|本期目录/Table of Contents|

[1]张毓森,叶军,苏建强.粪肥与铜一次性施用对农田土壤抗生素抗性基因的长期影响[J].应用与环境生物学报,2019,25(02):328-332.[doi:10.19675/j.cnki.1006-687x.2018.06020]
 ZHANG Yusen,YE Jun & SU Jianqiang**.Long-term effect of one-time application of manure or copper on antibiotic resistance genes in agricultural soil[J].Chinese Journal of Applied & Environmental Biology,2019,25(02):328-332.[doi:10.19675/j.cnki.1006-687x.2018.06020]
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粪肥与铜一次性施用对农田土壤抗生素抗性基因的长期影响
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年02期
页码:
328-332
栏目:
研究论文
出版日期:
2019-04-25

文章信息/Info

Title:
Long-term effect of one-time application of manure or copper on antibiotic resistance genes in agricultural soil
作者:
张毓森叶军苏建强
1中国科学院城市环境研究所 厦门 361021 2中国科学院大学 北京 100049
Author(s):
ZHANG Yusen1 2 YE Jun1 & SU Jianqiang1**
1 Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China 2 University of Chinese Academy of Sciences, Beijing 100049, China
关键词:
抗生素抗性基因粪肥高通量定量聚合酶链式反应农田土壤
Keywords:
antibiotic resistance gene manure copper high-throughput quantitative polymerase chain reaction agricultural soil
分类号:
X171.1 : S151.4
DOI:
10.19675/j.cnki.1006-687x.2018.06020
摘要:
人类农业生产活动显著影响农田生态系统中抗生素抗性基因的增殖与扩散,为探究粪肥与铜施加对土壤抗生素抗性基因增殖与传播的长期影响,采用高通量荧光定量聚合酶链式反应分析猪粪和铜停施10年后土壤中抗生素抗性基因多样性和丰度. 结果显示:猪粪和铜停施10年后,土壤抗生素抗性基因相对丰度仍分别处于2.16倍和2.01倍于对照组的较高水平,说明猪粪和铜施用可导致土壤抗生素抗性基因的长期存在,其中ycel_mdtH和cphA基因在猪粪和铜施用两种处理中均显著富集. PCA结果显示,猪粪和铜处理土壤中抗生素抗性基因的分布格局无明显差别,铜处理土壤中所检出的抗生素抗性基因数量的90%以上均可在猪粪施用土壤中检出,而铜是唯一在两种处理土壤中检出浓度均显著高于(P < 0.001)对照并处于同一浓度水平的元素. 本研究表明铜可能是导致土壤中抗生素抗性基因增殖扩散的主要因素之一,施加重金属含量较高的粪肥对农田土壤抗生素抗性基因有着长期影响的风险;结果可为农业中的粪肥施用提供新的风险评价参数. (图4 参26)
Abstract:
The proliferation and spread of antibiotic resistance genes (ARGs) in the agricultural ecosystem have been significantly influenced by human agricultural activities, including the application of heavy metals and animal manure. However, since previous studies mainly focus on the impact of a periodical application of manures on ARGs in the soil, the persistence of ARGs after a one-time application of manure and heavy metals remain poorly understood. In this study, a high-throughput quantitative polymerase chain reaction (qPCR) was performed to investigate the diversity and relative abundance of ARGs in long-term experimental soils, which had been treated by manure or copper ten years ago. The results showed that a higher relative abundance of ARGs remained after suspending treatment for ten years. The relative abundance of ARGs were 2.16 and 2.01 times higher than that in the control soil, indicating the persistence and enrichment of ARGs in manure- or copper-treated soil, especially for the ycel_mdtH and cphA genes. Principal component analysis showed similar ARG profiles between manure treatments and copper treatments as 90% of the detected ARGs were shared between both treatments. Additionally, copper was the unique element that was frequently detected from both manure and copper treatments, suggesting that copper might be one of the major factors that influence the enrichment of ARGs in the soil. The results revealed the long-term risk of applying metal-enriched manure into agricultural soil, providing new parameters for the assessment of manure applications in agriculture.

参考文献/References:

1 Zhu YG, Zhao Y, Li B, Huang CL, Zhang SY, Yu S, Chen YS, Zhang T, Gillings MR, Su JQ. Continental-scale pollution of estuaries with antibiotic resistance genes [J]. Nat Microbiol, 2017, 2: 16270
2 Hu Y, Gao GF, Zhu B. The antibiotic resistome: gene flow in environments, animals and human beings [J]. Fro Med, 2017, 11 (2): 161-168
3 Chen B, Yang Y, Liang X, Yu K, Zhang T, Li X. Metagenomic profiles of antibiotic resistance genes (ARGs) between human impacted estuary and deep ocean sediments [J]. Enviro Sci Technol, 2013, 47 (22): 12753-12760
4 黄福义, 李虎, 安新丽, 欧阳纬莹, 苏建强. 城市生活污水和生活垃圾渗滤液抗生素抗性基因污染的比较研究[J]. 环境科学, 2016 (10): 3949-3954 [Huang FY, Li H, An XL, Ouyang WY, Su JQ. Comparative investigation of antibiotic resistance genes between wastewater and landfill leachate [J]. Environ Sci, 2016 (10): 3949-3954]
5 Zhu YG, Johnson TA, Su JQ, Qiao M, Guo GX, Stedtfeld RD, Hashsham SA, Tiedje JM. Diverse and abundant antibiotic resistance genes in Chinese swine farms [J]. PNAS, 2013, 110 (9): 3435-3440
6 黄福义, 李虎, 韦蓓, 欧阳纬莹, 苏建强. 长期施用猪粪水稻土抗生素抗性基因污染研究[J]. 环境科学, 2014 (10): 3869-3873 [Huang FY, Li H, Wei B, Ouyang WY, Su JQ. Long-term manure application induced shift of diversity and abundance of antibiotic resistance genes in paddy soil [J]. Environ Sci, 2014 (10): 3869-3873]
7 Su JQ, Wei B, OuYang WY, Huang FY, Zhao Y, Xu HJ, Zhu YG. Antibiotic resistome and Its association with bacterial communities during sewage sludge composting [J]. Environ Sci Technol, 2015, 49 (12): 7356-7363
8 Sentchilo V, Mayer AP, Guy L, Miyazaki R, Green Tringe S, Barry K, Malfatti S, Goessmann A, Robinson-Rechavi M, van der Meer JR. Community-wide plasmid gene mobilization and selection [J]. ISME J, 2013, 7 (6): 1173-1186
9 Berg J, Thorsen MK, Holm PE, Jensen J, Nybroe O, Brandt KK. Cu exposure under field conditions coselects for antibiotic resistance as determined by a novel cultivation-independent bacterial community tolerance assay [J]. Environ Sci Technol, 2010, 44 (22): 8724-8728
10 Hu HW, Wang JT, Li J, Shi XZ, Ma YB, Chen D, He JZ. Long-term nickel contamination increases the occurrence of antibiotic resistance genes in agricultural soils [J]. Environ Sci Technol, 2017, 51 (2): 790-800
11 Sarmah AK, Meyer MT, Boxall ABA. A global perspective on the use, sales, exposure pathways, occurrence, fate and effects of veterinary antibiotics (VAs) in the environment [J]. Chemosphere, 2006, 65 (5): 725-759
12 张俊亚, 魏源送, 陈梅雪, 佟娟, 熊继海, 敖子强. 畜禽粪便生物处理与土地利用全过程中抗生素和重金属抗性基因的赋存与转归特征研究进展[J]. 环境科学学报, 2015, 35 (4): 935-946 [Zhang JY, Wei YS, Chen MX, Tong J, Xiong JH, Ao ZQ. Occurrence and fate of antibiotic and heavy metal resistance genes in the total process of biological treatment and land application of animal manure [J]. Aata Sci Circumst, 2015, 35 (4): 935-946]
13 张树清, 张夫道, 刘秀梅, 王玉军, 邹绍文, 何绪生. 规模化养殖畜禽粪主要有害成分测定分析研究[J]. 植物营养与肥料学报, 2005, 11 (6): 116-123 [Zhang SQ, Zhang FD, Liu XM, Wang YJ, Zou SW, He XS, Determination and analysis on main harmful composition in excrement of scale livestock and poultry feedlots [J]. Plant Nutr Fert Sci, 2005, 11 (6): 822-829]
14 Hu HW, Wang JT, Li J, Li JJ, Ma YB, Chen D, He J-Z. Field-based evidence for copper contamination induced changes of antibiotic resistance in agricultural soils [J]. Environ Microbiol, 2016, 18 (11): 3896-3909
15 Huang CH, Renew J, Smeby K, Pinkston K, Sedlak D. Assessment of potential antibiotic contaminants in water and preliminary occurrence analysis [J]. J Contemp Water Res Educ, 2001, 120 (1): 30-40
16 沈怡雯, 黄智婷, 谢冰. 抗生素及其抗性基因在环境中的污染、降解和去除研究进展[J]. 应用与环境生物学报, 2015, 21 (2): 181-187 [Shen YW, Huang ZT, Xie B. Advances in research of pollution, degradation and removal of antibiotics and antibiotic resistance genes in the environment [J]. Chin J Appl Environ Biol, 2015, 21 (2): 181-187]
17 黄锦孙. 土壤铜镍植物毒害的室内和田间实验差异研究[D]. 北京: 中国农业科学院, 2012 [Huang JS. Differences between laboratory and field tests for phytotoxicity of copper and nickel in soils [D]. Beijing: Chinese Academy Agriculture Science, 2012]
18 章明奎, 何振立. 红壤类土壤中水磷铝铅矿类矿物的X射线衍射鉴定[J]. 土壤通报, 1998 (5): 5-6+20 [Zhang MK, He ZL. X-ray diffraction spectra detect the water phosphorus lead from red soils [J]. Chin J Soil Sci, 1998 (5): 5-6+20]
19 罗松英, 曹建劲, 吴政权. 内蒙古草原拜仁达坝矿区地表土壤的X射线衍射和近红外光谱分析[J]. 光谱学与光谱分析, 2014 (8): 2268-2272 [Luo SY, Cao JJ, Wu ZQ. X-ray diffraction and near infrared spectrum analysis of the soil overlying the airendba deposit of the inner mongolia grassland [J].Spec Spectl Anal, 2014 (8): 2268-2272]
20 Chen Q, An X, Li H, Su J, Ma Y, Zhu YG. Long-term field application of sewage sludge increases the abundance of antibiotic resistance genes in soil [J]. Environ Intl, 2016, 92-93: 1-10
21 Wang FH, Qiao M, Su JQ, Chen Z, Zhou X, Zhu YG. High throughput profiling of antibiotic resistance genes in urban park soils with reclaimed wateri rrigation [J]. Environ Sci Technol, 2014, 48 (6): 9079-9085
22 Looft T, Johnson TA, Allen HK, Bayles DO, Alt DP, Stedtfeld RD, Sul WJ, Stedtfeld TM, Chai B, Cole JR, Hashsham SA, Tiedje JM, Stanton TB. In-feed antibiotic effects on the swine intestinal microbiome [J]. PNAS, 2012, 109 (5): 1691-1696
23 Berg J, Tom-Petersen A, Nybroe O. Copper amendment of agricultural soil selects for bacterial antibiotic resistance in the field [J]. Lett Appl Microbiol, 2005, 40 (2): 146-151
24 Baker-Austin C, Wright MS, Stepanauskas R, McArthur JV. Co-selection of antibiotic and metal resistance [J]. Tren Microbiol, 2006, 14 (4): 176-182
25 Chee-Sanford JC, Mackie RI, Koike S, Krapac IG, Lin Y-F, Yannarell AC, Maxwell S, Aminov RI. Fate and transport of antibiotic residues and antibiotic resistance genes following land application of manure waste[J]. J Environ Qual, 2009, 38 (3): 1086-1108
26 Yin Y, Gu J, Wang X, Song W, Zhang K, Sun W, Zhang X, Zhang Y, Li H. Effects of copper addition on copper resistance, antibiotic resistance genes, and intl1 during swine manure composting [J]. Front Microbiol, 2017, 8: 344

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更新日期/Last Update: 2019-04-25