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[1]郝雯瑾,王悠,唐学玺.除菌处理对强壮前沟藻和青岛大扁藻生长的影响[J].应用与环境生物学报,2009,15(03):326-331.[doi:10.3724/SP.J.1145.2009.00326]
 HAO Wenjin,WANG You,TANG Xuexi.Effects of Axenation on Growth of Amphidinium carterae Hulburt and Platymonas helgolandica var. tsingtaoensis[J].Chinese Journal of Applied & Environmental Biology,2009,15(03):326-331.[doi:10.3724/SP.J.1145.2009.00326]
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除菌处理对强壮前沟藻和青岛大扁藻生长的影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
15卷
期数:
2009年03期
页码:
326-331
栏目:
研究论文
出版日期:
2009-05-15

文章信息/Info

Title:
Effects of Axenation on Growth of Amphidinium carterae Hulburt and Platymonas helgolandica var. tsingtaoensis
作者:
郝雯瑾王悠唐学玺
中国海洋大学海洋生命学院环境科学系 青岛 266003
Author(s):
HAO WenjinWANG YouTANG Xuexi
Ecology Laboratory, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, Shandong, China
关键词:
环境细菌抗生素除菌老化微藻除菌 强壮前沟藻青岛大扁藻
Keywords:
environmental bacterium antibiotic axenation aging nonaxenic algal Amphidinium carterae Hulburt Platymonas helgolandica var. tsingtaoensis
分类号:
Q949.208 : Q948.122.3
DOI:
10.3724/SP.J.1145.2009.00326
文献标志码:
A
摘要:
对微藻除菌以及未除菌与除菌后微藻生长特性的变化进行了研究. 采用抗生素(新霉素、链霉素和卡纳霉素)组合加入的方法,对微藻(强壮前沟藻和青岛大扁藻)进行了无菌化处理. 通过吖啶橙染色,镜检观察到与藻细胞共生的细菌经抗生素处理后数量明显减少,从而成功获得了2种无菌海洋微藻的纯藻种. 比较了抗生素除菌前后环境细菌对微藻生长的影响,结果表明,除菌后的强壮前沟藻与未除菌的相比,推迟2 d进入稳定期,处于稳定期的最大细胞密度比未除菌的提高了24%. 除菌后的强壮前沟藻和青岛大扁藻不易老化,可保持良好的悬浮状态. 图7 表1 参16
Abstract:
Abstract This paper presents the studies on microalgal axenation and the difference in growth between nonaxenic and axenic marine microalgae. Axenic cultures of two species of planktonic microalgae, Amphidinium carterae Hulburt and Platymonas helgolandica var. tsingtaoensis were obtained successfully through addition of antibiotics to microalgal culture medium, including ineomycin, streptomycin sulfate and Kanarese sulfate. Then, acridine orange staining and epifluorescence microscopy observation showed that symbiotic bacteria particles with algae cells were significantly reduced after antibiotic treatment. The results showed that for axenic A. carterae, the duration of exponential growth phase prolonged for two days, and the maximum population density increased by 24%, compared with nonaxenic ones. Both of axenic P. helgolandica and A. carterae could maintain suspension conditions, compared with nonaxenic ones, for which the phenomena of cell sinking were observed after 13 days of the culture. Fig 7, Tab 1, Ref 16

参考文献/References:

1 Mayali X, Doucette GJ. Microbial community interactions and population dynamics of an algicidal bacterium active against Karenia brevis (Dinophyceae). Harmful Algae, 2002, 1 (3): 277~293
2 Cottrell MT, Suttle CA. Production of axenic cultures of Micromonas pusilla (Prasinophyceae) using antibiotics. Phycology, 1993, 29 (3): 385~387
3 Spencer CP. On the uses of antibiotics for isolating bacteria-free cultures of marine phytoplankton organisms. Mar Biol Assoc UK, 1952, 31 (1): 97~106
4 Zobell CE, Long JH. Studies on the isolation of bacteria-free culture of marine phytoplankton. Mar Res, 1938, 1: 328~333
5 McCraeken IR. Purifying algal cultures: A review of chemical methods. Phycology, 1989, 27: 656~658
6 Lin W (林伟), Chen D (陈玛), Liu XY (刘秀云). Marine microalgal axenation and comparison of growth cheracteristics between natural and axenic marine microalgae. Oceanol ET Limnol Sin (海洋与湖沼) , 2000, 31 (6): 647~652
7 Droop MR. A procedure for routine purification of algal cultures with antibiotics. Eur J Phycol, 1967, 3 (2): 295~297
8 Martinez GV, Rodriguez MH. Strategy to obtain axenic cultures from field-collected samples of the cyanobacterium Phormidium animalis. Microbiol Methods, 2004, 57 (1): 115~121
9 Divan CL, Schnoes HK. Production of axenic Gonyaulax cultures by treatment with antibiotics. Appl Environ Microbiol, 1982, 44 (1): 250~254
10 Gallacher S, Smith EA. Bacteria and paralytic shellfish toxins. Protist, 1999, 150 (3): 245~255
11 Lu YH, Chai TJ, Hwang DF. Isolation of bacteria from dinoflagellate Alexandrium minutum and their effects on algae toxicity. Nat Toxins, 2000, 9 (4): 409~417
12 Droop MR, Elson KG. Are pelagic diatoms free from bacteria? Nature, 1966, 211: 1096~1097
13 CHU SP. Note on the technique of making bacteria-free culture of marine diatoms. Mar Biol AssocUK, 1946, 19: 755~759
14 Zobell CE, Long JH. Studies on the isolation of bacteria-free culture of marine phytoplankton. Mar Res, 1938, 1: 328~333
15 Maki T, Imai I. Relationships between intracellular bacteria and the bivalve killer dinoflagellate Heterocapsa circularisquama (Dinophyceae). Fish Sci, 2001, 67 (5): 794~803
16 Sakami T, Nakahara H, Chinain M. Effects of epiphytic bacteria on the growth of the toxic dinoflagellate Gambierdiscus toxicus (Dinophyceae). Exp Mar Biol & Ecol, 1999, 233 (2): 231~246

备注/Memo

备注/Memo:
*国家自然科学基金(No. 30270258和山东省自然科学基金(No. 2007ZRB01903)资助项目 Supported by the National Natural Science Foundation of China (No. 30270258), and the Natural Science Foundation of Shandong, China (No. 2007ZRB01903)
**通讯作者 Corresponding author (E-mail: tangxx@ouc.edu.cn)
更新日期/Last Update: 2009-07-03