|本期目录/Table of Contents|

[1]张毓森,叶军,苏建强.粪肥与铜一次性施用对农田土壤抗生素抗性基因的长期影响[J].应用与环境生物学报,2019,25(02):328-332.[doi:10.19675/j.cnki.1006-687x.2018.06020]
　ZHANG Yusen,YE Jun & SU Jianqiang**.Long-term effect of one-time application of manure or copper on antibiotic resistance genes in agricultural soil[J].Chinese Journal of Applied & Environmental Biology,2019,25(02):328-332.[doi:10.19675/j.cnki.1006-687x.2018.06020]
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粪肥与铜一次性施用对农田土壤抗生素抗性基因的长期影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年02期
页码:
328-332
栏目:
研究论文
出版日期:
2019-04-25

文章信息/Info

Title:
Long-term effect of one-time application of manure or copper on antibiotic resistance genes in agricultural soil
作者:
张毓森; 叶军; 苏建强
1中国科学院城市环境研究所 厦门 361021 2中国科学院大学 北京 100049
Author(s):
ZHANG Yusen1;  2;  YE Jun1 & SU Jianqiang1**
1 Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China 2 University of Chinese Academy of Sciences, Beijing 100049, China
关键词:
抗生素抗性基因; 粪肥; 铜; 高通量定量聚合酶链式反应; 农田土壤
Keywords:
antibiotic resistance gene;  manure;  copper;  high-throughput quantitative polymerase chain reaction;  agricultural soil
分类号:
X171.1 : S151.4
DOI:
10.19675/j.cnki.1006-687x.2018.06020
摘要:
人类农业生产活动显著影响农田生态系统中抗生素抗性基因的增殖与扩散，为探究粪肥与铜施加对土壤抗生素抗性基因增殖与传播的长期影响，采用高通量荧光定量聚合酶链式反应分析猪粪和铜停施10年后土壤中抗生素抗性基因多样性和丰度. 结果显示：猪粪和铜停施10年后，土壤抗生素抗性基因相对丰度仍分别处于2.16倍和2.01倍于对照组的较高水平，说明猪粪和铜施用可导致土壤抗生素抗性基因的长期存在，其中ycel_mdtH和cphA基因在猪粪和铜施用两种处理中均显著富集. PCA结果显示，猪粪和铜处理土壤中抗生素抗性基因的分布格局无明显差别，铜处理土壤中所检出的抗生素抗性基因数量的90%以上均可在猪粪施用土壤中检出，而铜是唯一在两种处理土壤中检出浓度均显著高于（P < 0.001）对照并处于同一浓度水平的元素. 本研究表明铜可能是导致土壤中抗生素抗性基因增殖扩散的主要因素之一，施加重金属含量较高的粪肥对农田土壤抗生素抗性基因有着长期影响的风险；结果可为农业中的粪肥施用提供新的风险评价参数. （图4 参26）
Abstract:
The proliferation and spread of antibiotic resistance genes (ARGs) in the agricultural ecosystem have been significantly influenced by human agricultural activities, including the application of heavy metals and animal manure. However, since previous studies mainly focus on the impact of a periodical application of manures on ARGs in the soil, the persistence of ARGs after a one-time application of manure and heavy metals remain poorly understood. In this study, a high-throughput quantitative polymerase chain reaction (qPCR) was performed to investigate the diversity and relative abundance of ARGs in long-term experimental soils, which had been treated by manure or copper ten years ago. The results showed that a higher relative abundance of ARGs remained after suspending treatment for ten years. The relative abundance of ARGs were 2.16 and 2.01 times higher than that in the control soil, indicating the persistence and enrichment of ARGs in manure- or copper-treated soil, especially for the ycel_mdtH and cphA genes. Principal component analysis showed similar ARG profiles between manure treatments and copper treatments as 90% of the detected ARGs were shared between both treatments. Additionally, copper was the unique element that was frequently detected from both manure and copper treatments, suggesting that copper might be one of the major factors that influence the enrichment of ARGs in the soil. The results revealed the long-term risk of applying metal-enriched manure into agricultural soil, providing new parameters for the assessment of manure applications in agriculture.

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更新日期/Last Update: 2019-04-25