 中国籼稻亚种内的群体结构及地理生态分化[J]. 科学通报, 2012, 57 (4): 248-257 [Zhang DL, Zang HL, Qi YW, Wang MX, Sun JL, Ding L, Li ZC. Genetic structure and eco-geographical differentiation of cultivated Hsien rice (Oryza sativa L. subsp. indica) in China revealed by microsatellites [J]. Sci Bull, 2012, 57 (4): 248-257]
23 李红宇, 侯昱铭, 陈英华, 徐正进, 陈温福, 赵明辉, 马殿荣, 徐海, 王嘉宇. 用SSR标记评估东北三省水稻推广品种的遗传多样性[J]. 中国水稻科学, 2009, 23 (4): 383-390 [Li HY, Hou YM, Chen YH, Xu ZJ, Chen FW, Zhao MH, Ma DR, Xu H, Wang JY. Evaluation on genetic diversity of the commercial rice varieties in Northeast China by microsatellite markers [J]. Chin J Rice Sci, 2009, 23 (4): 383-390]
24 涂敏, 王云月, 卢宝荣, 董树斌. 云南省不同地理位置水稻品种遗传多样性分析[J]. 热带作物学报, 2011, 32 (6): 998-1003 [Tu M, Wang YY, Lu BR, Dong SB. Correlation between blast resistance difference and genetic diversity of rice varieties in Yunnan Province [J]. Chin J Trop Crops, 2011, 32 (6): 998-1003]
25 马文东. 水稻基因组DNA提取方法的研究[J]. 黑龙江农业科学, 2014 (1): 7-11 [Ma WD. Study on extraction medthod of rice genomic DNA [J]. Heilongjiang Agric Sci, 2014 (1): 7-11]
26 刘承晨, 赵富伟, 吴晓霞, 张昌泉, 朱孔志, 薛达元, 武建勇, 黄绍文, 徐小颖, 金银根, 刘巧泉. 云南哈尼梯田当前栽培水稻遗传多样性及群体结构分析[J]. 中国水稻科学, 2015, 29 (1): 28-34 [Liu CC, Zhao FW, Wu XX. Zhang CQ, Zhu KZ, Xue DY, Wu JY, Huang SW, Xu XY, Jin YG, Liu QQ. Genetic diversity and population structure analysis of currently cultivated rice landraces form Hani’s terraced fields in Yunnan Province [J]. Chin J Rice Sci, 2015, 29 (1): 28-34]
27 董超, 徐福荣, 杨文毅, 汤翠凤, 张恩来, 杨雅云, 阿新祥, 张斐斐, 卢光德, 王艳, 戴陆园. 云南元阳哈尼梯田水稻地方品种月亮谷的遗传变异分析[J]. 中国水稻科学, 2013, 27 (2): 137-144 [Dong C, Xu FR, Yang WY, Tang CP, Zhang EL, Yang YY, A XX, Zhang FF, Lu GD, Wang Y, Dai LY .Genetic variation analysis of paddy rice landrace of Yuelianggu from Yuanyang Hani’s terraced fields in Yunnan Province [J]. Chin J Rice Sci, 2013, 27 (2): 137-144]
28 刘承晨. 云南元阳哈尼梯田现有栽培水稻遗传多样性分析[D]. 扬州: 扬州大学, 2015 [Liu CC. Genetic diversity analysis of currently cultivated rice landraces form Hani’s terraced fields in Yunnan [D]. Yangzhou: Yangzhou University, 2015]
29 Wright S. Evolution and the Genetics of Populations. Vol 3. Experimental Results and Evolutionary Deductions [M]. Chicago: University of Chicago Press, 1968
30 Wang SJ, Wan JM, Lu ZM. Parental cluster analysis in indica hybrid rice (Oryza sativa L.) by SSR analysis [J]. Acta Agron Sin, 2006, 10: 1437-1443]
31 马旭丹. 水稻部分骨干亲本的指纹图谱构建和遗传多样性分析[D]. 武汉: 华中农业大学, 2015 [Ma XD. Analysis of genetic polymorphism and fingerprinting of some backbone rice parents [D]. Wuhan: Huazhong Agricultural University, 2015]
32 应杰政, 施勇烽, 庄杰云, 薛庆中. 用微卫星标记评估中国水稻主栽品种的遗传多样性[J]. 中国农业科学, 2007, 40 (4): 649-654 [Ying ZJ, Shi YF, Zhuang JY, Xue QZ. Microsatellite marker evaluation on genetic diversity of the major commercial rice varieties in China [J]. Sci Agric Sin, 2007, 40 (4): 649-654]
33 钟超. 籼稻不育系与恢复系的单核苷酸多态性[D]. 南昌: 南昌大学, 2012 [Zhong C. Single nucleotide polymorphisms of male sterile lines and restorer lines in Oryza sativa L. ssp. indica [D]. Nanchang: Nanchang University, 2012]
34 赖勇, 王鹏喜, 范贵强, 司二静, 王晋, 杨轲, 孟亚雄, 李葆春, 马小乐, 尚勋武, 王化俊. 大麦SSR标记遗传多样性及其与农艺性状关联分析[J]. 中国农业科学, 2013, 46 (2): 233-242 [Lai Y, Wang PX, Fan GQ, Si EJ, Wang J, Yang K, Meng YX, Li BC, Ma XL, Shang XW, Wang HJ. Genetic diversity and association analysis using SSR markers in barley [J]. Sci Agric Sin, 2013, 46 (2): 233-242]
35 陈英, 邱琳, 涂升斌, 栾丽, 王兴, 龙文波. 用SSR标记检测杂交籼稻三系亲本的遗传差异[J]. 应用与环境生物学报, 2009, 15 (5): 585-590 [Chen J, Qiu L, Tu SB, Luan L, Wang X, Long WB. Genetic variation of three-line parents of indica hybrid rice detected with SSR markers [J]. Chin J Appl Environ Biol, 2009, 15 (5): 585-590]

更新日期/Last Update: 2017-08-25