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[1]王海东,刘韫滔,林伯坤,等.汕头海域几丁质酶产生菌的筛选及其几丁质酶编码基因研究[J].应用与环境生物学报,2009,15(04):511-514.[doi:10.3724/SP.J.1145.2009.00511]
 WANG Haidong,LIU Yuntao,LIN Bokun,et al.Isolation of Chitinolytic Bacteria from the Shantou Bay, China and Study on Their Chitinase Coding Genes[J].Chinese Journal of Applied & Environmental Biology,2009,15(04):511-514.[doi:10.3724/SP.J.1145.2009.00511]
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汕头海域几丁质酶产生菌的筛选及其几丁质酶编码基因研究()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
15卷
期数:
2009年04期
页码:
511-514
栏目:
研究论文
出版日期:
2009-08-25

文章信息/Info

Title:
Isolation of Chitinolytic Bacteria from the Shantou Bay, China and Study on Their Chitinase Coding Genes
作者:
王海东刘韫滔林伯坤宋小慧伦镜盛胡忠
王海东,刘韫滔,林伯坤,宋小慧,伦镜盛,胡忠
Author(s):
WANG HaidongLIU YuntaoLIN BokunSONG XiaohuiLun JingshengHU Zhong
1Department of Biology, Shantou University, Shantou 515063, Guangdong, China
2Xuzhou Bioengineering Higher Vocational School, Xuzhou 221006, Jiangsu, China
关键词:
汕头海域表层沉积物几丁质酶产生菌几丁质酶编码基因
Keywords:
Shantou Baysurface sedimentchitinolytic bacteriumchitinase coding gene
分类号:
Q936+Q78
DOI:
10.3724/SP.J.1145.2009.00511
文献标志码:
A
摘要:
在汕头海域表层沉积物中分离得到69株高产几丁质酶的菌株,对其中6株形态特征差异比较明显、几丁质酶活力比较高的菌株SWCH-1、SWCH-2、SWCH-3、SWCH-5、SWCH-6和SWCH-9进行了16S rDNA序列鉴定,发现它们分别归属于5个属,即短芽孢杆菌属(Brevibacillus)、类芽孢杆菌属(Paenibacillus)、芽孢杆菌属(Bacillus)、气单胞菌属(Aeromonas)和寡养单胞菌属(Stenotrophomonas). 采用兼并引物对6株菌几丁质酶编码基因的催化保守区片段进行了PCR扩增和序列分析,发现菌株均含有18家族几丁质酶编码基因;但是其编码的蛋白序列与NCBI收录的蛋白序列存在着差异,其中菌株SWCH-1和SWCH-3的蛋白序列与数据库中序列的相似性比较低,分别为85%和81%,菌株含有比较新的几丁质酶编码基因,其全基因序列的克隆和酶蛋白的纯化分析尚在进一步研究中. 图6 参18
Abstract:
69 chitinolytic bacteria were isolated from the surface sediments of the Shantou Bay in Guangdong, China. According to their colony characteristics and chitinase activities, six strains (named SWCH-1, SWCH-2, SWCH-3, SWCH-5, SWCH-6 and SWCH-9) were chosen for further study. Based on their 16S rDNA sequences they belonged to five genera: Brevibacillus, Paenibacillus, Bacillus, Aeromonas and Stenotrophomonas. The catalytic conservative region of their chitinase genes were amplified by PCR. And the result from DNA sequencing revealed that all their coding protein sequences were related to the 18th chitinase family, but their protein sequences were different from the highest homologous NCBI records. Strains SWCH-1 and SWCH-3 had lower similarities, 85% and 81%, respectively. Strains SWCH-1 and SWCH-3 might contain new chitinase coding genes. Fig 6, Ref 18

参考文献/References:

1 Lan XQ, Zhang X, Hu JH, Shimosaka M. Cloning, expreession, and characterization of a chitinase from the chitinolytic bacterium Aeromonas hydrophila strain SUWA-9. Biosci Biotechnal Biochem, 2006, 70 (10): 2437~2442
2 Kuk JH, Jung WJ, Jo GH, Kim YC, Kim KY, Park RD. Production of N-acetyl-β-D-glucosamine from chitin by Aeromonas sp. GJ-18 crude enzyme. Appl Microbiol Biotechnol, 2005, 68: 384~389
3 Benecke U. Bacillus chitinovorus eien chitin zersetzenden spaltpilz. Bot Ztg, 1995, 63: 22
4 Kurits K. Chemistry and application of chitin and chitiosan. Degradation & Stability, 1998, 59: 117
5 Hao JG (郝建国), Li J (李晶), Huang X (黄萱), Xu ZQ (徐子勤), Jia JF (贾敬芬), Han DJ (韩德俊), Zhao YW (赵宇玮). Transferring rice chitinase gene into wheat via microprojectile bombardment. Chin J Appl Environ Biol (应用与环境生物学报), 2004, 10 (4): 421~424
6 Suzuki K, Sugawara N, Suzuki M, Uchiyama T, Katouno F, Nikaidou N, Watanabe T. Chitinases A, B and C1 of Serratia marcescens 2170 produced by recombinant E. coli: Enzymatic properties and synergist on chitin degradation. Biosci Biotechnol Biochem, 2002, 66: 1075~1083
7 Huang XH (黄小红), Chen QX (陈清西), Wang J (王君), Sha L (沙莉), Huang ZP (黄志鹏), Guan X (关雄). Isolation, purification and properties of the chitinase from Bacillus thuingiensis. Chin J Appl Environ Biol (应用与环境生物学报), 2004, 10 (6): 771~773
8 Watanabe T, Suzuki K, Oyanagi W. Gene cloning of chitinase A1 from Bacillus circulans WL-12 revealed its evolutionary relationship to Serratia chitinase and to the type III homology units of bronectin. J Biol Chem, 1990, 65: 15659~15665
9 Lian MZ, Lin S, Zeng RY. Chitinase gene diversity at a deep sea station of the east Pacific nodule province. Extremophiles, 2007, 11: 463~467
10 Hitoshi S, Shizu F, Naoko Y, Norioki K, Atsuyoshi N, Einosuke M, Kazumi H, Kohei O, Aiba S. Production of N-acetyl-D-glucosamine from α-chitin by crude enzymes from Aeromonas hydrophila H-2330. Carbohyd Res, 2002, 337: 761~763
11 Kang SC, Park S, Lee DG. Purification and characterization of a novel chitinase from the entomopathogenic fungus Metarhizium anisopliae. J Invertebrate Pathol, 1999, 73: 276~281
12 Joseph S, David W. Molecular Cloning: A Labroatory Manual. 3rd ed. New York, USA: Cold Spring Harbor Laboratory Press, 2001
13 Hobel CFV, Marteinsson VT, Hreggvidsson GO, Kristjansson JK. Investigation of the microbial ecology of intertidal hot springs by using diversity analysis of 16S rRNA and chitinase genes. Appl Environ Microbiol, 2005, 71 (5): 2771~2776
14 Debaditya B, Anand N, Rajinder KG. Bacterial Chitinases: Properties and potential. Crit Rev Biotechnol, 2007, 27 (1): 21~28
15 Li DC. Review of fungal chitinases. Mycopathologia, 2006, 161: 345~360
16 Neetu D, Rupinder T, Gurinder SH. Biotechnological aspects of chitinolytic enzymes: A review. Appl Microbiol Biotechnol, 2006, 71: 773~782
17 Felse PA, Panda T. Regulation and cloning of microbial chitinase genes. Appl Microbiol Biotechnol, 1999, 51: 141~151
18 Cottrell MT, Moore TA, Kirchman DL. Chitinases from uncultured marine microorganisms. Appl Environl Microbiol, 1999, 65: 2553~2557

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备注/Memo

备注/Memo:
广东省科技计划项目(No. 2006B20501008)资助
更新日期/Last Update: 2009-08-26