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[1]颜朗,吴燕,陈鼎,等.皇竹草转录组分析揭示其抗冻特性[J].应用与环境生物学报,2017,23(03):459-466.[doi:2016.07038]
　YAN Lang,WU Yan,CHEN Ding,et al.Transcriptome analysis reveals cold acclimation in Pennisetum sinese Roxb[J].Chinese Journal of Applied & Environmental Biology,2017,23(03):459-466.[doi:2016.07038]
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皇竹草转录组分析揭示其抗冻特性()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
23卷
期数:
2017年03期
页码:
459-466
栏目:
研究论文
出版日期:
2017-06-25

文章信息/Info

Title:
Transcriptome analysis reveals cold acclimation in Pennisetum sinese Roxb
作者:
颜朗; 吴燕; 陈鼎; 谭雪梅; 张义正
1四川大学生命科学学院四川省分子生物学与生物技术重点实验室 成都 610064 2成都柯安科技有限公司 成都 610064
Author(s):
YAN Lang;  WU Yan;  CHEN Ding;  TAN Xuemei & ZHANG Yizheng
1Sichuan Key Laboratory of Molecular Biology and Biotechnology, College of Life Sciences, Sichuan University, Chengdu 610064, China 2Chengdu Ke’an Technology Ltd., Chengdu 610064, China
关键词:
皇竹草; 转录组分析; 抗冻基因; 基因差异表达; 功能聚类; 低温胁迫应答
Keywords:
Pennisetum sinese Roxb;  transcriptome analysis;  cold resistant gene;  gene differential expression;  functional cluster;  low temperature stress response
分类号:
S543.903.4
DOI:
2016.07038
摘要:
皇竹草是一种高产、优质牧草，但因耐寒性较差而极大限制了其在我国大面积种植和推广. 选择在冬季平均温度接近皇竹草耐寒极限温度的四川地区进行了5年自然驯化，筛选在最低温度-5 ℃下能够自然过冬的样本，并在冬季最低温度下和夏季最高温度下分别取样进行转录组测序，挖掘皇竹草本身具有的耐寒、抗冻基因. 结果显示：皇竹草转录组共有转录本125 467条，注释转录本65 633条，注释信息及邻近分析显示皇竹草与小米、玉米高度同源. 冬夏两季皇竹草比较转录组分析共计筛选差异表达基因11 443个，其中冬季相对于夏季表达量上调基因6 943个，下调基因4 500个，差异显著. 在以上差异表达基因中，共计挖掘在冬季低温条件下高度表达的抗冻基因112个，且有17个抗冻基因在冬季样品中特异表达. 随机选取6个差异表达的抗冻基因进行荧光定量PCR实验验证，结果显示与预测转录本表达量一致. 本研究对皇竹草进行了转录组测序并通过比较转录组的方法挖掘其在自然驯化条件下成功越冬样本中的抗冻基因，有利于揭示皇竹草冷适应下的抗性机制，同时也有助于热带引种植物分子育种的开展. （图5 表4 参29）
Abstract:
Pennisetum sinese Roxb is a high-quality and high-yield grass from South America. Because of its high propagation efficiency, forage palatability, and nutritional value, P. sinese could be widely used as livestock feed. However, it does not naturally hibernate in northern regions because of it low cold resistance, which restricts its growth and spread across China. In this study, the natural domestication of P. sinese for 5 years in Sichuan province, where the mean winter temperature is close to the sample cold-resistance limit, was analyzed, samples of plants that survived at ?5 ℃ in the winter were identified, and whole transcriptomes of plants collected at the lowest temperature in winter and highest temperature in summer were sequenced. The transcriptome of P. sinese comprised 125 467 transcripts, of which 65 633 had been annotated. Annotation and proximity analyses showed that the transcriptome of P. sinese is highly similar to those of millet (Setaria italica) and maize (Zea mays). There were 11 443 differentially expressed genes (DEGs) identified through comparative transcriptome analysis, with 6 943 genes significantly upregulated in the winter relative to those upregulated in the summer, and 4 500 genes significantly downregulated. Of all of the DEGs, there were 112 cold-tolerance genes that were highly expressed in winter, 17 of which were specifically expressed in these conditions. Quantitative PCR was used to verify the expression levels of six randomly selected differentially expressed cold-tolerance genes, and the results showed that their expression levels were consistent with those predicted. In this study, the transcriptomes of natural domesticated P. sinese were sequenced, and cold-tolerance genes were identified in plants that had successfully overwintered. These results contribute to an understanding of the cold adaptation mechanisms of P. sinese and inform the breeding of introduced tropical plants.

参考文献/References:

1 李海利, 齐大胜, 张潇月, 谷子林. 栽培技术对皇竹草产量和品质的影响[J]. 饲料博览, 2014 (6): 26-29 [Li HL, Qi DS, Zhang XY, Gu ZL. Effects of cultivation techniques on yield and forage quality of hybrid giant napier [J]. Feed Rev, 2014, (6): 26-29]
2 Pantulu JV. Pachytene pairing and meiosis in the F1 hybrid of Pennisetum typhoides and P. purpureum [J]. Cytologia, 1967, 32 (3-4): 532-541
3 Dos Reis GB, Mesquita AT, Torres GA, Andrade-Vieira LF, Vander Pereira A, Davide LC. Genomic homeology between Pennisetum purpureum and Pennisetum glaucum (Poaceae) [J]. Compar Cytogenet, 2014, 8(3): 199
4 Martel E, Poncet V, Lamy F, Siljak-Yakovlev S, Lejeune B, Sarr A. Chromosome evolution of Pennisetum species (Poaceae): implications of ITS phylogeny [J]. Plant Syst Evol, 2004, 249 (3-4): 139-149
5 卓锦辉. 草中之王——皇竹草[J]. 福建农业, 2007 (6): 16-16
6 张晓佩, 高承芳, 刘远, 陈鑫珠, 李文杨, 董晓宁. 皇竹草的综合开发利用[J]. 农业开发与装备, 2015 (12): 34
7 郑海, 陈小华, 黎健龙，黎华寿. 水分胁迫对皇竹草形态及叶片光合特性的影响[J]. 广东农业科学, 2014, 41 (13): 25-28 [Zheng H, Chen XH, Li JL, Li HS. Effects of water stress on morphological and photosynthetic characteristics of hybrid giant napier (Pennisetum hydridum) [J]. Guangdong Agric Sci, 2014, 41 (13): 25-28]
8 孔嫣, 伊亚莉, 刘树军. 多功能牧草“皇竹草”耐寒性研究[J]. 当代畜牧, 2015 (15): 55-56
9 彭乃木, 胡文胜, 李金顺, 欧海敏, 张仁喜, 金大春. 青贮皇竹草替代稻草饲喂育肥牛增重效果试验[J]. 中国草食动物, 2010, 30 (5): 84-85
10 刘松柏, 李攀峰, 付海涵, 王治喜, 彭洪翠. 青贮皇竹草饲喂奶牛效果分析[J]. 中国牛业科学, 2008, 34 (3): 41-44 [Liu SB, Li PF, Fu HH, Peng HC. Analysis of the efficiency of feeding hybrid giant napier silage to dairy cows [J]. China Cattle Sci, 2008, 34 (3): 41-44]
11 詹宽文, 唐书辉, 汪庆松, 王海凤, 王兴磊, 贺起凯. 皇竹草与玉米秸秆青贮料饲喂肉牛的对比试验[J]. 贵州畜牧兽医, 2014 (5): 9-11 [Zhan KW, Tang SH, Wang QS, Wang HF, Wang XL, He QK. Contrast test of Pennisetum hydridum and corn straw mixed silage feeding beef cattle [J]. Guizhou Anim Sci Veter Med, 2014 (5): 9-11]
12 孔嫣, 刘树军, 伊亚莉. 皇竹草的不同利用方式及效益分析[J]. 贵州畜牧兽医, 2015, 39 (3): 62-63 [Kong Y, Liu SJ, Yi YL. Different utilization ways and benefit analysis of imperial bamboo grass [J]. Guizhou Anim Sci Veter Med, 2015, 39 (3): 62-63]
13 贲正坤. 四川省奶牛的杂交育种[J]. 中国奶牛, 1989 (6): 31-32
14 毛春英. 香樟的引种与驯化研究[J]. 山东农业大学学报: 自然科学版, 2004, 35 (4): 534-539 [Mao CY. Research of introduction and domestication of Cinnamomum camphora (Linn) Presl [J]. J Shandong Agric Univ (Nat Sci), 2004, 35 (4): 534-539]
15 Ashburner M, Ball CA, Blake JA, Botstein D, Butler H, Cherry JM, Davis AP, Dolinski K, Dwight SS, Eppig JT, Harris MA. Gene ontology: tool for the unification of biology. Nat Genet, 2000, 25: 25-29
16 Kanehisa M, Goto S, Kawashima S, Okuno Y, Hattori M. The KEGG resource for deciphering the genome. Nucleic Acids Res, 2004, 32: D277-D280
17 Robinson MD, McCarthy DJ, Smyth GK. edgeR: a Bioconductor package for differential expression analysis of digital gene expression data. Bioinformatics, 2010, 26: 139-140
18 Baid VW. Low temperature and drought regulated gene expression in bermudagrass. Turfgrass Environ Res, 1994: 32-33
19 杨广东, 张战备, 郭瑜敏, 赵鸿钧. 脂肪酸与青椒苗期和花期抗冷性关系[J]. 北方园艺, 1999 (4): 1-2
20 李合生. 现代植物生理学[M]. 2版. 北京: 高等教育出版社, 2002: 352-256
21 谭振波, 刘昕, 曹鸣庆. 玉米抗寒性的研究进展. 玉米科学, 2002, 10 (2): 56-60 [Tan ZB, Liu X, Cao MQ. Recent advances on mechanisms of maize chilling tolerance. J Maize Sci, 2002, 10 (2): 56-60]
22 罗军武, 唐和平, 黄意欢, 龚志华, 肖文军. 茶树不同抗寒性品种间保护酶类活性的差异. 湖南农业大学学报, 2001, 27 (2): 94-96 [Luo JW, Tang HP, Huang YH, Gong ZH, Xiao WJ. Differences of activities of protective enzymes of tea plant varieties with different cold resistant abilities [J]. J Hunan Agric Univ (Nat Sci), 2001, 27 (2): 94-96]
23 王小华, 庄南生. 脯氨酸与植物抗寒性的研究进展. 中国农学通报, 2008, 24 (11): 398-402 [Wang XH, Zhuang NS. Advances in research on proline and cold resistance of plant [J]. Chin Agric Sci Bull, 2008, 24 (11): 398-402]
24 Borman HC, Janshan E. Nieotiananata bacum callus studies ABA increase resistance to cold damage. Physiol Plant, 1980, 48: 491-493
25 Gilmour SJ, Sebolt AM, Salazar MP, Everard JD, Thomashow MF. Overexpression of the Arabidopsis CBF3 transcriptional activator mimics multiple biochemical changes associated with cold acclimation. Plant Physiol, 2000, 124: 1854-1865
26 王淑杰, 王家民, 李亚东, 王春梅. 可溶性全蛋白、可溶性糖含量与葡萄抗寒性关系的研究[J]. 北方园艺, 1996 (2): 13-14
27 孙玉洁, 王国槐. 植物抗寒生理的研究进展. 作物研究, 2009, 23 (5): 293
28 王兴, 于晶, 杨阳, 苍晶, 李卓夫. 低温条件下不同抗寒性冬小麦内源激素的变化. 麦类作物学[J], 2009, 29 (5): 827-831 [Wang X, Yu J, Yang Y, Cang J, Li ZF. Changes of endogenous hormones of winter wheat varieties with different cold-resistances under low temperature [J]. J Triticeae Crops, 2009, 29 (5): 827-831]
29 Yamaguchi-Shinozaki K, Shinozaki K. Transeriptional regulatory networks in cellular response and tolerance to dehydration and cold stresses. Annu Rev Plant Biol, 2006, 57: 781-803

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更新日期/Last Update: 2017-06-25